MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
173 430.2782 429.2709
174 430.3036 429.2963
175 430.3046 429.2973
178 432.2579 431.2506
179 437.1978 436.1905
180 437.2155 436.2082
181 443.2483 442.2410
182 443.2489 442.2416
183 443.2492 442.2419
184 444.2941 443.2868
185 444.2943 443.2870
186 444.3190 443.3117
187 446.2737 445.2664
188 446.2740 445.2667
189 446.2988 445.2915
190 450.2472 449.2399
191 450.2724 449.2651
192 451.2676 450.2603
193 451.2679 450.2606
195 466.2427 465.2354
196 467.7737 466.7664
198 472.3268 471.3195
204 474.2933 473.2860
206 476.2114 475.2041
207 480.7645 479.7572
208 481.7763 480.7690
209 484.3284 483.3211
210 484.3287 483.3214
211 484.3288 483.3215
216 493.2787 492.2714
223 506.3101 505.3028
224 508.2890 507.2817
225 510.2376 509.2303
230 516.3135 515.3062
233 522.7798 521.7725
234 524.2370 523.2297
235 524.3098 523.3025
245 533.1208 532.1135
248 538.3157 537.3084
249 542.3613 541.3540
253 549.3015 548.2942
254 550.3007 549.2934
255 552.8260 551.8187
256 555.1038 554.0965
258 557.0791 556.0718
262 559.8188 558.8115
263 566.2949 565.2876
265 569.8051 568.7978
266 571.0677 570.0604
267 571.8585 570.8512
274 581.8284 580.8211
285 586.8321 585.8248
291 594.3337 593.3264
294 599.6761 598.6688
295 600.0101 599.0028
311 304.1623 606.3100
319 614.8373 613.8300
324 618.3572 617.3499
334 312.1857 622.3568
335 626.3162 625.3089
336 626.4210 625.4137
355 637.3109 636.3036
374 651.3011 650.2938
429 682.4675 681.4602
453 692.3840 691.3767
465 349.7017 697.3888
494 357.2140 712.4134
516 723.3608 722.3535
517 723.3613 722.3540
523 364.2176 726.4206
545 734.7263 733.7190
550 368.7280 735.4414
554 370.7033 739.3920
571 750.0923 749.0850
574 752.3960 751.3887
575 752.9005 751.8932
604 384.7067 767.3988
606 385.1935 768.3724
620 388.2328 774.4510
660 395.7095 789.4044
673 798.3824 797.3751
695 807.9429 806.9356
696 808.1221 807.1148
706 408.2176 814.4206
716 822.4064 821.3991
720 412.2444 822.4742
725 413.7441 825.4736
739 415.6942 829.3738
748 833.9116 832.9043
749 834.4506 833.4433
751 418.2336 834.4526
753 837.1236 836.1163
784 849.4680 848.4607
802 427.2022 852.3898
805 427.7416 853.4686
810 855.0719 854.0646
843 872.4432 871.4359
844 872.4576 871.4503
849 437.7466 873.4786
853 875.4658 874.4585
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