MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
70 359.2411 358.2338
71 359.2411 358.2338
72 359.2412 358.2339
73 359.2419 358.2346
74 359.2660 358.2587
75 360.2254 359.2181
76 361.1984 360.1911
77 361.2206 360.2133
78 365.2159 364.2086
79 367.2330 366.2257
80 367.2330 366.2257
81 367.2339 366.2266
82 367.2339 366.2266
83 369.2493 368.2420
84 369.2524 368.2451
85 371.2414 370.2341
86 371.2538 370.2465
87 371.3183 370.3110
88 371.3192 370.3119
89 371.3192 370.3119
90 371.3196 370.3123
91 371.3201 370.3128
92 371.3202 370.3129
93 371.3202 370.3129
94 373.2445 372.2372
95 373.2565 372.2492
96 373.2824 372.2751
97 374.2289 373.2216
98 374.2293 373.2220
99 374.2403 373.2330
100 375.2249 374.2176
101 375.2374 374.2301
102 376.2634 375.2561
103 376.2637 375.2564
104 385.2219 384.2146
105 385.7488 384.7415
106 386.2405 385.2332
107 387.2723 386.2650
108 387.2728 386.2655
109 388.2565 387.2492
110 389.2394 388.2321
111 389.2404 388.2331
112 389.2521 388.2448
113 393.2481 392.2408
114 393.2885 392.2812
115 393.3011 392.2938
116 393.3016 392.2943
117 395.2635 394.2562
118 398.2460 397.2387
119 398.2464 397.2391
120 399.2336 398.2263
121 399.2367 398.2294
122 401.2875 400.2802
123 401.2879 400.2806
124 401.2890 400.2817
125 401.7271 400.7198
126 402.2467 401.2394
127 402.2473 401.2400
129 403.2313 402.2240
130 405.7279 404.7206
131 407.2259 406.2186
132 407.2259 406.2186
133 407.2264 406.2191
134 407.2270 406.2197
135 407.2274 406.2201
136 407.2276 406.2203
137 407.2276 406.2203
138 407.2286 406.2213
139 409.2413 408.2340
140 409.2417 408.2344
141 409.2429 408.2356
142 409.2432 408.2359
143 409.2435 408.2362
144 409.2437 408.2364
145 409.2444 408.2371
146 410.2460 409.2387
147 413.2740 412.2667
148 413.7427 412.7354
149 415.2151 414.2078
150 416.2629 415.2556
151 416.2632 415.2559
152 416.2877 415.2804
153 416.2882 415.2809
154 416.2885 415.2812
155 416.7701 415.7628
157 417.2471 416.2398
159 418.2668 417.2595
160 421.2575 420.2502
161 423.2214 422.2141
162 423.2939 422.2866
163 423.7290 422.7217
164 425.2370 424.2297
165 425.2375 424.2302
166 425.2377 424.2304
167 425.2381 424.2308
168 425.2386 424.2313
169 425.2388 424.2315
170 427.2551 426.2478
171 428.2878 427.2805
172 429.2697 428.2624
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