MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3008 948.0045 1893.9944 1893.9982 -1.99 1 0 4 +1Score > 19 indicates identity HSVPTIQEIVNLQMLR + Deamidated (NQ); Oxidation (M)
3105 657.6888 1970.0446 1970.0394 2.63 1 0 3.8 +1Score > 19 indicates identity MSPSILVAQNLSKVVPSAE + Deamidated (NQ)
2900 609.6708 1825.9906 1825.9785 6.62 1 0 3.2 +1Score > 18 indicates identity INIRNLLQLNQGSVIE + 3 Deamidated (NQ)
4085 898.4499 2692.3279 2692.3313 -1.26 1 0 2.9 +1Score > 17 indicates identity NTQAMADDLFAMIGSGKLAVDIQQR
1274 354.8584 1061.5534 1061.5400 12.6 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology		 NRCAVVTSR
2099 489.2620 1464.7642 1464.7911 -18.4 1 0 4.4 +1Score > 19 indicates identity YGRMLAFAQALPK
3446 717.3662 2149.0768 2149.0473 13.7 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 NTQSGNPLPIVALTAHAMANE + Deamidated (NQ)
428 341.6986 681.3826 681.3810 2.49 0 0 2.2 +1Score > 16 indicates identity LQPAPR + Deamidated (NQ)
1126 990.5161 989.5088 989.5142 -5.39 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 LQSTIGSQR + Deamidated (NQ)
2878 907.4584 1812.9022 1812.9118 -5.29 1 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 FAALTNIRDPGQAQGPR + 2 Deamidated (NQ)
4405 1034.2118 3099.6136 3099.6227 -2.95 1 0 1.9 +1Score > 16 indicates identity ELGINTSINLVNSTVDTTPPFLNSLTIPK + 2 Deamidated (NQ)
1064 319.8391 956.4955 956.4927 2.92 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 AQLSNPGLR + 2 Deamidated (NQ)
1175 337.8669 1010.5789 1010.5873 -8.29 0 0 3.2 +1Score > 18 indicates identity AGQAIGKPLR + Deamidated (NQ)
2369 397.4733 1585.8641 1585.8749 -6.82 0 0 3.8 +1Score > 18 indicates identity TLAPDIILLDISMR + Oxidation (M)
671 399.2114 796.4082 796.4119 -4.62 1 0 2.8 +1Score > 17 indicates identity KDFPYK
875 887.4658 886.4585 886.4508 8.67 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 QGELVDAR
2021 474.6049 1420.7929 1420.7926 0.22 0 0 3.2 +1Score > 18 indicates identity IIHIDIDPASISK
3214 1017.0227 2032.0308 2032.0286 1.12 1 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 IETQVMSLTGLALGQLDLE + 2 Deamidated (NQ)
3998 862.4536 2584.3390 2584.3070 12.4 1 0 3.6 +1Score > 18 indicates identity TPFMVGGRLTIADLSLAGYVFMPE
2478 545.6359 1633.8859 1633.9093 -14.3 1 0 3 +1Score > 17 indicates identity DAALLLIGFWRGFR
2298 519.2970 1554.8692 1554.8842 -9.64 1 0 3 +1Score > 17 indicates identity LSGNSLRAGQVLALR + Deamidated (NQ)
2727 581.6653 1741.9741 1741.9760 -1.13 0 0 1.7 +1Score > 15 indicates identity LQVIAAVVIGGTSLMGGR + Deamidated (NQ)
240 530.3010 529.2937 529.2972 -6.62 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 ALGNR
2289 517.2710 1548.7912 1548.7970 -3.77 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 LSATAPGMAFGNGVIK + Oxidation (M)
2731 581.9999 1742.9779 1743.0045 -15.3 0 0 1.3 +1Score > 14 indicates identity TLLVLGMFFSYLLVK
2795 887.4505 1772.8864 1772.9057 -10.9 0 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 TQNTVVNFPIIADADR
2315 782.3931 1562.7716 1562.7801 -5.42 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 QGGRSGTGGFLQQLR + 2 Deamidated (NQ)
1700 627.3293 1252.6440 1252.6346 7.52 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 AHGPSLCATALR
3372 706.3605 2116.0597 2116.0840 -11.5 1 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology		 NAARSVDILLADYLPGWLE + Deamidated (NQ)
2617 565.2928 1692.8566 1692.8656 -5.32 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 TASTPHTTRPEPSRR
2915 460.7475 1838.9609 1838.9924 -17.1 0 0 4.6 +1Score > 19 indicates identity MSSINPVVVLPQALQAR + Deamidated (NQ); Oxidation (M)
1 302.1775 301.1702
2 302.2085 301.2012
3 303.1783 302.1710
4 303.1784 302.1711
5 303.1786 302.1713
6 303.2032 302.1959
7 304.1622 303.1549
8 304.1639 303.1566
9 304.8354 303.8281
10 305.0107 304.0034
11 309.2066 308.1993
12 310.1826 309.1753
13 313.2216 312.2143
14 315.6853 314.6780
15 317.1940 316.1867
16 317.2189 316.2116
17 319.1622 318.1549
18 319.2273 318.2200
19 321.2427 320.2354
20 322.1882 321.1809
21 323.2052 322.1979
22 325.2219 324.2146
23 331.2097 330.2024
24 331.2348 330.2275
25 332.8546 331.8473
26 333.1891 332.1818
27 333.2064 332.1991
28 333.2064 332.1991
29 333.2066 332.1993
30 333.2069 332.1996
31 333.2070 332.1997
32 333.2076 332.2003
33 333.2140 332.2067
34 335.2219 334.2146
35 335.2219 334.2146
36 335.2226 334.2153
37 335.2229 334.2156
38 335.2232 334.2159
39 335.2232 334.2159
40 335.2422 334.2349
41 337.2381 336.2308
42 337.2587 336.2514
43 340.6946 339.6873
44 345.2248 344.2175
45 345.2250 344.2177
46 345.2253 344.2180
47 345.2256 344.2183
48 345.2257 344.2184
49 349.2224 348.2151
50 349.2228 348.2155
51 351.2167 350.2094
52 351.2173 350.2100
53 351.2177 350.2104
54 351.2177 350.2104
55 351.2183 350.2110
56 351.2185 350.2112
57 351.2382 350.2309
58 351.2383 350.2310
59 351.2390 350.2317
60 351.7116 350.7043
61 353.2539 352.2466
62 353.2547 352.2474
63 353.6988 352.6915
64 356.2733 355.2660
65 357.2505 356.2432
66 358.2458 357.2385
67 359.2042 358.1969
68 359.2410 358.2337
69 359.2410 358.2337
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