| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 1004.0823 | 2006.1500 | 2006.1102 | 19.9 | 1 | 1 | 0.73 | 1Score > 13 indicates identity |
LVLPGVRFAVDAYVNFAR | |
| 607.3030 | 1212.5914 | 1212.5921 | -0.53 | 0 | 1 | 0.99 | 1Score > 21 indicates identityScore > 14 indicates homology |
LGLNLHMDGAR + Deamidated (NQ); Oxidation (M) | |
| 692.8197 | 1383.6248 | 1383.6050 | 14.3 | 1 | 1 | 3.2 | 1Score > 19 indicates identity |
DALVMACTLFED | |
| 556.6461 | 1666.9165 | 1666.9301 | -8.15 | 1 | 1 | 2.2 | 1Score > 17 indicates identity |
QQMLAIARALLNGPR + Oxidation (M) | |
| 703.8947 | 1405.7748 | 1405.7565 | 13.1 | 1 | 1 | 1.6 | 1Score > 16 indicates identity |
LEPALHASVAAKAE | |
| 601.3376 | 1200.6606 | 1200.6503 | 8.64 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
ELTAPFAVPTR | |
| 626.0083 | 1875.0031 | 1875.0254 | -11.9 | 1 | 1 | 2.8 | 1Score > 18 indicates identity |
LNLASAFGWQLKTLGQK + Deamidated (NQ) | |
| 880.4494 | 1758.8842 | 1758.9013 | -9.67 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 14 indicates homology |
QNLAPSQARLPAGIGHE + Deamidated (NQ) | |
| 898.4551 | 2692.3435 | 2692.3313 | 4.54 | 1 | 1 | 2.5 | 1Score > 18 indicates identity |
NTQAMADDLFAMIGSGKLAVDIQQR | |
| 638.9891 | 1913.9455 | 1913.9451 | 0.18 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
QLCAAGAQVLAVSRHMGK + 2 Deamidated (NQ); Oxidation (M) | |
| 467.7550 | 933.4954 | 933.4920 | 3.74 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 14 indicates homology |
VLPASEYR | |
| 772.4090 | 1542.8034 | 1542.8055 | -1.35 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 14 indicates homology |
QWGWLSINDIRR | |
| 721.7141 | 2162.1205 | 2162.1041 | 7.56 | 0 | 1 | 3.8 | 1Score > 19 indicates identity |
GPNTVSYGANALMAVVNILTR + 2 Deamidated (NQ) | |
| 550.9413 | 1649.8021 | 1649.7818 | 12.3 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 14 indicates homology |
INSLGAQTAINSQAMK + 4 Deamidated (NQ) | |
| 715.3983 | 2143.1731 | 2143.1572 | 7.41 | 1 | 1 | 1.6 | 1Score > 16 indicates identity |
GLMFVHNTLVGLNLRSSIR + Deamidated (NQ); Oxidation (M) | |
| 687.3584 | 2059.0534 | 2059.0415 | 5.79 | 1 | 1 | 0.82 | 1Score > 20 indicates identityScore > 13 indicates homology |
LEGVGGLKPGYYYYQPVR + Deamidated (NQ) | |
| 538.3165 | 1074.6184 | 1074.6219 | -3.23 | 1 | 1 | 1.6 | 1Score > 16 indicates identity |
MKALIVAASR + Oxidation (M) | |
| 923.4431 | 1844.8716 | 1844.8363 | 19.2 | 1 | 1 | 3.9 | 1Score > 19 indicates identity |
QGDPQHKGPNPCVAPIE + 2 Deamidated (NQ) | |
| 922.4886 | 1842.9626 | 1842.9734 | -5.83 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LGAMQVQRQADALSALR + Oxidation (M) | |
| 870.4727 | 1738.9308 | 1738.9287 | 1.22 | 1 | 1 | 3 | 1Score > 18 indicates identity |
LQRMGLPPLSQGALIE + Deamidated (NQ); Oxidation (M) | |
| 703.0196 | 2106.0370 | 2106.0381 | -0.56 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LQAQYLANLGFQPTGAQQR + 3 Deamidated (NQ) | |
| 635.8314 | 1269.6482 | 1269.6564 | -6.46 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
AGNIQSVNPAGLK + 2 Deamidated (NQ) | |
| 873.0016 | 1743.9886 | 1743.9632 | 14.6 | 1 | 1 | 0.89 | 1Score > 13 indicates identity |
QATAGKILFNGQDIIR | |
| 957.9722 | 3827.8597 | 3827.7896 | 18.3 | 0 | 1 | 1.3 | 1Score > 15 indicates identity |
AWDANGQLWFNPFNTDGFLADQILVNWQYKPR + 4 Deamidated (NQ) | |
| 323.8388 | 968.4946 | 968.4927 | 1.94 | 1 | 1 | 3.7 | 1Score > 19 indicates identity |
ELGHLSDAK | |
| 532.9813 | 1595.9221 | 1595.8995 | 14.2 | 1 | 1 | 1.3 | 1Score > 15 indicates identity |
LAQQNGKVVAALNAAK + Deamidated (NQ) | |
| 700.4064 | 1398.7982 | 1398.7871 | 7.98 | 1 | 1 | 2.5 | 1Score > 17 indicates identity |
ILGIGEQTLIWR + Deamidated (NQ) | |
| 515.6038 | 1543.7896 | 1543.8028 | -8.58 | 0 | 1 | 0.98 | 1Score > 21 indicates identityScore > 13 indicates homology |
LSPQTIAGACGLSLR + Deamidated (NQ) | |
| 920.4438 | 1838.8730 | 1838.8720 | 0.56 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
SDDSFVIMARTDALAVE | |
| 525.2556 | 2096.9933 | 2096.9650 | 13.5 | 1 | 1 | 4.1 | 1Score > 19 indicates identity |
RLQFDLGQYAQGQQGQVR + 6 Deamidated (NQ) | |
| 1098.5725 | 2195.1304 | 2195.1369 | -2.92 | 1 | 1 | 4.1 | 1Score > 20 indicates identity |
GRTIGLVPQDPMVSLNPTQR + Deamidated (NQ); Oxidation (M) | |
| 892.7770 | 2675.3092 | 2675.2941 | 5.63 | 1 | 1 | 3.8 | 1Score > 19 indicates identity |
YNIIMETALDSPHFADFPPDAALK | |
| 395.7099 | 789.4052 | 789.4055 | -0.26 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
AGAMSIPK + Oxidation (M) | |
| 538.3185 | 1611.9337 | 1611.9057 | 17.4 | 1 | 1 | 0.86 | 1Score > 13 indicates identity |
GALRQAVTLGLGSDVR | |
| 474.3055 | 473.2982 | 473.2962 | 4.37 | 1 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
AGKAK | |
| 488.2632 | 487.2559 | 487.2642 | -17.0 | 0 | 1 | 1 | 1Score > 24 indicates identityScore > 13 indicates homology |
AVGLE | |
| 874.4342 | 2620.2808 | 2620.2414 | 15.0 | 0 | 1 | 3.6 | 1Score > 19 indicates identity |
MHGLGNDFMVLDLVSQHAHIQPK + 2 Deamidated (NQ); 2 Oxidation (M) | |
| 356.2360 | 710.4574 | 710.4551 | 3.27 | 1 | 1 | 1.1 | 1Score > 14 indicates identity |
RLPGLR | |
| 439.8832 | 1316.6278 | 1316.6534 | -19.4 | 0 | 1 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
MQPATTLLVPTE + Deamidated (NQ); Oxidation (M) | |
| 687.8742 | 1373.7338 | 1373.7225 | 8.29 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
GVGLMGGIELVADK + Oxidation (M) | |
| 551.9561 | 1652.8465 | 1652.8747 | -17.1 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
HDVARIPLSYAQQR | |
| 853.7994 | 2558.3764 | 2558.4029 | -10.4 | 1 | 1 | 2.1 | 1Score > 16 indicates identity |
AQDPFLVLKGLSNCLLGVTSLALK + 2 Deamidated (NQ) | |
| 898.4457 | 2692.3153 | 2692.3313 | -5.94 | 1 | 1 | 2.8 | 1Score > 18 indicates identity |
NTQAMADDLFAMIGSGKLAVDIQQR | |
| 1030.5278 | 2059.0410 | 2059.0415 | -0.20 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LEGVGGLKPGYYYYQPVR + Deamidated (NQ) | |
| 1030.5325 | 2059.0504 | 2059.0415 | 4.37 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LEGVGGLKPGYYYYQPVR + Deamidated (NQ) | |
| 793.4221 | 1584.8296 | 1584.8359 | -3.95 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
DNIIVGDTTLTPGLR + Deamidated (NQ) | |
| 1134.0726 | 2266.1306 | 2266.0900 | 18.0 | 0 | 1 | 4.1 | 1Score > 19 indicates identity |
QVCAAPQQALGDVQLLDEPGR + 2 Deamidated (NQ) | |
| 566.2832 | 1130.5518 | 1130.5428 | 7.98 | 1 | 1 | 0.98 | 1Score > 22 indicates identityScore > 13 indicates homology |
DDAARTQAQR | |
| 452.2395 | 1353.6967 | 1353.6928 | 2.83 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
EDIAFLAPRPPE | |
| 755.3834 | 1508.7522 | 1508.7722 | -13.2 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
AVSIADLKTDNSFK + Deamidated (NQ) | |
| 966.8281 | 2897.4625 | 2897.4294 | 11.4 | 1 | 1 | 3.1 | 1Score > 18 indicates identity |
QVQPTLGLGAPTDQVFLLQESNRPGTE + 3 Deamidated (NQ) | |
| 570.2629 | 1138.5112 | 1138.5230 | -10.3 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
WLPSTCGYR | |
| 1134.0730 | 2266.1314 | 2266.0900 | 18.3 | 0 | 1 | 4.1 | 1Score > 19 indicates identity |
QVCAAPQQALGDVQLLDEPGR + 2 Deamidated (NQ) | |
| 1030.5303 | 2059.0460 | 2059.0415 | 2.23 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LEGVGGLKPGYYYYQPVR + Deamidated (NQ) | |
| 362.2429 | 722.4712 | 722.4803 | -12.5 | 1 | 1 | 0.87 | 1Score > 13 indicates identity |
KHLVVK | |
| 471.7310 | 1882.8949 | 1882.9095 | -7.74 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
MVDNLEPNVGTPSPIQR + Deamidated (NQ); Oxidation (M) | |
| 636.3368 | 1905.9886 | 1905.9805 | 4.26 | 1 | 1 | 3.4 | 1Score > 18 indicates identity |
LRDMGIEPFLIASCLR + Oxidation (M) | |
| 812.4413 | 2434.3021 | 2434.3334 | -12.9 | 0 | 1 | 2 | 1Score > 16 indicates identity |
TCALNIALLSVPAFVTVFWALK + Deamidated (NQ) | |
| 780.6532 | 3118.5837 | 3118.5216 | 19.9 | 0 | 1 | 2.5 | 1Score > 17 indicates identity |
VLGCPQVGLDDNFFALGGHSLMATQIVSR + Deamidated (NQ); Oxidation (M) | |
| 898.4351 | 2692.2835 | 2692.3313 | -17.7 | 1 | 1 | 3 | 1Score > 18 indicates identity |
NTQAMADDLFAMIGSGKLAVDIQQR | |
| 947.4995 | 1892.9844 | 1893.0029 | -9.77 | 1 | 1 | 3.8 | 1Score > 19 indicates identity |
NTTLARLLYANVITAME | |
| 624.1025 | 3115.4761 | 3115.4550 | 6.79 | 1 | 1 | 2.5 | 1Score > 17 indicates identity |
DQIVSFEQLVQQAYFAQVSLSSTGFYR + 5 Deamidated (NQ) | |
| 346.8469 | 1037.5189 | 1037.5294 | -10.2 | 0 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
GAAGFAAFAGAK | |
| 1134.0710 | 2266.1274 | 2266.0900 | 16.5 | 0 | 1 | 4.1 | 1Score > 19 indicates identity |
QVCAAPQQALGDVQLLDEPGR + 2 Deamidated (NQ) | |
| 314.8369 | 941.4889 | 941.4753 | 14.5 | 1 | 1 | 4 | 1Score > 19 indicates identity |
MRYISTR + Oxidation (M) | |
| 1017.0008 | 2031.9870 | 2031.9829 | 2.03 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
YYWYQLVGLKVINQDE + 2 Deamidated (NQ) | |
| 706.3635 | 2116.0687 | 2116.0656 | 1.43 | 0 | 1 | 0.99 | 1Score > 20 indicates identityScore > 13 indicates homology |
LMLQQMIDASQVNVNGVVR + 2 Deamidated (NQ) | |
| 746.4069 | 2236.1989 | 2236.1699 | 12.9 | 0 | 1 | 2.3 | 1Score > 17 indicates identity |
TDHALLLVNLGSPASTSVADVR + Deamidated (NQ) | |
| 898.4389 | 2692.2949 | 2692.3313 | -13.5 | 1 | 1 | 3 | 1Score > 18 indicates identity |
NTQAMADDLFAMIGSGKLAVDIQQR | |
| 1030.5413 | 2059.0680 | 2059.0415 | 12.9 | 1 | 1 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LEGVGGLKPGYYYYQPVR + Deamidated (NQ) | |
| 545.6313 | 1633.8721 | 1633.9039 | -19.5 | 0 | 1 | 3.2 | 1Score > 18 indicates identity |
LLAQNAALPPLQNLR + 3 Deamidated (NQ) | |
| 967.5350 | 1933.0554 | 1933.0244 | 16.1 | 1 | 1 | 2.5 | 1Score > 17 indicates identity |
LHTQPMDWLIKGQPIR + Deamidated (NQ) | |
| 696.7042 | 2087.0908 | 2087.0535 | 17.9 | 1 | 1 | 3.4 | 1Score > 18 indicates identity |
VGQNAKYDINILANGSPAIE + Deamidated (NQ) | |
| 940.4762 | 1878.9378 | 1878.9298 | 4.29 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
AGYDPNAAITLWNKMSK | |
| 544.7724 | 1087.5302 | 1087.5298 | 0.41 | 1 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
KDPFNPVNR + 2 Deamidated (NQ) | |
| 837.4710 | 1672.9274 | 1672.9083 | 11.4 | 1 | 0 | 1.7 | 1Score > 15 indicates identity |
FSLGMVPLSGLQPRR + Oxidation (M) | |
| 314.1957 | 626.3768 | 626.3864 | -15.2 | 0 | 0 | 2.5 | 1Score > 17 indicates identity |
VKPQR | |
| 423.7294 | 1690.8885 | 1690.8566 | 18.8 | 1 | 0 | 3.3 | 1Score > 18 indicates identity |
SDITEVAQTPLFWGK | |
| 852.1064 | 2553.2974 | 2553.3295 | -12.6 | 0 | 0 | 3.5 | 1Score > 18 indicates identity |
TPPIVQACLQSPAVTALLGVGTGMR + Deamidated (NQ); Oxidation (M) | |
| 898.4438 | 2692.3096 | 2692.3313 | -8.05 | 1 | 0 | 3 | 1Score > 18 indicates identity |
NTQAMADDLFAMIGSGKLAVDIQQR | |
| 455.5554 | 1363.6444 | 1363.6667 | -16.3 | 1 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
RQFGGGNGQMIAK + Deamidated (NQ) | |
| 1145.5787 | 3433.7143 | 3433.7202 | -1.74 | 1 | 0 | 2.3 | 1Score > 17 indicates identity |
MLRPPFRVLQMPRPAWSLYAYQLIEPDE + 2 Deamidated (NQ); Oxidation (M) | |
| 614.3244 | 613.3171 | 613.3071 | 16.3 | 0 | 0 | 2.2 | 1Score > 16 indicates identity |
AGLQPE | |
| 963.4724 | 1924.9302 | 1924.9564 | -13.6 | 0 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
QHKPVVMALNTQSSIAAE + 2 Deamidated (NQ) | |
| 546.8078 | 1091.6010 | 1091.5975 | 3.28 | 1 | 0 | 3.7 | 1Score > 19 indicates identity |
IQSILAEYR | |
| 468.9139 | 1403.7199 | 1403.7166 | 2.35 | 0 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
AMLMLQRPSWR + Oxidation (M) | |
| 488.7485 | 1950.9649 | 1950.9833 | -9.44 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LMHEQQVGSIVVVDPQR + Deamidated (NQ); Oxidation (M) | |
| 532.9438 | 1595.8096 | 1595.8267 | -10.7 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
SQLIDRAVDNLSHK + Deamidated (NQ) | |
| 408.2354 | 1221.6844 | 1221.6942 | -8.03 | 1 | 0 | 2.9 | 1Score > 18 indicates identity |
SRLLHGVANVR + Deamidated (NQ) | |
| 527.3021 | 1578.8845 | 1578.8590 | 16.1 | 1 | 0 | 2.1 | 1Score > 16 indicates identity |
QAVARLQGLGGGAPGAR + Deamidated (NQ) | |
| 545.9552 | 1634.8438 | 1634.8376 | 3.77 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
QGSGYQTRINQLLR + 2 Deamidated (NQ) | |
| 1083.5277 | 2165.0408 | 2165.0795 | -17.9 | 1 | 0 | 4.5 | 1Score > 19 indicates identity |
AFSQLQAPAQALMVRMVMR + 2 Deamidated (NQ); Oxidation (M) | |
| 920.4532 | 919.4459 | 919.4433 | 2.87 | 1 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
MNIEQLR + Deamidated (NQ); Oxidation (M) | |
| 481.2459 | 1440.7159 | 1440.7208 | -3.45 | 0 | 0 | 1 | 1Score > 21 indicates identityScore > 13 indicates homology |
SIAGNIQDIQGAPR + 2 Deamidated (NQ) | |
| 529.9625 | 1586.8657 | 1586.8450 | 13.0 | 1 | 0 | 3.5 | 1Score > 18 indicates identity |
QIMRVAGIDADLLR + Deamidated (NQ); Oxidation (M) | |
| 930.8062 | 2789.3968 | 2789.4494 | -18.9 | 1 | 0 | 3.5 | 1Score > 18 indicates identity |
LLPGRHPGMSSTQLQAALTTVGHTTSK + Deamidated (NQ) | |
| 1039.3535 | 6230.0773 | 6230.0459 | 5.04 | 1 | 0 | 0.93 | 1Score > 13 indicates identity |
FAVLLDDTSLEDGQGVAQQLLCTLDKPMFVDNQLINVTASVGLACAPLHGVDPASLMK + 3 Deamidated (NQ); 2 Oxidation (M) | |
| 643.3634 | 642.3561 | 642.3449 | 17.5 | 0 | 0 | 2.5 | 1Score > 17 indicates identity |
SLGNPR | |
| 400.7000 | 799.3854 | 799.3824 | 3.81 | 0 | 0 | 2.9 | 1Score > 17 indicates identity |
QQPAAQR + 2 Deamidated (NQ) | |
| 749.3975 | 1496.7804 | 1496.7947 | -9.52 | 1 | 0 | 1 | 1Score > 20 indicates identityScore > 13 indicates homology |
LRDVIPNTVQDAR + Deamidated (NQ) |
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