MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 341 |
314.6988 | 627.3830 | 627.3816 | 2.27 | 1 | 4 | 0.48 | 1Score > 14 indicates identity |
RISPR |
| 2187 |
501.9212 | 1502.7418 | 1502.7262 | 10.4 | 0 | 4 | 0.39 | 1Score > 21 indicates identity Score > 13 indicates homology |
SVQNMPGMPLWVK + Deamidated (NQ); Oxidation (M) |
| 2389 |
533.2811 | 1596.8215 | 1596.8219 | -0.30 | 1 | 4 | 0.53 | 1Score > 21 indicates identity Score > 14 indicates homology |
QALDDVQRQRPIR + 3 Deamidated (NQ) |
| 2618 |
424.2229 | 1692.8625 | 1692.8656 | -1.82 | 1 | 4 | 0.41 | 1Score > 21 indicates identity Score > 13 indicates homology |
TASTPHTTRPEPSRR |
| 3756 |
585.0383 | 2336.1241 | 2336.0923 | 13.6 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
IVRGATGMMQGAGNPSAAINMVR + 3 Deamidated (NQ); 2 Oxidation (M) |
| 3414 |
712.7114 | 2135.1124 | 2135.0971 | 7.16 | 1 | 4 | 1.4 | 1Score > 18 indicates identity |
ALNVTGNNIANVATTGFKSSR + Deamidated (NQ) |
| 2209 |
502.6058 | 1504.7956 | 1504.7807 | 9.91 | 1 | 4 | 0.83 | 1Score > 20 indicates identity Score > 16 indicates homology |
ILEMQTSAQLTLR + 2 Deamidated (NQ) |
| 814 |
429.2234 | 856.4322 | 856.4403 | -9.36 | 1 | 4 | 1.7 | 1Score > 19 indicates identity |
EAQGLPSR |
| 557 |
372.1884 | 742.3622 | 742.3610 | 1.73 | 0 | 4 | 1.3 | 1Score > 18 indicates identity |
GEPADVR |
| 2075 |
723.8687 | 1445.7228 | 1445.7402 | -12.0 | 1 | 4 | 0.88 | 1Score > 21 indicates identity Score > 16 indicates homology |
IVSFNLDKDPVAE |
| 1391 |
560.8049 | 1119.5952 | 1119.6036 | -7.49 | 1 | 4 | 0.76 | 1Score > 21 indicates identity Score > 16 indicates homology |
LNQGRFSGLK + Deamidated (NQ) |
| 3518 |
1096.5629 | 2191.1112 | 2191.0984 | 5.88 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
NVVVILMESFAGHFVGALGSE + Oxidation (M) |
| 3215 |
678.3541 | 2032.0405 | 2032.0034 | 18.2 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
GQITKAVADMALNLLDVDE + Deamidated (NQ); Oxidation (M) |
| 2639 |
568.6237 | 1702.8493 | 1702.8678 | -10.9 | 0 | 4 | 0.56 | 1Score > 21 indicates identity Score > 14 indicates homology |
LTAWEPQFIAATAQR + Deamidated (NQ) |
| 710 |
408.7083 | 815.4020 | 815.4137 | -14.3 | 0 | 4 | 0.85 | 1Score > 22 indicates identity Score > 16 indicates homology |
ILQNDGR + Deamidated (NQ) |
| 1297 |
358.5161 | 1072.5265 | 1072.5301 | -3.42 | 0 | 4 | 0.4 | 1Score > 22 indicates identity Score > 13 indicates homology |
LGAWASPQSR + Deamidated (NQ) |
| 2892 |
912.9805 | 1823.9464 | 1823.9417 | 2.58 | 1 | 4 | 1.8 | 1Score > 19 indicates identity |
LADEFYVTGAIINAATR |
| 3892 |
825.0925 | 2472.2557 | 2472.3006 | -18.2 | 1 | 4 | 1.5 | 1Score > 18 indicates identity |
MDSITQAVLGAALQGTVLGRIQGR + 2 Deamidated (NQ); Oxidation (M) |
| 1653 |
615.3650 | 1228.7154 | 1228.7040 | 9.28 | 1 | 4 | 0.82 | 1Score > 16 indicates identity |
KVHSHGGLPAVK |
| 795 |
426.7097 | 851.4048 | 851.4025 | 2.78 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
AADYGVEK |
| 2179 |
500.5881 | 1498.7425 | 1498.7160 | 17.7 | 1 | 4 | 0.4 | 1Score > 20 indicates identity Score > 13 indicates homology |
MLEITTMTSVWR + 2 Oxidation (M) |
| 128 |
403.2311 | 402.2238 | 402.2227 | 2.86 | 0 | 4 | 1 | 1Score > 31 indicates identity Score > 17 indicates homology |
AGAGK |
| 2351 |
790.4482 | 1578.8818 | 1578.8590 | 14.5 | 1 | 4 | 0.96 | 1Score > 17 indicates identity |
QAVARLQGLGGGAPGAR + Deamidated (NQ) |
| 1339 |
547.7845 | 1093.5544 | 1093.5590 | -4.17 | 0 | 4 | 0.5 | 1Score > 20 indicates identity Score > 14 indicates homology |
MNIPYSVVR + Oxidation (M) |
| 292 |
597.3410 | 596.3337 | 596.3282 | 9.26 | 0 | 4 | 0.99 | 1Score > 17 indicates identity |
EPPVR |
| 1466 |
383.8694 | 1148.5864 | 1148.5826 | 3.31 | 0 | 4 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
YGAIDLINDR |
| 1976 |
700.4069 | 1398.7992 | 1398.7831 | 11.6 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
LLLVNNGLTASQR + Deamidated (NQ) |
| 1413 |
564.8203 | 1127.6260 | 1127.6200 | 5.39 | 1 | 4 | 0.51 | 1Score > 20 indicates identity Score > 14 indicates homology |
IWLGRSPSGR |
| 1846 |
668.8315 | 1335.6484 | 1335.6419 | 4.90 | 0 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
YEPGVSVSGTGQR |
| 1250 |
351.5180 | 1051.5322 | 1051.5338 | -1.59 | 1 | 4 | 0.83 | 1Score > 21 indicates identity Score > 16 indicates homology |
EFGASGFIPK |
| 555 |
371.6966 | 741.3786 | 741.3882 | -12.8 | 0 | 4 | 1.8 | 1Score > 19 indicates identity |
QRPQGR + Deamidated (NQ) |
| 750 |
418.2263 | 834.4380 | 834.4236 | 17.4 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
VAVDEFR |
| 1349 |
366.8817 | 1097.6233 | 1097.6193 | 3.64 | 0 | 4 | 1.9 | 1Score > 19 indicates identity |
LNLQAGQINK |
| 4081 |
898.4437 | 2692.3093 | 2692.3313 | -8.16 | 1 | 4 | 1.3 | 1Score > 18 indicates identity |
NTQAMADDLFAMIGSGKLAVDIQQR |
| 2207 |
752.9026 | 1503.7906 | 1503.8032 | -8.35 | 1 | 4 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
SDSVSLIDVVTLKE |
| 3108 |
658.0025 | 1970.9857 | 1971.0214 | -18.1 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
ASVNFASTGYFQVKVTPR |
| 232 |
518.2979 | 517.2906 | 517.2860 | 8.95 | 1 | 4 | 0.79 | 1Score > 24 indicates identity Score > 15 indicates homology |
KQGGK + Deamidated (NQ) |
| 3375 |
706.3636 | 2116.0690 | 2116.0656 | 1.57 | 0 | 4 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
LMLQQMIDASQVNVNGVVR + 2 Deamidated (NQ) |
| 1739 |
426.5753 | 1276.7041 | 1276.6849 | 15.0 | 0 | 4 | 1.3 | 1Score > 17 indicates identity |
FLLMQLQDLR + Deamidated (NQ) |
| 2893 |
608.9906 | 1823.9500 | 1823.9417 | 4.51 | 1 | 4 | 2 | 1Score > 19 indicates identity |
LADEFYVTGAIINAATR |
| 819 |
429.7629 | 857.5112 | 857.5083 | 3.44 | 1 | 4 | 0.68 | 1Score > 20 indicates identity Score > 15 indicates homology |
GKVIGTQR |
| 1905 |
456.2770 | 1365.8092 | 1365.7980 | 8.20 | 0 | 4 | 0.43 | 1Score > 13 indicates identity |
QASLAPIVDLIAR |
| 177 |
432.2453 | 431.2380 | 431.2380 | 0.077 | 0 | 4 | 1 | 1Score > 22 indicates identity Score > 16 indicates homology |
LTAAG |
| 1924 |
686.8364 | 1371.6582 | 1371.6677 | -6.92 | 0 | 4 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
SVGGGDQARPCLR |
| 1992 |
703.8934 | 1405.7722 | 1405.7565 | 11.2 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
LEPALHASVAAKAE |
| 722 |
412.7543 | 823.4940 | 823.5028 | -10.6 | 0 | 4 | 0.43 | 1Score > 13 indicates identity |
RPSPVLR |
| 1115 |
982.4742 | 981.4669 | 981.4590 | 8.12 | 0 | 4 | 0.72 | 1Score > 20 indicates identity Score > 15 indicates homology |
IAFACGSQK + Deamidated (NQ) |
| 2027 |
474.9420 | 1421.8042 | 1421.7990 | 3.61 | 1 | 4 | 1 | 1Score > 16 indicates identity |
LDLPAPPSTRSLR |
| 3198 |
1012.5247 | 2023.0348 | 2023.0349 | -0.039 | 1 | 4 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
MIWGRFAAPIIYDADIR + Oxidation (M) |
| 2969 |
624.6476 | 1870.9210 | 1870.8908 | 16.1 | 1 | 4 | 0.53 | 1Score > 20 indicates identity Score > 13 indicates homology |
DAGQTADVGSIVQPNLER + 2 Deamidated (NQ) |
| 1248 |
349.8416 | 1046.5030 | 1046.5066 | -3.49 | 0 | 4 | 1 | 1Score > 21 indicates identity Score > 16 indicates homology |
MQAQPLSVR + 2 Deamidated (NQ); Oxidation (M) |
| 505 |
717.3850 | 716.3777 | 716.3705 | 10.1 | 0 | 4 | 1 | 1Score > 24 indicates identity Score > 16 indicates homology |
VPTDGTK |
| 3167 |
670.7043 | 2009.0911 | 2009.0728 | 9.10 | 0 | 4 | 1.1 | 1Score > 16 indicates identity |
VQVNQVPGGMISNLANQLK |
| 3488 |
724.7262 | 2171.1568 | 2171.1368 | 9.19 | 1 | 4 | 1.5 | 1Score > 18 indicates identity |
SSVGVDAQALIATQAPSMLRR + Deamidated (NQ) |
| 2347 |
526.3126 | 1575.9160 | 1575.8847 | 19.8 | 0 | 4 | 0.55 | 1Score > 13 indicates identity |
YPLVLVPGMLGFVR + Oxidation (M) |
| 3373 |
706.3613 | 2116.0621 | 2116.0656 | -1.69 | 0 | 4 | 0.49 | 1Score > 20 indicates identity Score > 13 indicates homology |
LMLQQMIDASQVNVNGVVR + 2 Deamidated (NQ) |
| 4429 |
1046.5573 | 3136.6501 | 3136.6127 | 11.9 | 1 | 4 | 1.2 | 1Score > 17 indicates identity |
AMNRYGLGLARPKPVNNQALIDAGYLYR + 3 Deamidated (NQ) |
| 1645 |
410.2277 | 1227.6613 | 1227.6533 | 6.50 | 1 | 3 | 0.48 | 1Score > 21 indicates identity Score > 13 indicates homology |
QGLEVMPVLNK + Deamidated (NQ) |
| 1119 |
328.5351 | 982.5835 | 982.5923 | -9.02 | 0 | 3 | 0.72 | 1Score > 15 indicates identity |
QAAGLLALAR |
| 3006 |
947.9982 | 1893.9818 | 1893.9982 | -8.64 | 1 | 3 | 2 | 1Score > 19 indicates identity |
HSVPTIQEIVNLQMLR + Deamidated (NQ); Oxidation (M) |
| 1619 |
304.6740 | 1214.6669 | 1214.6884 | -17.7 | 1 | 3 | 0.83 | 1Score > 21 indicates identity Score > 15 indicates homology |
NRAIFLTPAGR |
| 3687 |
767.0585 | 2298.1537 | 2298.1460 | 3.33 | 1 | 3 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
HIGALAMSEPNAGSDVVSMKLR + Oxidation (M) |
| 3272 |
515.7759 | 2059.0745 | 2059.0442 | 14.7 | 1 | 3 | 2.3 | 1Score > 20 indicates identity |
AIGILGTGMQAQMQLEQLR + 2 Deamidated (NQ) |
| 3576 |
1112.0238 | 2222.0330 | 2222.0280 | 2.27 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
NPYALWEVFNDPQTQVSGR + 2 Deamidated (NQ) |
| 464 |
348.7063 | 695.3980 | 695.3966 | 2.07 | 0 | 3 | 1 | 1Score > 16 indicates identity |
LDLHAK |
| 486 |
354.1785 | 706.3424 | 706.3432 | -1.07 | 0 | 3 | 0.64 | 1Score > 20 indicates identity Score > 14 indicates homology |
MIGTNR + Oxidation (M) |
| 2539 |
829.9375 | 1657.8604 | 1657.8828 | -13.5 | 1 | 3 | 0.65 | 1Score > 21 indicates identity Score > 14 indicates homology |
AIQWLIEQGVPFTR + Deamidated (NQ) |
| 3311 |
696.0250 | 2085.0532 | 2085.0942 | -19.7 | 1 | 3 | 0.57 | 1Score > 20 indicates identity Score > 13 indicates homology |
MSVIAGVFGALPPHRYSQR |
| 3416 |
1068.5659 | 2135.1172 | 2135.0971 | 9.45 | 1 | 3 | 1.9 | 1Score > 19 indicates identity |
ALNVTGNNIANVATTGFKSSR + Deamidated (NQ) |
| 1307 |
539.2655 | 1076.5164 | 1076.5358 | -18.0 | 0 | 3 | 0.88 | 1Score > 21 indicates identity Score > 15 indicates homology |
AQTMMPLIR + Deamidated (NQ); Oxidation (M) |
| 1898 |
683.8281 | 1365.6416 | 1365.6525 | -7.92 | 1 | 3 | 1 | 1Score > 21 indicates identity Score > 16 indicates homology |
ISGGGDKDSFIDR |
| 2594 |
839.4216 | 1676.8286 | 1676.8555 | -16.0 | 0 | 3 | 0.68 | 1Score > 21 indicates identity Score > 14 indicates homology |
LSAMLNRPLYDLDR + Deamidated (NQ) |
| 641 |
392.7331 | 783.4516 | 783.4603 | -11.0 | 1 | 3 | 1.2 | 1Score > 17 indicates identity |
RLSSPPK |
| 1138 |
332.8546 | 995.5420 | 995.5301 | 12.0 | 0 | 3 | 1.3 | 1Score > 17 indicates identity |
NWLHSALR |
| 1621 |
406.2027 | 1215.5863 | 1215.5666 | 16.2 | 1 | 3 | 1 | 1Score > 21 indicates identity Score > 16 indicates homology |
CKDNDVNPVR |
| 3555 |
736.7263 | 2207.1571 | 2207.1474 | 4.39 | 1 | 3 | 1.7 | 1Score > 18 indicates identity |
DLLGTLEVPADAYYGIQTLR |
| 1264 |
353.8618 | 1058.5636 | 1058.5720 | -7.94 | 1 | 3 | 1 | 1Score > 22 indicates identity Score > 16 indicates homology |
VNRAANATIK + 2 Deamidated (NQ) |
| 2814 |
596.6614 | 1786.9624 | 1786.9690 | -3.71 | 0 | 3 | 1.9 | 1Score > 19 indicates identity |
LGVGVHLGQTDGPLTPAR |
| 4665 |
722.7799 | 3608.8631 | 3608.8695 | -1.76 | 1 | 3 | 0.74 | 1Score > 14 indicates identity |
MRALLVAGSVLLISACSTLPDPDPNQAWIDLTR + Oxidation (M) |
| 1201 |
514.2891 | 1026.5636 | 1026.5611 | 2.53 | 0 | 3 | 2.1 | 1Score > 19 indicates identity |
IWAGGPSIAR |
| 1056 |
478.2596 | 954.5046 | 954.5022 | 2.55 | 1 | 3 | 2.1 | 1Score > 19 indicates identity |
VTESTPPPK |
| 2251 |
765.3801 | 1528.7456 | 1528.7508 | -3.35 | 0 | 3 | 0.91 | 1Score > 20 indicates identity Score > 15 indicates homology |
NLNDNIIINQLLE + 4 Deamidated (NQ) |
| 2036 |
712.8615 | 1423.7084 | 1423.6957 | 8.98 | 0 | 3 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
NNVQNVWVHGTR + Deamidated (NQ) |
| 2555 |
834.4700 | 1666.9254 | 1666.8963 | 17.5 | 0 | 3 | 1.2 | 1Score > 16 indicates identity |
ILNLLNIPQDMLPR + 2 Deamidated (NQ); Oxidation (M) |
| 2813 |
596.6558 | 1786.9456 | 1786.9498 | -2.39 | 1 | 3 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
QLALICRDLQLTVNK + 3 Deamidated (NQ) |
| 3639 |
1133.1184 | 2264.2222 | 2264.1947 | 12.2 | 1 | 3 | 1.2 | 1Score > 16 indicates identity |
MSLALVHSRAQVGVQAPAVSVE + Oxidation (M) |
| 3461 |
1078.0295 | 2154.0444 | 2154.0328 | 5.42 | 1 | 3 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
AENAATADVLVVSSAINPANPE + 2 Deamidated (NQ) |
| 3168 |
670.7072 | 2009.0998 | 2009.0728 | 13.4 | 0 | 3 | 0.97 | 1Score > 15 indicates identity |
VQVNQVPGGMISNLANQLK |
| 2504 |
412.2274 | 1644.8805 | 1644.8947 | -8.65 | 0 | 3 | 2.4 | 1Score > 19 indicates identity |
AAAVDHLAPVLNDLAR |
| 1829 |
441.9152 | 1322.7238 | 1322.7306 | -5.19 | 1 | 3 | 1.5 | 1Score > 17 indicates identity |
HGVLGLDSQLKR + Deamidated (NQ) |
| 3600 |
748.4085 | 2242.2037 | 2242.1780 | 11.5 | 1 | 3 | 1.4 | 1Score > 17 indicates identity |
GHLSPLDGLGPEAIGIPQLMAR + Deamidated (NQ) |
| 1599 |
402.2202 | 1203.6388 | 1203.6472 | -7.03 | 1 | 3 | 0.94 | 1Score > 20 indicates identity Score > 15 indicates homology |
QQAQFASLRR |
| 2613 |
564.6375 | 1690.8907 | 1690.8831 | 4.46 | 0 | 3 | 1.7 | 1Score > 18 indicates identity |
HGYNPLFLYGGVGLGK |
| 968 |
463.2259 | 924.4372 | 924.4375 | -0.26 | 0 | 3 | 0.68 | 1Score > 20 indicates identity Score > 14 indicates homology |
NIFMIDR + Deamidated (NQ); Oxidation (M) |
| 3180 |
671.3525 | 2011.0357 | 2011.0520 | -8.13 | 1 | 3 | 2.3 | 1Score > 19 indicates identity |
VIAAKMAADLAQVDPANAAR + Oxidation (M) |
| 1471 |
1151.5632 | 1150.5559 | 1150.5771 | -18.4 | 1 | 3 | 1 | 1Score > 21 indicates identity Score > 15 indicates homology |
GATQVFFPER |
| 3686 |
1150.0837 | 2298.1528 | 2298.1743 | -9.35 | 1 | 3 | 1 | 1Score > 20 indicates identity Score > 15 indicates homology |
VAGNIGIPGLYVTEDPGAVDAAAK + Deamidated (NQ) |
| 2281 |
515.9508 | 1544.8306 | 1544.8423 | -7.61 | 1 | 3 | 1 | 1Score > 20 indicates identity Score > 15 indicates homology |
AIVQGLSGFQGVGRR + Deamidated (NQ) |
| 1065 |
479.2552 | 956.4958 | 956.4927 | 3.30 | 1 | 3 | 0.63 | 1Score > 20 indicates identity Score > 13 indicates homology |
TINDPIER |
| 944 |
915.5262 | 914.5189 | 914.5185 | 0.45 | 0 | 3 | 1 | 1Score > 21 indicates identity Score > 15 indicates homology |
LVQLASQR + Deamidated (NQ) |
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