MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 2388 |
799.4169 | 1596.8192 | 1596.7930 | 16.5 | 1 | 7 | 0.25 | 1Score > 21 indicates identity Score > 13 indicates homology |
SLIAAELAQPHGSMR + Deamidated (NQ); Oxidation (M) |
| 2879 |
454.2386 | 1812.9253 | 1812.9404 | -8.31 | 0 | 7 | 0.24 | 1Score > 20 indicates identity Score > 13 indicates homology |
AVSANMALQVAPPTLSAR + Deamidated (NQ); Oxidation (M) |
| 1920 |
686.3441 | 1370.6736 | 1370.6466 | 19.7 | 1 | 7 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
VQLQDPAWQRE + 2 Deamidated (NQ) |
| 1170 |
505.2541 | 1008.4936 | 1008.4876 | 5.97 | 1 | 7 | 0.84 | 1Score > 21 indicates identity Score > 19 indicates homology |
TEDFIAGTR |
| 924 |
452.7679 | 903.5212 | 903.5178 | 3.83 | 0 | 7 | 0.54 | 1Score > 17 indicates identity |
LSAFPITR |
| 2178 |
750.3768 | 1498.7390 | 1498.7627 | -15.8 | 0 | 7 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
QVAGDVAGLVAVDQR + 2 Deamidated (NQ) |
| 2001 |
470.6084 | 1408.8034 | 1408.8191 | -11.1 | 1 | 7 | 0.52 | 1Score > 16 indicates identity |
LQHGTILGFPKAK |
| 1381 |
372.5349 | 1114.5829 | 1114.5982 | -13.8 | 1 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
LEQGTLGQIR + Deamidated (NQ) |
| 1014 |
471.7513 | 941.4880 | 941.4930 | -5.30 | 1 | 7 | 0.86 | 1Score > 19 indicates identity |
KVDAGPNGGK |
| 1319 |
362.1936 | 1083.5590 | 1083.5600 | -0.99 | 0 | 7 | 0.97 | 1Score > 19 indicates identity |
LFQATFSAAK + Deamidated (NQ) |
| 1982 |
701.8992 | 1401.7838 | 1401.7650 | 13.4 | 0 | 7 | 0.61 | 1Score > 17 indicates identity |
ALVNTVNTVGVMGK |
| 2481 |
545.9634 | 1634.8684 | 1634.8529 | 9.47 | 1 | 7 | 0.96 | 1Score > 19 indicates identity |
HHSKLFGLTIDPDR |
| 3505 |
545.7712 | 2179.0557 | 2179.0831 | -12.6 | 0 | 7 | 0.26 | 1Score > 20 indicates identity Score > 13 indicates homology |
GPNTVSYGANALMAVVNILTR + 3 Deamidated (NQ); Oxidation (M) |
| 1746 |
639.8353 | 1277.6560 | 1277.6537 | 1.84 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
LIDISDGAVTMK + Oxidation (M) |
| 1509 |
387.8897 | 1160.6473 | 1160.6302 | 14.7 | 1 | 7 | 0.84 | 1Score > 18 indicates identity |
LTSSPRFTPR |
| 1923 |
458.2264 | 1371.6574 | 1371.6639 | -4.73 | 1 | 7 | 0.31 | 1Score > 21 indicates identity Score > 14 indicates homology |
SRMMQPLLDHK + Deamidated (NQ); Oxidation (M) |
| 2274 |
771.9285 | 1541.8424 | 1541.8202 | 14.5 | 1 | 7 | 0.94 | 1Score > 19 indicates identity |
NALSVGHYLLEGLR + Deamidated (NQ) |
| 2675 |
860.4589 | 1718.9032 | 1718.8991 | 2.39 | 0 | 7 | 0.99 | 1Score > 20 indicates identity Score > 19 indicates homology |
QLRPQAWHVLILDE + 2 Deamidated (NQ) |
| 1859 |
672.8509 | 1343.6872 | 1343.6694 | 13.3 | 1 | 7 | 0.67 | 1Score > 21 indicates identity Score > 17 indicates homology |
QRWQQDLNTR |
| 602 |
384.2248 | 766.4350 | 766.4337 | 1.74 | 1 | 6 | 0.69 | 1Score > 17 indicates identity |
ALGIHEK |
| 861 |
440.7262 | 879.4378 | 879.4239 | 15.9 | 0 | 6 | 0.75 | 1Score > 21 indicates identity Score > 18 indicates homology |
WGYDALR |
| 241 |
530.3309 | 529.3236 | 529.3336 | -18.9 | 1 | 6 | 0.89 | 1Score > 27 indicates identity Score > 18 indicates homology |
LGGKR |
| 2687 |
863.9897 | 1725.9648 | 1725.9777 | -7.47 | 1 | 6 | 0.48 | 1Score > 16 indicates identity |
IALEPRLAIDDFVVR |
| 338 |
627.3843 | 626.3770 | 626.3864 | -14.9 | 1 | 6 | 0.64 | 1Score > 17 indicates identity |
KAGVPR |
| 2402 |
536.2887 | 1605.8443 | 1605.8726 | -17.6 | 1 | 6 | 0.98 | 1Score > 19 indicates identity |
AAQISLYLKLDAGSR + Deamidated (NQ) |
| 2583 |
836.9620 | 1671.9094 | 1671.9196 | -6.05 | 0 | 6 | 0.98 | 1Score > 19 indicates identity |
ALGVAFSTLIQAPDLR + Deamidated (NQ) |
| 1063 |
319.8390 | 956.4952 | 956.5080 | -13.4 | 0 | 6 | 0.31 | 1Score > 20 indicates identity Score > 14 indicates homology |
WLSPDALR |
| 2108 |
735.3729 | 1468.7312 | 1468.7020 | 19.9 | 1 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
GQMFLLTRYPDE |
| 2034 |
712.8318 | 1423.6490 | 1423.6653 | -11.4 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
VELAHAMADQLPE + Deamidated (NQ) |
| 2489 |
546.9947 | 1637.9623 | 1637.9577 | 2.80 | 0 | 6 | 0.23 | 1Score > 13 indicates identity |
INQLVVRPTLQTTR |
| 1819 |
660.3499 | 1318.6852 | 1318.6625 | 17.3 | 0 | 6 | 0.58 | 1Score > 21 indicates identity Score > 16 indicates homology |
KPVVLMTMGSQE |
| 713 |
409.2200 | 816.4254 | 816.4341 | -10.6 | 0 | 6 | 0.51 | 1Score > 23 indicates identity Score > 16 indicates homology |
QALLDTR + Deamidated (NQ) |
| 884 |
891.5003 | 890.4930 | 890.4862 | 7.71 | 0 | 6 | 1.1 | 1Score > 19 indicates identity |
GIFQDALK |
| 503 |
358.7013 | 715.3880 | 715.3864 | 2.26 | 1 | 6 | 0.83 | 1Score > 25 indicates identity Score > 18 indicates homology |
QLVEAR + Deamidated (NQ) |
| 2556 |
556.9429 | 1667.8069 | 1667.7825 | 14.6 | 0 | 6 | 0.28 | 1Score > 20 indicates identity Score > 13 indicates homology |
YVAAMGGADQVLTQAR + 2 Deamidated (NQ); Oxidation (M) |
| 290 |
593.3303 | 592.3230 | 592.3220 | 1.67 | 0 | 6 | 1.2 | 1Score > 20 indicates identity |
ALNFK + Deamidated (NQ) |
| 2038 |
712.8629 | 1423.7112 | 1423.6990 | 8.59 | 1 | 6 | 0.48 | 1Score > 20 indicates identity Score > 15 indicates homology |
CHSDPRNQLAVK |
| 1309 |
539.8002 | 1077.5858 | 1077.5818 | 3.73 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
QAKQFSQLK + Deamidated (NQ) |
| 1541 |
588.8251 | 1175.6356 | 1175.6186 | 14.5 | 1 | 6 | 1.2 | 1Score > 19 indicates identity |
AQLFQAQKLE + Deamidated (NQ) |
| 1966 |
695.8781 | 1389.7416 | 1389.7239 | 12.8 | 1 | 6 | 1.2 | 1Score > 19 indicates identity |
ITESILITDTGVE |
| 220 |
503.3190 | 502.3117 | 502.3115 | 0.46 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
SVVAK |
| 320 |
615.3490 | 614.3417 | 614.3500 | -13.5 | 1 | 6 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
SAGPRK |
| 1102 |
974.5215 | 973.5142 | 973.5080 | 6.41 | 0 | 6 | 1 | 1Score > 23 indicates identity Score > 18 indicates homology |
ISLQAQANK + 2 Deamidated (NQ) |
| 2109 |
490.6092 | 1468.8058 | 1468.8072 | -0.94 | 0 | 6 | 1.1 | 1Score > 19 indicates identity |
NTKPLKPTPMGLR + Deamidated (NQ); Oxidation (M) |
| 2304 |
520.2880 | 1557.8422 | 1557.8151 | 17.4 | 1 | 6 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
RNQIFALIGPNGAGK + 3 Deamidated (NQ) |
| 1120 |
492.3311 | 982.6476 | 982.6287 | 19.2 | 1 | 6 | 0.26 | 1Score > 13 indicates identity |
VVQGIARLK |
| 158 |
417.2496 | 416.2423 | 416.2383 | 9.64 | 0 | 6 | 0.9 | 1Score > 32 indicates identity Score > 18 indicates homology |
AAAGK |
| 1723 |
635.3870 | 1268.7594 | 1268.7452 | 11.2 | 1 | 6 | 0.26 | 1Score > 13 indicates identity |
LVLVTRGAVAAAE |
| 1860 |
448.9033 | 1343.6881 | 1343.7045 | -12.2 | 1 | 6 | 0.72 | 1Score > 21 indicates identity Score > 17 indicates homology |
TDLAQAELNLTR |
| 2307 |
520.9501 | 1559.8285 | 1559.8155 | 8.32 | 1 | 6 | 0.61 | 1Score > 20 indicates identity Score > 16 indicates homology |
LDALASETQTSGVIR |
| 1770 |
431.5468 | 1291.6186 | 1291.6264 | -6.09 | 1 | 6 | 1 | 1Score > 21 indicates identity Score > 18 indicates homology |
VVQAMNQMVEK + Oxidation (M) |
| 435 |
343.2101 | 684.4056 | 684.4170 | -16.6 | 0 | 6 | 0.38 | 1Score > 14 indicates identity |
IISQPK |
| 1576 |
596.7980 | 1191.5814 | 1191.5693 | 10.2 | 1 | 6 | 0.53 | 1Score > 22 indicates identity Score > 15 indicates homology |
TLQLSLMEQE + Deamidated (NQ) |
| 2812 |
894.4796 | 1786.9446 | 1786.9101 | 19.3 | 1 | 6 | 0.89 | 1Score > 20 indicates identity Score > 18 indicates homology |
EILANDINFGIGPAGTGK + Deamidated (NQ) |
| 1580 |
599.3258 | 1196.6370 | 1196.6401 | -2.53 | 0 | 6 | 0.59 | 1Score > 20 indicates identity Score > 16 indicates homology |
LASLLDSPEPR |
| 3443 |
1075.0177 | 2148.0208 | 2148.0521 | -14.6 | 1 | 6 | 1.4 | 1Score > 20 indicates identity |
DNGELIGMVQLFNIDDISR |
| 222 |
506.2662 | 505.2589 | 505.2496 | 18.4 | 0 | 6 | 0.88 | 1Score > 23 indicates identity Score > 18 indicates homology |
QGSSK |
| 3071 |
972.5194 | 1943.0242 | 1943.0186 | 2.90 | 0 | 6 | 1.4 | 1Score > 20 indicates identity |
VFNMNKPGALLNIVVGGAE + Deamidated (NQ) |
| 2233 |
759.3983 | 1516.7820 | 1516.7562 | 17.1 | 0 | 6 | 1 | 1Score > 21 indicates identity Score > 18 indicates homology |
FQLHLFAALAQQE + 2 Deamidated (NQ) |
| 3284 |
692.3541 | 2074.0405 | 2074.0430 | -1.20 | 0 | 6 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
LNLPTNSSISVTLSQDDLR + 2 Deamidated (NQ) |
| 2396 |
802.8887 | 1603.7628 | 1603.7664 | -2.25 | 1 | 6 | 0.49 | 1Score > 21 indicates identity Score > 15 indicates homology |
KGGSLQLCWQPTTE |
| 1765 |
645.3506 | 1288.6866 | 1288.6915 | -3.73 | 1 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
LLLALVQGFEGE + Deamidated (NQ) |
| 3247 |
1023.5359 | 2045.0572 | 2045.0793 | -10.8 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
GKLSVQFSIEPLGLSANQR + 2 Deamidated (NQ) |
| 2466 |
816.4126 | 1630.8106 | 1630.8216 | -6.69 | 1 | 5 | 0.92 | 1Score > 21 indicates identity Score > 18 indicates homology |
LPAFSDAYQRAVHR + Deamidated (NQ) |
| 931 |
454.7469 | 907.4792 | 907.4651 | 15.6 | 1 | 5 | 0.94 | 1Score > 20 indicates identity Score > 18 indicates homology |
QYIIENK + Deamidated (NQ) |
| 2007 |
471.2573 | 1410.7501 | 1410.7255 | 17.4 | 1 | 5 | 0.48 | 1Score > 21 indicates identity Score > 15 indicates homology |
QSAILEHPVFNR + Deamidated (NQ) |
| 398 |
335.7114 | 669.4082 | 669.4061 | 3.17 | 0 | 5 | 0.32 | 1Score > 13 indicates identity |
LVPDVK |
| 698 |
405.7279 | 809.4412 | 809.4508 | -11.8 | 1 | 5 | 0.99 | 1Score > 18 indicates identity |
HALKDAR |
| 2240 |
507.5850 | 1519.7332 | 1519.7453 | -7.97 | 0 | 5 | 0.56 | 1Score > 21 indicates identity Score > 15 indicates homology |
QMLANSGFLSRPAE |
| 2297 |
778.4402 | 1554.8658 | 1554.8365 | 18.9 | 1 | 5 | 1 | 1Score > 18 indicates identity |
NTPQALLDQLKASR + Deamidated (NQ) |
| 2350 |
790.4476 | 1578.8806 | 1578.8590 | 13.7 | 1 | 5 | 0.78 | 1Score > 17 indicates identity |
QAVARLQGLGGGAPGAR + Deamidated (NQ) |
| 1717 |
422.9207 | 1265.7403 | 1265.7166 | 18.7 | 1 | 5 | 0.53 | 1Score > 15 indicates identity |
KAPPPTGGMLAVK |
| 507 |
718.3814 | 717.3741 | 717.3843 | -14.2 | 1 | 5 | 1 | 1Score > 22 indicates identity Score > 18 indicates homology |
VEMAIR |
| 1513 |
388.2095 | 1161.6067 | 1161.5931 | 11.7 | 0 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
HFLFDISQR |
| 2327 |
523.9320 | 1568.7742 | 1568.7504 | 15.1 | 1 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
QTVSEHQLPLQMR + 3 Deamidated (NQ) |
| 2650 |
855.4508 | 1708.8870 | 1708.8672 | 11.6 | 1 | 5 | 0.82 | 1Score > 20 indicates identity Score > 17 indicates homology |
NAGQLYIPNVLSYEK + Deamidated (NQ) |
| 2106 |
735.3696 | 1468.7246 | 1468.7463 | -14.7 | 0 | 5 | 0.51 | 1Score > 21 indicates identity Score > 15 indicates homology |
QEPSVVPVSWWR |
| 3173 |
671.0174 | 2010.0304 | 2010.0357 | -2.63 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
MFAQLLLNAPLQAVHGDR + Deamidated (NQ); Oxidation (M) |
| 1448 |
380.5691 | 1138.6855 | 1138.6822 | 2.86 | 1 | 5 | 0.59 | 1Score > 15 indicates identity |
LEPLSGRLVR |
| 1341 |
547.7850 | 1093.5554 | 1093.5590 | -3.27 | 0 | 5 | 0.63 | 1Score > 21 indicates identity Score > 15 indicates homology |
DVLGYLCVR |
| 2859 |
904.4568 | 1806.8990 | 1806.9224 | -12.9 | 1 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
VQQVEHDISGQAQVLR + Deamidated (NQ) |
| 2943 |
619.6398 | 1855.8976 | 1855.8952 | 1.29 | 1 | 5 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
VNPDIQQTITFEHANK + 2 Deamidated (NQ) |
| 2422 |
806.9113 | 1611.8080 | 1611.8216 | -8.42 | 1 | 5 | 0.54 | 1Score > 21 indicates identity Score > 15 indicates homology |
SDDEPLLIDLNRGR |
| 715 |
411.2490 | 820.4834 | 820.4694 | 17.1 | 1 | 5 | 0.66 | 1Score > 16 indicates identity |
LKALFTE |
| 1512 |
581.8094 | 1161.6042 | 1161.5812 | 19.8 | 0 | 5 | 0.66 | 1Score > 21 indicates identity Score > 16 indicates homology |
STDAGQLMALR |
| 2113 |
736.8670 | 1471.7194 | 1471.7453 | -17.6 | 1 | 5 | 0.94 | 1Score > 21 indicates identity Score > 17 indicates homology |
TGVPLMIDPDRSR + Oxidation (M) |
| 1477 |
385.5653 | 1153.6741 | 1153.6931 | -16.5 | 1 | 5 | 0.85 | 1Score > 17 indicates identity |
RLLLAASASPR |
| 2444 |
808.4478 | 1614.8810 | 1614.8763 | 2.92 | 0 | 5 | 1.3 | 1Score > 18 indicates identity |
LVNVTMTDVQNLIR |
| 1842 |
444.5918 | 1330.7536 | 1330.7278 | 19.3 | 1 | 5 | 0.87 | 1Score > 17 indicates identity |
LMANVSRLLADK + Deamidated (NQ) |
| 3771 |
784.0865 | 2349.2377 | 2349.2587 | -8.94 | 1 | 5 | 0.92 | 1Score > 17 indicates identity |
QAAGLLALARDCGTLVVHTNIR + Deamidated (NQ) |
| 203 |
474.2843 | 473.2770 | 473.2710 | 12.7 | 1 | 5 | 1 | 1Score > 24 indicates identity Score > 17 indicates homology |
KGGGR |
| 2231 |
758.3654 | 1514.7162 | 1514.7365 | -13.4 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
IPAWQGGLSTAQQR + 3 Deamidated (NQ) |
| 1527 |
391.5401 | 1171.5985 | 1171.6085 | -8.52 | 0 | 5 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
QVNDAVTALIE |
| 1495 |
1158.5989 | 1157.5916 | 1157.5928 | -1.02 | 0 | 5 | 1 | 1Score > 22 indicates identity Score > 17 indicates homology |
VGSINDIAAIGE |
| 1472 |
384.8498 | 1151.5276 | 1151.5207 | 5.96 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 17 indicates homology |
QGNDIGTQYR + Deamidated (NQ) |
| 4427 |
784.9210 | 3135.6549 | 3135.6287 | 8.36 | 1 | 5 | 0.76 | 1Score > 16 indicates identity |
AMNRYGLGLARPKPVNNQALIDAGYLYR + 2 Deamidated (NQ) |
| 1490 |
578.8528 | 1155.6910 | 1155.6903 | 0.61 | 1 | 5 | 0.66 | 1Score > 15 indicates identity |
EILTPPIVFK |
| 964 |
922.5010 | 921.4937 | 921.4920 | 1.90 | 1 | 5 | 0.88 | 1Score > 20 indicates identity Score > 17 indicates homology |
NQFKGTVK + Deamidated (NQ) |
| 1031 |
316.1793 | 945.5161 | 945.5244 | -8.76 | 0 | 5 | 0.86 | 1Score > 21 indicates identity Score > 16 indicates homology |
AGSVVTVASR |
| 1812 |
439.8826 | 1316.6260 | 1316.6394 | -10.2 | 0 | 5 | 1 | 1Score > 21 indicates identity Score > 17 indicates homology |
IPGASNLVDMQR + Deamidated (NQ); Oxidation (M) |
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