MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4681 920.9961 3679.9553
4682 921.0053 3679.9921
4683 921.5012 3681.9757
4684 737.5754 3682.8406
4685 926.9877 3703.9217
4686 927.7234 3706.8645
4687 931.7571 3722.9993
4688 747.5949 3732.9381
4689 934.4782 3733.8837
4690 749.4083 3742.0051
4691 937.5129 3746.0225
4692 938.5241 3750.0673
4693 939.5184 3754.0445
4694 940.2590 3757.0069
4696 946.5005 3781.9729
4697 947.5122 3786.0197
4698 948.0090 3788.0069
4699 949.4962 3793.9557
4700 952.4672 3805.8397
4701 953.0334 3808.1045
4702 953.4409 3809.7345
4703 956.4944 3821.9485
4704 765.4037 3821.9821
4705 766.1923 3825.9251
4706 766.3793 3826.8601
4707 957.7599 3827.0105
4709 958.5220 3830.0589
4711 962.2434 3844.9445
4712 962.4973 3845.9601
4713 777.1979 3880.9531
4714 971.4965 3881.9569
4715 972.0013 3883.9761
4716 973.5016 3889.9773
4717 973.5054 3889.9925
4718 976.0057 3899.9937
4720 979.7126 3914.8213
4721 783.9734 3914.8306
4722 981.5264 3922.0765
4723 984.7545 3934.9889
4724 788.3998 3936.9626
4725 985.9659 3939.8345
4726 991.4544 3961.7885
4727 1000.9996 3999.9693
4728 801.8085 4004.0061
4729 1002.0098 4004.0101
4730 1002.0291 4004.0873
4731 801.8277 4004.1021
4732 1002.2548 4004.9901
4733 1002.2558 4004.9941
4734 1002.2755 4005.0729
4735 1002.2781 4005.0833
4736 1002.2798 4005.0901
4737 1002.2807 4005.0937
4738 1002.2811 4005.0953
4739 802.0269 4005.0981
4740 1002.2894 4005.1285
4741 1002.3018 4005.1781
4742 1002.5407 4006.1337
4743 1002.5420 4006.1389
4749 1005.7200 4018.8509
4750 804.7839 4018.8831
4751 1007.7537 4026.9857
4752 1008.0624 4028.2205
4753 1011.4960 4041.9549
4754 809.3992 4041.9596
4755 1015.5287 4058.0857
4756 1016.5142 4062.0277
4757 1016.5273 4062.0801
4758 1016.5289 4062.0865
4759 1016.5364 4062.1165
4760 1016.5371 4062.1193
4761 1016.5380 4062.1229
4762 813.4329 4062.1281
4763 1016.5407 4062.1337
4764 1016.7781 4063.0833
4765 1016.7819 4063.0985
4766 1016.7822 4063.0997
4767 814.5995 4067.9611
4768 1018.2463 4068.9561
4769 814.8018 4068.9726
4770 1018.2505 4068.9729
4771 1022.3127 4085.2217
4772 1022.3134 4085.2245
4773 820.2214 4096.0706
4775 1030.5336 4118.1053
4776 1030.5446 4118.1493
4777 1030.7826 4119.1013
4778 1030.7855 4119.1129
4779 826.2051 4125.9891
4780 1034.5546 4134.1893
4781 1035.5017 4137.9777
4782 1035.5125 4138.0209
4783 1037.0330 4144.1029
4784 831.4387 4152.1571
4785 831.6284 4153.1056
4786 693.1917 4153.1065
4787 831.6379 4153.1531
4788 1040.5413 4158.1361
4789 1044.0564 4172.1965
4790 1045.5676 4178.2413
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