MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4569 1112.5851 3334.7335
4570 1113.5947 3337.7623
4571 1114.6033 3340.7881
4572 836.2045 3340.7889
4573 1114.6106 3340.8100
4574 836.2150 3340.8309
4575 836.4161 3341.6353
4576 836.4262 3341.6757
4577 669.3598 3341.7626
4578 1114.9386 3341.7940
4579 836.4597 3341.8097
4580 836.6664 3342.6365
4581 838.2224 3348.8605
4582 1118.3098 3351.9076
4583 838.9875 3351.9209
4584 840.2131 3356.8233
4585 1120.9442 3359.8108
4586 1121.5745 3361.7017
4587 841.9534 3363.7845
4588 673.7695 3363.8111
4589 842.4598 3365.8101
4590 1124.5945 3370.7617
4592 844.4373 3373.7201
4593 676.7547 3378.7371
4596 1130.9240 3389.7502
4597 849.9243 3395.6681
4598 1133.2712 3396.7918
4599 681.1671 3400.7991
4601 1136.5251 3406.5535
4602 683.5933 3412.9301
4603 1142.9594 3425.8564
4604 1143.9191 3428.7355
4605 1143.9207 3428.7403
4606 858.4329 3429.7025
4607 686.9527 3429.7271
4608 858.4421 3429.7393
4609 1144.5967 3430.7683
4610 859.4302 3433.6917
4612 1148.2855 3441.8347
4613 1149.5753 3445.7041
4614 863.1840 3448.7069
4615 1157.5334 3469.5784
4617 694.9601 3469.7641
4618 868.4514 3469.7765
4619 1158.1885 3471.5437
4620 1158.5143 3472.5211
4621 869.4493 3473.7681
4622 1159.8760 3476.6062
4623 1163.2449 3486.7129
4624 872.7053 3486.7921
4625 872.7054 3486.7925
4626 872.7066 3486.7973
4627 872.7100 3486.8109
4628 872.9538 3487.7861
4629 872.9586 3487.8053
4630 874.7388 3494.9261
4631 875.1666 3496.6373
4632 1167.5624 3499.6654
4633 876.4572 3501.7997
4635 877.6769 3506.6785
4636 1171.6158 3511.8256
4637 878.9661 3511.8353
4638 706.1694 3525.8106
4639 882.4924 3525.9405
4640 882.4990 3525.9669
4641 883.9794 3531.8885
4642 1178.6362 3532.8868
4643 1178.6376 3532.8910
4644 1180.2622 3537.7648
4645 1181.6423 3541.9051
4646 886.9448 3543.7501
4647 887.7294 3546.8885
4648 1184.2372 3549.6898
4649 1185.6219 3553.8439
4650 711.9365 3554.6461
4651 889.9732 3555.8637
4652 890.4907 3557.9337
4653 891.4431 3561.7433
4654 713.9463 3564.6951
4656 892.4999 3565.9705
4657 893.4874 3569.9205
4658 714.9987 3569.9571
4659 715.1985 3570.9561
4660 715.2057 3570.9921
4661 894.0001 3571.9713
4662 894.4945 3573.9489
4663 894.4990 3573.9669
4664 894.7453 3574.9521
4668 603.3320 3613.9483
4669 906.4955 3621.9529
4670 906.7488 3622.9661
4671 726.1550 3625.7386
4672 908.4950 3629.9509
4673 909.4631 3633.8233
4674 911.7346 3642.9093
4675 913.2242 3648.8677
4676 914.0192 3652.0477
4678 917.4800 3665.8909
4679 919.0108 3672.0141
4680 919.4629 3673.8225
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