MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4450 792.4330 3165.7029
4451 1056.2417 3165.7033
4452 1057.9523 3170.8351
4453 1058.5356 3172.5850
4454 635.7050 3173.4886
4455 529.9229 3173.4937
4456 794.6568 3174.5981
4459 795.9317 3179.6977
4460 1061.5182 3181.5328
4461 796.3914 3181.5365
4462 1061.5212 3181.5418
4463 1061.5228 3181.5466
4466 1061.5267 3181.5583
4467 1061.5272 3181.5598
4468 1061.5337 3181.5793
4469 1061.5375 3181.5907
4471 796.4316 3181.6973
4472 1061.5747 3181.7023
4473 1061.8550 3182.5432
4474 796.8953 3183.5521
4475 1062.5675 3184.6807
4476 797.4196 3185.6493
4478 1063.8793 3188.6161
4479 798.4597 3189.8097
4480 1064.6134 3190.8184
4481 799.4258 3193.6741
4482 1066.2329 3195.6769
4483 800.1749 3196.6705
4484 800.4461 3197.7553
4485 1068.1614 3201.4624
4486 801.3737 3201.4657
4487 641.3580 3201.7536
4488 1068.5480 3202.6222
4489 641.5587 3202.7571
4490 1068.8577 3203.5513
4491 802.1664 3204.6365
4492 802.4200 3205.6509
4493 1070.2273 3207.6601
4494 1070.2281 3207.6625
4495 1070.5560 3208.6462
4496 1070.8676 3209.5810
4497 803.4055 3209.5929
4499 803.4112 3209.6157
4500 1070.8833 3209.6281
4501 1071.9098 3212.7076
4502 1071.9098 3212.7076
4503 804.4351 3213.7113
4504 1074.8865 3221.6377
4505 1075.1943 3222.5611
4506 1075.5283 3223.5631
4508 1077.5480 3229.6222
4509 1077.5481 3229.6225
4512 1078.8297 3233.4673
4513 1079.5284 3235.5634
4517 1079.8979 3236.6719
4518 810.4183 3237.6441
4519 810.4207 3237.6537
4520 1080.2260 3237.6562
4521 1080.5171 3238.5295
4522 1080.5227 3238.5463
4523 811.4058 3241.5941
4524 1082.5406 3244.6000
4526 812.4531 3245.7833
4527 1084.2646 3249.7720
4528 813.4586 3249.8053
4529 813.4595 3249.8089
4530 813.6555 3250.5929
4531 813.7029 3250.7825
4532 1085.2589 3252.7549
4533 652.5506 3257.7166
4534 815.6870 3258.7189
4535 1087.5525 3259.6357
4536 1088.2054 3261.5944
4537 816.4181 3261.6433
4538 1088.5361 3262.5865
4540 1089.6284 3265.8634
4541 1090.5031 3268.4875
4542 1092.5333 3274.5781
4543 819.6535 3274.5849
4544 820.4172 3277.6397
4545 1094.2112 3279.6118
4546 821.1533 3280.5841
4547 822.1640 3284.6269
4548 824.4512 3293.7757
4549 1099.7833 3296.3281
4550 1100.5691 3298.6855
4551 826.4068 3301.5981
4552 826.6583 3302.6041
4553 1103.2681 3306.7825
4554 827.7043 3306.7881
4555 662.3664 3306.7956
4556 827.7092 3306.8077
4558 827.9650 3307.8309
4560 662.7645 3308.7861
4561 1104.5682 3310.6828
4562 828.9130 3311.6229
4563 830.4222 3317.6597
4565 1107.2274 3318.6604
4567 1108.6008 3322.7806
4568 665.9485 3324.7061
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