MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4317 746.9120 2983.6189
4318 747.1385 2984.5249
4319 996.8359 2987.4859
4320 747.8795 2987.4889
4331 998.5012 2992.4818
4332 998.5020 2992.4842
4333 998.5021 2992.4845
4335 749.3744 2993.4685
4336 749.6389 2994.5265
4337 749.6394 2994.5285
4338 750.6486 2998.5653
4339 1001.4814 3001.4224
4340 1003.5107 3007.5103
4341 1003.5232 3007.5478
4343 603.0966 3010.4466
4344 1004.5253 3010.5541
4345 1005.5493 3013.6261
4348 1006.2422 3015.7048
4349 1006.5712 3016.6918
4350 755.1810 3016.6949
4351 756.0955 3020.3529
4352 756.3755 3021.4729
4353 1008.8694 3023.5864
4357 1010.5587 3028.6543
4358 1010.5606 3028.6600
4359 1011.1326 3030.3760
4360 760.1841 3036.7073
4361 1015.8708 3044.5906
4363 1017.5001 3049.4785
4364 1017.5013 3049.4821
4365 1017.5424 3049.6054
4366 1017.5645 3049.6717
4367 1018.5404 3052.5994
4368 1018.5445 3052.6117
4369 1019.5484 3055.6234
4370 764.9132 3055.6237
4371 765.6198 3058.4501
4372 765.6214 3058.4565
4373 766.1810 3060.6949
4374 1021.5234 3061.5484
4375 1021.5264 3061.5574
4376 1021.5286 3061.5640
4377 1022.2145 3063.6217
4378 1022.5389 3064.5949
4379 767.6538 3066.5861
4380 767.8548 3067.3901
4381 1023.5369 3067.5889
4383 1023.8484 3068.5234
4384 1023.9048 3068.6926
4385 1024.2352 3069.6838
4386 770.1865 3076.7169
4387 770.8819 3079.4985
4388 771.3931 3081.5433
4389 1028.5234 3082.5484
4390 1029.8997 3086.6773
4391 772.9274 3087.6805
4392 1030.8467 3089.5183
4393 1030.8973 3089.6701
4394 773.6691 3090.6473
4395 773.9198 3091.6501
4396 773.9240 3091.6669
4397 1031.8572 3092.5498
4403 1033.5355 3097.5847
4404 775.9105 3099.6129
4406 1034.2371 3099.6895
4407 1034.5061 3100.4965
4408 1035.8340 3104.4802
4409 1037.5658 3109.6756
4410 778.9194 3111.6485
4411 624.1018 3115.4726
4412 779.8755 3115.4729
4414 1040.2043 3117.5911
4416 1040.8894 3119.6464
4417 780.9203 3119.6521
4418 1041.2273 3120.6601
4419 1041.5011 3121.4815
4420 1041.5619 3121.6639
4421 781.6294 3122.4885
4422 1043.2520 3126.7342
4423 1044.2197 3129.6373
4424 784.3951 3133.5513
4425 784.3958 3133.5541
4426 1045.8585 3134.5537
4428 1046.2261 3135.6565
4431 1047.5620 3139.6642
4432 1050.5231 3148.5475
4434 788.1552 3148.5917
4435 788.1645 3148.6289
4436 1050.5544 3148.6414
4439 788.4027 3149.5817
4440 788.4030 3149.5829
4441 788.4115 3149.6169
4442 1051.5511 3151.6315
4443 789.9403 3155.7321
4444 790.4148 3157.6301
4445 1053.5541 3157.6405
4446 1055.5070 3163.4992
4447 1055.5117 3163.5133
4448 1055.5126 3163.5160
4449 1055.8452 3164.5138
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