MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4206 943.8594 2828.5564
4207 708.3718 2829.4581
4208 944.8084 2831.4034
4209 946.4799 2836.4179
4210 711.3430 2841.3429
4211 948.4973 2842.4701
4212 948.5457 2842.6153
4213 948.5554 2842.6444
4214 949.1475 2844.4207
4215 949.8118 2846.4136
4216 951.1245 2850.3517
4217 951.1396 2850.3970
4218 951.4508 2851.3306
4219 951.4517 2851.3333
4222 951.5549 2851.6429
4223 952.5309 2854.5709
4224 952.8783 2855.6131
4226 955.1301 2862.3685
4227 955.4619 2863.3639
4228 956.1459 2865.4159
4229 956.1570 2865.4492
4230 956.1602 2865.4588
4231 956.2175 2865.6307
4232 958.1790 2871.5152
4233 718.8876 2871.5213
4234 718.8877 2871.5217
4235 958.1912 2871.5518
4236 958.5125 2872.5157
4238 960.1414 2877.4024
4239 720.4006 2877.5733
4241 961.1293 2880.3661
4242 961.1296 2880.3670
4243 721.1530 2880.5829
4245 578.1177 2885.5521
4246 722.9183 2887.6441
4247 963.5564 2887.6474
4248 723.6340 2890.5069
4249 965.1566 2892.4480
4250 724.1195 2892.4489
4251 724.3743 2893.4681
4252 965.5219 2893.5439
4253 966.1478 2895.4216
4254 966.5192 2896.5358
4256 967.5081 2899.5025
4257 967.5226 2899.5460
4258 967.8411 2900.5015
4259 967.8511 2900.5315
4260 726.1550 2900.5909
4261 726.3878 2901.5221
4262 968.5178 2902.5316
4263 727.3638 2905.4261
4264 969.8212 2906.4418
4265 970.1446 2907.4120
4266 727.8623 2907.4201
4267 970.4768 2908.4086
4268 970.4866 2908.4380
4269 728.1322 2908.4997
4270 971.4388 2911.2946
4271 730.1245 2916.4689
4273 974.2103 2919.6091
4274 974.4760 2920.4062
4275 731.3937 2921.5457
4276 975.1920 2922.5542
4277 975.5264 2923.5574
4278 976.4991 2926.4755
4279 976.5253 2926.5541
4280 976.8395 2927.4967
4281 733.3945 2929.5489
4282 978.1954 2931.5644
4283 978.8267 2933.4583
4285 737.8721 2947.4593
4286 738.3998 2949.5701
4287 984.5250 2950.5532
4288 984.5313 2950.5721
4289 985.5256 2953.5550
4290 985.5287 2953.5643
4291 739.8844 2955.5085
4292 739.8844 2955.5085
4293 739.8861 2955.5153
4294 739.8882 2955.5237
4295 739.8898 2955.5301
4296 739.8910 2955.5349
4297 740.1359 2956.5145
4298 986.5338 2956.5796
4299 986.5339 2956.5799
4300 986.5356 2956.5850
4301 988.4846 2962.4320
4302 988.4858 2962.4356
4303 741.6169 2962.4385
4304 988.7838 2963.3296
4305 988.7858 2963.3356
4306 988.8177 2963.4313
4307 741.8708 2963.4541
4308 990.1912 2967.5518
4309 990.5190 2968.5352
4310 990.5203 2968.5391
4311 990.5367 2968.5883
4312 991.5163 2971.5271
4313 993.5211 2977.5415
4316 995.5450 2983.6132
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