MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4084 898.4495 2692.3267
4087 900.4796 2698.4170
4088 900.7863 2699.3371
4089 901.2006 2700.5800
4090 901.4577 2701.3513
4091 676.3540 2701.3869
4093 901.8009 2702.3809
4094 676.6338 2702.5061
4095 902.4703 2704.3891
4096 902.4786 2704.4140
4099 903.4787 2707.4143
4100 903.4816 2707.4230
4101 903.4880 2707.4422
4102 903.8071 2708.3995
4103 678.6013 2710.3761
4104 904.4681 2710.3825
4105 904.4753 2710.4041
4106 904.4767 2710.4083
4107 907.8215 2720.4427
4108 907.8226 2720.4460
4109 908.0752 2721.2038
4110 909.8254 2726.4544
4112 910.4972 2728.4698
4113 910.8527 2729.5363
4114 910.8561 2729.5465
4115 911.4884 2731.4434
4116 911.7847 2732.3323
4117 912.1090 2733.3052
4119 913.1347 2736.3823
4123 913.5065 2737.4977
4124 915.1472 2742.4198
4125 916.4874 2746.4404
4126 916.7764 2747.3074
4127 916.8456 2747.5150
4128 917.5217 2749.5433
4129 688.6349 2750.5105
4130 917.8458 2750.5156
4131 917.8470 2750.5192
4132 917.8476 2750.5210
4133 917.8496 2750.5270
4134 918.1844 2751.5314
4136 689.6165 2754.4369
4138 690.3711 2757.4553
4139 920.4669 2758.3789
4140 690.6038 2758.3861
4141 921.1531 2760.4375
4142 922.1303 2763.3691
4144 922.8364 2765.4874
4145 922.8428 2765.5066
4146 692.5922 2766.3397
4148 924.5293 2770.5661
4149 925.8365 2774.4877
4151 926.5052 2776.4938
4152 926.5079 2776.5019
4153 927.4618 2779.3636
4154 695.8588 2779.4061
4155 927.4777 2779.4113
4156 696.1108 2780.4141
4157 557.0917 2780.4221
4159 929.4454 2785.3144
4160 929.4958 2785.4656
4161 929.5063 2785.4971
4162 929.5139 2785.5199
4163 697.8696 2787.4493
4164 698.1138 2788.4261
4165 930.5074 2788.5004
4166 698.3536 2789.3853
4168 931.1328 2790.3766
4169 931.1963 2790.5671
4170 931.5164 2791.5274
4171 698.8976 2791.5613
4172 931.5334 2791.5784
4174 932.1823 2793.5251
4177 934.8411 2801.5015
4179 701.8619 2803.4185
4180 936.8177 2807.4313
4181 936.8448 2807.5126
4182 702.8892 2807.5277
4183 702.8893 2807.5281
4184 702.8907 2807.5337
4185 702.8921 2807.5393
4186 703.1362 2808.5157
4187 703.1440 2808.5469
4188 703.1441 2808.5473
4190 938.4724 2812.3954
4191 938.5087 2812.5043
4192 938.7889 2813.3449
4193 939.1795 2814.5167
4194 939.5167 2815.5283
4195 939.5197 2815.5373
4196 940.5166 2818.5280
4197 705.8461 2819.3553
4198 941.1249 2820.3529
4199 941.1345 2820.3817
4200 941.5058 2821.4956
4201 941.8481 2822.5225
4202 942.1215 2823.3427
4203 942.5109 2824.5109
4204 943.1499 2826.4279
4205 943.1615 2826.4627
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