MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3949 842.0650 2523.1732
3950 631.8021 2523.1793
3951 842.1372 2523.3898
3953 843.4239 2527.2499
3954 633.5572 2530.1997
3956 844.4273 2530.2601
3957 844.4316 2530.2730
3958 844.4357 2530.2853
3959 845.8249 2534.4529
3960 846.0925 2535.2557
3961 846.7883 2537.3431
3962 635.8196 2539.2493
3963 847.8133 2540.4181
3964 509.6464 2543.1956
3965 636.8065 2543.1969
3966 849.4347 2545.2823
3967 849.7955 2546.3647
3968 849.8264 2546.4574
3969 850.0673 2547.1801
3970 851.4537 2551.3393
3971 851.4719 2551.3939
3973 853.7509 2558.2309
3975 854.1160 2559.3262
3976 854.1220 2559.3442
3978 855.1285 2562.3637
3979 642.0771 2564.2793
3980 642.5952 2566.3517
3981 856.8091 2567.4055
3982 857.1452 2568.4138
3983 857.1472 2568.4198
3984 858.7125 2573.1157
3985 516.2684 2576.3056
3987 860.0729 2577.1969
3988 645.3342 2577.3077
3989 645.8103 2579.2121
3990 646.0555 2580.1929
3992 861.1161 2580.3265
3993 861.1428 2580.4066
3994 861.1487 2580.4243
3995 646.3382 2581.3237
3996 861.4746 2581.4020
3997 861.4868 2581.4386
3999 862.7432 2585.2078
4000 863.4351 2587.2835
4002 864.4803 2590.4191
4004 864.7387 2591.1943
4005 648.8074 2591.2005
4006 649.0909 2592.3345
4007 865.4575 2593.3507
4009 650.3464 2597.3565
4010 650.3482 2597.3637
4011 651.1164 2600.4365
4012 522.2744 2606.3356
4013 652.5994 2606.3685
4014 870.4606 2608.3600
4016 873.0847 2616.2323
4017 873.4059 2617.1959
4019 874.4375 2620.2907
4020 874.4800 2620.4182
4021 875.1435 2622.4087
4022 875.4751 2623.4035
4023 876.1531 2625.4375
4024 876.7433 2627.2081
4025 876.7444 2627.2114
4028 878.4479 2632.3219
4029 878.7794 2633.3164
4030 659.8138 2635.2261
4031 879.4162 2635.2268
4035 879.4461 2635.3165
4037 661.0824 2640.3005
4038 882.0942 2643.2608
4040 882.7784 2645.3134
4041 662.5942 2646.3477
4042 883.4759 2647.4059
4043 883.7465 2648.2177
4044 663.0620 2648.2189
4045 530.6513 2648.2201
4046 883.8078 2648.4016
4048 884.4899 2650.4479
4049 884.5087 2650.5043
4050 885.1270 2652.3592
4052 885.4722 2653.3948
4054 887.4412 2659.3018
4055 887.4655 2659.3747
4057 889.7972 2666.3698
4058 890.1556 2667.4450
4059 890.8245 2669.4517
4061 669.3015 2673.1769
4062 669.3214 2673.2565
4064 893.1479 2676.4219
4066 894.4796 2680.4170
4067 895.4497 2683.3273
4069 895.4780 2683.4122
4070 895.7526 2684.2360
4071 672.0678 2684.2421
4072 672.0688 2684.2461
4073 672.5947 2686.3497
4074 897.1635 2688.4687
4076 673.3471 2689.3593
4077 898.4244 2692.2514
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