MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3810 795.4430 2383.3072
3811 795.7142 2384.1208
3812 795.7164 2384.1274
3813 796.0902 2385.2488
3816 797.0829 2388.2269
3817 1195.2095 2388.4044
3818 797.1431 2388.4075
3820 797.4335 2389.2787
3821 797.4462 2389.3168
3822 599.2942 2393.1477
3826 800.0855 2397.2347
3827 600.3173 2397.2401
3828 800.1084 2397.3034
3829 800.1176 2397.3310
3830 800.4542 2398.3408
3833 801.4227 2401.2463
3835 802.3912 2404.1518
3836 802.7262 2405.1568
3838 803.0695 2406.1867
3839 803.0901 2406.2485
3840 803.4016 2407.1830
3841 803.4534 2407.3384
3844 604.3298 2413.2901
3845 805.8047 2414.3923
3846 806.0811 2415.2215
3847 806.4099 2416.2079
3848 605.3099 2417.2105
3849 484.4502 2417.2146
3850 605.3123 2417.2201
3851 806.7766 2417.3080
3852 807.7282 2420.1628
3853 606.2992 2421.1677
3854 808.4507 2422.3303
3860 813.1025 2436.2857
3861 813.1035 2436.2887
3862 813.1057 2436.2953
3863 813.1059 2436.2959
3864 813.4411 2437.3015
3865 813.7870 2438.3392
3866 813.7922 2438.3548
3868 814.3982 2440.1728
3869 611.2939 2441.1465
3870 611.2946 2441.1493
3871 611.2957 2441.1537
3873 816.4405 2446.2997
3874 816.7527 2447.2363
3875 817.7676 2450.2810
3876 819.7766 2456.3080
3877 820.1124 2457.3154
3878 820.7579 2459.2519
3879 821.1111 2460.3115
3880 821.1187 2460.3343
3881 616.3392 2461.3277
3882 822.1078 2463.3016
3883 822.1162 2463.3268
3884 822.4446 2464.3120
3885 822.7783 2465.3131
3886 823.0596 2466.1570
3887 617.5836 2466.3053
3891 824.1021 2469.2845
3896 826.1038 2475.2896
3898 620.0807 2476.2937
3899 826.7862 2477.3368
3900 826.7876 2477.3410
3901 827.4213 2479.2421
3902 827.4241 2479.2505
3903 828.1082 2481.3028
3904 828.4147 2482.2223
3905 621.5631 2482.2233
3906 621.5652 2482.2317
3908 622.8303 2487.2921
3909 830.4622 2488.3648
3910 830.7617 2489.2633
3911 830.7974 2489.3704
3912 831.4421 2491.3045
3913 831.7576 2492.2510
3914 624.0878 2492.3221
3915 832.0884 2493.2434
3916 832.1175 2493.3307
3920 624.5889 2494.3265
3922 832.7456 2495.2150
3923 833.7295 2498.1667
3924 625.5508 2498.1741
3930 835.7703 2504.2891
3931 837.0901 2508.2485
3932 628.0793 2508.2881
3933 837.1098 2508.3076
3934 628.3214 2509.2565
3935 837.4507 2509.3303
3936 837.4579 2509.3519
3937 837.4635 2509.3687
3938 837.4727 2509.3963
3940 629.3148 2513.2301
3941 839.1366 2514.3880
3942 840.4327 2518.2763
3943 630.5869 2518.3185
3944 631.0957 2520.3537
3945 841.7839 2522.3299
3946 631.5906 2522.3333
3947 841.7866 2522.3380
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