MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3664 763.3728 2287.0966
3665 763.4335 2287.2787
3666 1145.0609 2288.1072
3667 763.7509 2288.2309
3672 574.3120 2293.2189
3675 765.4245 2293.2517
3676 765.4273 2293.2601
3677 765.4290 2293.2652
3678 765.4295 2293.2667
3679 766.0562 2295.1468
3681 1149.1410 2296.2674
3682 766.7622 2297.2648
3683 766.7634 2297.2684
3684 1149.6432 2297.2718
3685 766.7678 2297.2816
3688 767.7502 2300.2288
3689 767.7530 2300.2372
3696 768.3783 2302.1131
3702 1152.0718 2302.1290
3703 769.0328 2304.0766
3716 1154.0608 2306.1070
3717 769.7106 2306.1100
3718 769.7385 2306.1937
3719 770.0387 2307.0943
3720 1154.5923 2307.1700
3721 578.0319 2308.0985
3722 770.4502 2308.3288
3725 1155.6554 2309.2962
3726 771.0570 2310.1492
3727 579.5421 2314.1393
3728 772.4170 2314.2292
3729 772.7207 2315.1403
3730 773.3970 2317.1692
3731 773.4510 2317.3312
3732 773.7590 2318.2552
3733 773.7590 2318.2552
3734 1160.5789 2319.1432
3735 774.0551 2319.1435
3736 774.0910 2319.2512
3737 774.1092 2319.3058
3738 774.3656 2320.0750
3740 774.7530 2321.2372
3741 774.7961 2321.3665
3742 1162.0956 2322.1766
3743 1162.5845 2323.1544
3744 775.4000 2323.1782
3746 776.4138 2326.2196
3747 776.7434 2327.2084
3748 777.4082 2329.2028
3749 777.4464 2329.3174
3750 777.6920 2330.0542
3751 779.0134 2334.0184
3752 779.4146 2335.2220
3753 584.8132 2335.2237
3755 779.7151 2336.1235
3758 1170.0989 2338.1832
3759 780.4036 2338.1890
3760 1170.5735 2339.1324
3761 780.7188 2339.1346
3764 781.1096 2340.3070
3766 1173.0261 2344.0376
3767 782.3548 2344.0426
3768 587.0193 2344.0481
3769 1173.6862 2345.3578
3770 782.7947 2345.3623
3772 784.4603 2350.3591
3773 785.7710 2354.2912
3774 786.4385 2356.2937
3775 786.7576 2357.2510
3776 590.3205 2357.2529
3777 787.4174 2359.2304
3778 591.0112 2360.0157
3779 787.7532 2360.2378
3781 788.1175 2361.3307
3782 788.7114 2363.1124
3783 1182.5638 2363.1130
3784 1182.5642 2363.1138
3785 788.7140 2363.1202
3786 1182.5797 2363.1448
3787 788.7228 2363.1466
3788 788.7257 2363.1553
3790 790.7623 2369.2651
3792 475.4733 2372.3301
3793 594.0901 2372.3313
3794 1190.6383 2379.2620
3795 595.8229 2379.2625
3796 794.0950 2379.2632
3797 1190.6396 2379.2646
3798 794.0955 2379.2647
3799 794.0958 2379.2656
3800 794.0969 2379.2689
3801 1190.6418 2379.2690
3802 794.0970 2379.2692
3803 794.0984 2379.2734
3804 794.0986 2379.2740
3805 794.1032 2379.2878
3806 794.1061 2379.2965
3807 794.1093 2379.3061
3808 794.7578 2381.2516
3809 795.3784 2383.1134
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