MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
922 452.2440 902.4734 902.4570 18.3 1 12 0.69 +1Score > 23 indicates identity
Score > 23 indicates homology		 QAAQRATR + 2 Deamidated (NQ)
3566 554.2687 2213.0457 2213.0786 -14.9 1 12 0.36 +1Score > 20 indicates identity LSPSGMPNPEVYLPSANGDLR
1008 470.7905 939.5664 939.5641 2.55 1 12 0.14 +1Score > 16 indicates identity DIPILIKE
634 390.2020 778.3894 778.3861 4.31 1 12 0.18 +1Score > 22 indicates identity
Score > 17 indicates homology		 GLAFNEK + Deamidated (NQ)
989 311.1790 930.5152 930.5246 -10.2 1 11 0.56 +1Score > 21 indicates identity SLAQLSRR + Deamidated (NQ)
1585 399.8909 1196.6509 1196.6401 9.04 1 11 0.33 +1Score > 19 indicates identity LLLNQQNPEK + Deamidated (NQ)
251 545.3220 544.3147 544.3220 -13.4 0 11 0.8 +1Score > 23 indicates identity ANLVK + Deamidated (NQ)
990 466.2658 930.5170 930.4995 18.9 1 11 0.56 +1Score > 21 indicates identity IASQRSNR
2406 804.8942 1607.7738 1607.7937 -12.4 1 11 0.21 +1Score > 21 indicates identity
Score > 17 indicates homology		 LNTLTMRDTSDAVR + Oxidation (M)
762 421.2674 840.5202 840.5181 2.54 0 11 0.19 +1Score > 17 indicates identity QALLLQR
470 351.2038 700.3930 700.3868 8.97 1 11 0.61 +1Score > 23 indicates identity
Score > 22 indicates homology		 VANLER
197 471.2692 470.2619 470.2601 3.84 0 11 0.076 +1Score > 13 indicates identity AGAPR
1142 333.1645 996.4717 996.4777 -6.06 0 11 0.1 +1Score > 20 indicates identity
Score > 14 indicates homology		 WQAHALDR + Deamidated (NQ)
569 749.4335 748.4262 748.4232 4.08 0 11 0.37 +1Score > 19 indicates identity QVFSLR
314 307.6801 613.3456 613.3548 -14.8 0 11 0.24 +1Score > 17 indicates identity QPITR
1595 601.3384 1200.6622 1200.6462 13.3 0 11 0.4 +1Score > 20 indicates identity
Score > 19 indicates homology		 SQDTAALVAAVR
950 460.2498 918.4850 918.4705 15.8 1 11 0.57 +1Score > 21 indicates identity MNTQARAK
980 465.2664 928.5182 928.5342 -17.1 0 11 0.21 +1Score > 21 indicates identity
Score > 16 indicates homology		 QALDLITR
1671 413.2118 1236.6136 1236.6027 8.82 1 11 0.12 +1Score > 21 indicates identity
Score > 14 indicates homology		 QEGFDAVFVPK + Deamidated (NQ)
1891 682.3718 1362.7290 1362.7255 2.57 1 11 0.44 +1Score > 20 indicates identity QQKKPAPVDHSK + Deamidated (NQ)
1296 536.7878 1071.5610 1071.5560 4.71 1 11 0.71 +1Score > 22 indicates identity DINALEQIR + Deamidated (NQ)
1644 614.8379 1227.6612 1227.6571 3.36 1 11 0.61 +1Score > 21 indicates identity QVDLRQAQLR + 2 Deamidated (NQ)
231 517.3134 516.3061 516.3020 8.01 0 10 0.57 +1Score > 23 indicates identity
Score > 21 indicates homology		 AGLTR
271 579.3297 578.3224 578.3176 8.27 0 10 0.49 +1Score > 20 indicates identity FGSIR
936 456.7625 911.5104 911.5150 -4.99 1 10 0.37 +1Score > 18 indicates identity KLYMTLK + Oxidation (M)
973 464.2735 926.5324 926.5185 15.0 0 10 0.3 +1Score > 18 indicates identity QVLSPLNR + Deamidated (NQ)
804 854.4753 853.4680 853.4770 -10.5 0 10 0.21 +1Score > 16 indicates identity AQGLVNPR
2409 804.8983 1607.7820 1607.7937 -7.26 1 10 0.63 +1Score > 21 indicates identity LNTLTMRDTSDAVR + Oxidation (M)
1601 602.8370 1203.6594 1203.6387 17.3 1 10 0.41 +1Score > 19 indicates identity FLIELQAQLE + Deamidated (NQ)
2572 835.4965 1668.9784 1668.9848 -3.80 1 10 0.095 +1Score > 13 indicates identity LGGLNEVLIVLLMAAK + Oxidation (M)
328 311.6593 621.3040 621.2945 15.4 0 10 0.32 +1Score > 18 indicates identity WMTGK
536 366.2194 730.4242 730.4337 -13.0 0 10 0.14 +1Score > 21 indicates identity
Score > 14 indicates homology		 ALSATLR
3655 760.4016 2278.1830 2278.1858 -1.25 1 10 0.46 +1Score > 19 indicates identity HIEPFVFQTARNLALDHLR + 2 Deamidated (NQ)
982 465.2763 928.5380 928.5454 -7.90 1 10 0.41 +1Score > 21 indicates identity
Score > 19 indicates homology		 AQRLATLR + Deamidated (NQ)
1761 644.3527 1286.6908 1286.7081 -13.4 1 10 0.3 +1Score > 20 indicates identity
Score > 17 indicates homology		 QALSSLLENALK + Deamidated (NQ)
293 597.3418 596.3345 596.3282 10.6 0 10 0.27 +1Score > 17 indicates identity EPPVR
440 343.7139 685.4132 685.4122 1.46 0 10 0.5 +1Score > 22 indicates identity
Score > 19 indicates homology		 NAIAAVK
1399 374.5374 1120.5904 1120.5989 -7.60 1 10 0.26 +1Score > 20 indicates identity
Score > 17 indicates homology		 QDHQRVPLK + Deamidated (NQ)
3447 717.3854 2149.1344 2149.1275 3.19 0 10 0.4 +1Score > 18 indicates identity TSLAFLSLMLQDMQPSLVR
2615 846.9536 1691.8926 1691.8883 2.59 0 10 0.45 +1Score > 19 indicates identity IFGRPVSLVLADYNE
1424 566.2872 1130.5598 1130.5819 -19.5 0 10 0.47 +1Score > 22 indicates identity
Score > 19 indicates homology		 TPLQSAGQLSK + 2 Deamidated (NQ)
4594 845.9437 3379.7457 3379.7874 -12.3 0 9 0.17 +1Score > 14 indicates identity AYNLPSAPLATTLNQIASQAGIALAIDPALVSGR + 3 Deamidated (NQ)
2866 604.2859 1809.8359 1809.8493 -7.43 0 9 0.15 +1Score > 20 indicates identity
Score > 14 indicates homology		 QAQASGFIDGQSLSGSTR + Deamidated (NQ)
2654 571.3152 1710.9238 1710.9264 -1.55 0 9 0.56 +1Score > 19 indicates identity LLAGQQQDLAISVQAR + Deamidated (NQ)
3893 825.4123 2473.2151 2473.1761 15.7 1 9 0.49 +1Score > 19 indicates identity YSDNGTSVSSVNFPEVALPAHPGK + Deamidated (NQ)
3571 740.3826 2218.1260 2218.1463 -9.16 0 9 0.46 +1Score > 18 indicates identity FLPFFITQSLGAFNDNLFK
1320 542.7869 1083.5592 1083.5600 -0.73 0 9 0.56 +1Score > 19 indicates identity LFQATFSAAK + Deamidated (NQ)
1568 594.8212 1187.6278 1187.6285 -0.57 0 9 0.4 +1Score > 22 indicates identity
Score > 18 indicates homology		 VGDIITITLNE + Deamidated (NQ)
2087 730.4050 1458.7954 1458.7864 6.20 0 9 0.59 +1Score > 19 indicates identity TIMLQNIAANITR + Deamidated (NQ)
218 501.3160 500.3087 500.3071 3.28 0 9 0.4 +1Score > 24 indicates identity
Score > 17 indicates homology		 VAGVR
2220 756.8702 1511.7258 1511.7077 12.0 1 9 0.75 +1Score > 20 indicates identity QAPDAGAQQDARQR + Deamidated (NQ)
215 489.2799 488.2726 488.2707 3.96 0 9 0.72 +1Score > 24 indicates identity
Score > 20 indicates homology		 SGGLR
1722 635.3802 1268.7458 1268.7452 0.49 1 9 0.22 +1Score > 15 indicates identity LVLVTRGAVAAAE
2031 712.3643 1422.7140 1422.7103 2.64 1 9 0.43 +1Score > 21 indicates identity
Score > 18 indicates homology		 QVGLGLEQGHDLR + 2 Deamidated (NQ)
1983 702.8665 1403.7184 1403.7045 9.96 0 9 0.83 +1Score > 21 indicates identity QVYSLALDNPQR + Deamidated (NQ)
827 430.7229 859.4312 859.4287 2.93 0 9 0.42 +1Score > 23 indicates identity
Score > 17 indicates homology		 IGGTVGDIE
1217 517.7491 1033.4836 1033.4723 11.0 1 9 0.58 +1Score > 21 indicates identity
Score > 19 indicates homology		 CNNANARSK
3145 666.3812 1996.1218 1996.0849 18.5 1 9 0.22 +1Score > 15 indicates identity LGLVGPRVAVNMAGGVIMIE + Deamidated (NQ)
1176 337.8706 1010.5900 1010.5985 -8.42 1 9 0.37 +1Score > 17 indicates identity QSIPAAIRR
2415 537.6244 1609.8514 1609.8498 0.98 0 9 0.6 +1Score > 19 indicates identity MSQVYSVAVVVGSLR + Oxidation (M)
2015 472.5766 1414.7080 1414.7052 1.94 1 9 0.31 +1Score > 21 indicates identity
Score > 16 indicates homology		 TLDTQTGQPVEAR
4068 895.4687 2683.3843 2683.3759 3.14 1 8 0.42 +1Score > 17 indicates identity FPAGYPVATVNEVIHDSGQPFAIVR
1329 363.2054 1086.5944 1086.5782 14.9 1 8 0.67 +1Score > 21 indicates identity
Score > 19 indicates homology		 TGTLTEGRPR
4244 962.7993 2885.3761 2885.4170 -14.2 1 8 0.57 +1Score > 18 indicates identity QMQAWKQHYQPFGPLSINLAGAQLR + 4 Deamidated (NQ)
3178 671.3513 2011.0321 2011.0197 6.17 0 8 0.7 +1Score > 19 indicates identity MFAQLLLNAPLQAVHGDR + 2 Deamidated (NQ); Oxidation (M)
745 417.2203 832.4260 832.4113 17.8 1 8 0.71 +1Score > 22 indicates identity
Score > 19 indicates homology		 KPEMQGK + Oxidation (M)
1884 453.5820 1357.7242 1357.6990 18.5 1 8 0.77 +1Score > 20 indicates identity
Score > 20 indicates homology		 NASQVELVWVGR + Deamidated (NQ)
1414 565.2885 1128.5624 1128.5411 18.9 1 8 0.95 +1Score > 21 indicates identity
Score > 21 indicates homology		 AENGGLINNVE
340 628.3691 627.3618 627.3592 4.22 0 8 0.45 +1Score > 17 indicates identity VTAQPI
3179 1006.5244 2011.0342 2011.0520 -8.84 1 8 0.71 +1Score > 19 indicates identity VIAAKMAADLAQVDPANAAR + Oxidation (M)
1109 488.7802 975.5458 975.5290 17.2 0 8 0.51 +1Score > 18 indicates identity LLGFGQWR
1172 337.5265 1009.5577 1009.5556 2.02 0 8 0.4 +1Score > 17 indicates identity ADLPPQTLR
547 368.2249 734.4352 734.4327 3.50 0 8 0.23 +1Score > 14 indicates identity LTFQVK
2653 856.4456 1710.8766 1710.8472 17.2 0 8 0.32 +1Score > 20 indicates identity
Score > 16 indicates homology		 VDALAASAGSVAVCGAHR
297 301.1692 600.3238 600.3344 -17.5 0 8 0.62 +1Score > 21 indicates identity
Score > 18 indicates homology		 AGQGLR
2493 547.2914 1638.8524 1638.8730 -12.6 0 8 0.27 +1Score > 20 indicates identity
Score > 15 indicates homology		 IPGTVWVVDQQQLR + Deamidated (NQ)
777 423.2414 844.4682 844.4766 -9.94 1 8 0.91 +1Score > 23 indicates identity
Score > 20 indicates homology		 ISRNVQK + Deamidated (NQ)
1450 380.8907 1139.6503 1139.6550 -4.15 0 8 0.54 +1Score > 18 indicates identity QVLISANGPIK + Deamidated (NQ)
495 357.2200 712.4254 712.4231 3.24 1 8 0.57 +1Score > 18 indicates identity ALQPKR + Deamidated (NQ)
911 450.2908 898.5670 898.5712 -4.62 1 8 0.33 +1Score > 15 indicates identity ALASRLLR
3133 664.3451 1990.0135 1990.0119 0.77 1 8 1 +1Score > 20 indicates identity
Score > 20 indicates homology		 YTVNAAGDPVATGRLITNR + 2 Deamidated (NQ)
626 389.1931 776.3716 776.3851 -17.3 0 8 0.58 +1Score > 21 indicates identity
Score > 18 indicates homology		 DLGGMLR + Oxidation (M)
3867 814.3860 2440.1362 2440.1097 10.8 1 8 0.62 +1Score > 18 indicates identity LSQMQTQISETNVILSMDNNR + 3 Deamidated (NQ); Oxidation (M)
2743 877.4625 1752.9104 1752.8893 12.0 1 8 0.83 +1Score > 19 indicates identity LLANGGNPEDVLAQLAR + 3 Deamidated (NQ)
1529 586.8380 1171.6614 1171.6713 -8.42 1 8 0.79 +1Score > 19 indicates identity EPRAFLAAVAK
2750 586.9974 1757.9704 1757.9424 15.9 1 8 0.43 +1Score > 16 indicates identity ALRIGQAYVDSAQLPR + Deamidated (NQ)
202 474.2695 473.2622 473.2598 5.13 0 8 0.64 +1Score > 29 indicates identity
Score > 18 indicates homology		 NVGGK
2268 769.8981 1537.7816 1537.7736 5.22 1 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 APEGLDDPLASVAQR
2000 469.9149 1406.7229 1406.7419 -13.5 1 7 0.96 +1Score > 20 indicates identity LGKVHQDIGHFR + Deamidated (NQ)
4240 960.4893 2878.4461 2878.4713 -8.75 0 7 0.62 +1Score > 18 indicates identity SGSADDATFLLSNADSVIIVPGYGLAVAR
2979 938.4531 1874.8916 1874.9149 -12.4 1 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 QIELDDVQFDLNALIE + Deamidated (NQ)
444 344.6880 687.3614 687.3551 9.17 0 7 0.64 +1Score > 23 indicates identity
Score > 18 indicates homology		 QSTQPK
1947 462.9200 1385.7382 1385.7337 3.25 1 7 0.23 +1Score > 21 indicates identity
Score > 13 indicates homology		 LGKLQLDDMTPR
2705 866.9415 1731.8684 1731.8899 -12.4 0 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 MIDGTVTVGPNAVLAMK + Oxidation (M)
214 488.3217 487.3144 487.3118 5.39 1 7 0.36 +1Score > 22 indicates identity
Score > 15 indicates homology		 KAAAK
813 428.7305 855.4464 855.4450 1.68 1 7 0.81 +1Score > 19 indicates identity ISQKDHK + Deamidated (NQ)
888 446.7436 891.4726 891.4814 -9.83 0 7 0.64 +1Score > 20 indicates identity
Score > 18 indicates homology		 QTQVFIR + Deamidated (NQ)
1525 391.2064 1170.5974 1170.5993 -1.62 1 7 0.29 +1Score > 21 indicates identity
Score > 14 indicates homology		 QAEALGIDAQR
1690 414.8913 1241.6521 1241.6364 12.6 1 7 0.33 +1Score > 21 indicates identity
Score > 15 indicates homology		 RALGLDTNPQR + 2 Deamidated (NQ)
3011 948.5011 1894.9876 1894.9822 2.86 1 7 0.98 +1Score > 19 indicates identity HSVPTIQEIVNLQMLR + 2 Deamidated (NQ); Oxidation (M)
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