MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3504 726.6904 2177.0494
3507 728.3957 2182.1653
3508 1092.5867 2183.1588
3509 546.7988 2183.1661
3510 728.7298 2183.1676
3512 730.3773 2188.1101
3517 731.3678 2191.0816
3519 731.3788 2191.1146
3520 731.3846 2191.1320
3521 1097.0638 2192.1130
3522 731.7117 2192.1133
3523 731.7120 2192.1142
3524 1097.0646 2192.1146
3525 731.7125 2192.1157
3526 731.7137 2192.1193
3527 731.7138 2192.1196
3528 731.7144 2192.1214
3534 733.0221 2196.0445
3542 550.3173 2197.2401
3543 734.7496 2201.2270
3546 735.3503 2203.0291
3547 1102.5265 2203.0384
3548 735.3549 2203.0429
3549 735.3581 2203.0525
3550 735.3824 2203.1254
3553 552.2914 2205.1365
3556 1105.0837 2208.1528
3557 737.0613 2208.1621
3558 1105.5762 2209.1378
3559 737.3873 2209.1401
3560 737.3882 2209.1428
3561 737.3892 2209.1458
3562 1106.5411 2211.0676
3563 738.0299 2211.0679
3564 738.0302 2211.0688
3565 738.6880 2213.0422
3567 739.3644 2215.0714
3568 1108.5664 2215.1182
3569 739.7435 2216.2087
3570 1109.5763 2217.1380
3574 1111.5991 2221.1836
3575 741.6849 2222.0329
3577 741.6859 2222.0359
3578 557.0527 2224.1817
3579 743.0604 2226.1594
3580 743.3718 2227.0936
3581 558.2545 2228.9889
3586 744.3757 2230.1053
3589 560.0353 2236.1121
3591 746.3849 2236.1329
3593 1119.5773 2237.1400
3594 746.7219 2237.1439
3595 747.0291 2238.0655
3596 747.4134 2239.2184
3597 1122.1067 2242.1988
3598 1122.1072 2242.1998
3599 1122.1073 2242.2000
3601 748.4100 2242.2082
3602 1122.1229 2242.2312
3603 748.4179 2242.2319
3604 748.4185 2242.2337
3605 748.4301 2242.2685
3612 1124.6361 2247.2576
3613 563.0341 2248.1073
3614 750.3839 2248.1299
3616 750.7111 2249.1115
3617 751.4256 2251.2550
3618 564.0347 2252.1097
3619 564.0635 2252.2249
3620 752.0375 2253.0907
3621 564.5301 2254.0913
3622 752.3773 2254.1101
3623 752.4067 2254.1983
3626 1128.6073 2255.2000
3627 753.7159 2258.1259
3628 1130.5540 2259.0934
3629 754.0661 2259.1765
3630 754.3655 2260.0747
3631 754.3755 2260.1047
3633 754.3949 2260.1629
3634 754.3954 2260.1644
3635 566.2905 2261.1329
3636 1131.5808 2261.1470
3637 755.7364 2264.1874
3638 755.7473 2264.2201
3643 1134.0732 2266.1318
3644 756.3846 2266.1320
3645 756.3853 2266.1341
3646 756.3856 2266.1350
3647 758.3785 2272.1137
3648 758.4045 2272.1917
3649 758.4070 2272.1992
3650 759.3497 2275.0273
3656 760.4042 2278.1908
3657 571.0462 2280.1557
3658 571.5375 2282.1209
3659 761.7550 2282.2432
3660 762.0994 2283.2764
3661 762.3887 2284.1443
3662 762.3912 2284.1518
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