MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3342 702.3801 2104.1185
3343 702.3864 2104.1374
3346 703.3497 2107.0273
3359 704.3744 2110.1014
3360 704.7094 2111.1064
3361 705.0580 2112.1522
3362 1057.5933 2113.1720
3364 1058.5341 2115.0536
3365 706.0295 2115.0667
3366 1058.5892 2115.1638
3367 706.0647 2115.1723
3368 529.8036 2115.1853
3369 1059.0338 2116.0530
3370 1059.0353 2116.0560
3376 706.3658 2116.0756
3377 706.3679 2116.0819
3378 706.7026 2117.0860
3379 530.2791 2117.0873
3383 707.7191 2120.1355
3384 1061.5188 2121.0230
3386 708.0725 2121.1957
3387 708.4030 2122.1872
3388 708.7264 2123.1574
3389 532.2663 2125.0361
3390 709.3641 2125.0705
3391 1063.5743 2125.1340
3392 709.3860 2125.1362
3393 709.7189 2126.1349
3394 709.7190 2126.1352
3395 1064.5618 2127.1090
3396 1064.5620 2127.1094
3397 710.0456 2127.1150
3398 710.0457 2127.1153
3399 710.3894 2128.1464
3400 710.4200 2128.2382
3402 711.6915 2132.0527
3403 1067.0366 2132.0586
3404 1067.5201 2133.0256
3413 1068.5619 2135.1092
3415 712.7116 2135.1130
3419 1070.0190 2138.0234
3420 1070.5624 2139.1102
3421 536.0192 2140.0477
3422 714.3981 2140.1725
3424 1071.5712 2141.1278
3425 714.7171 2141.1295
3426 715.0522 2142.1348
3427 715.0845 2142.2317
3429 715.6979 2144.0719
3430 715.7000 2144.0782
3431 537.0286 2144.0853
3435 716.0629 2145.1669
3436 1073.5919 2145.1692
3437 537.3015 2145.1769
3439 1074.0068 2145.9990
3440 716.3954 2146.1644
3441 716.7697 2147.2873
3442 1074.6541 2147.2936
3444 717.0151 2148.0235
3445 538.0142 2148.0277
3448 717.3895 2149.1467
3453 718.0432 2151.1078
3455 718.3803 2152.1191
3456 718.3843 2152.1311
3458 1077.1168 2152.2190
3459 1077.5717 2153.1288
3460 718.7206 2153.1400
3462 719.0233 2154.0481
3463 719.6849 2156.0329
3464 720.0630 2157.1672
3465 1079.8126 2157.6106
3466 720.3548 2158.0426
3467 720.3555 2158.0447
3468 1080.0563 2158.0980
3471 721.0480 2160.1222
3472 1081.0688 2160.1230
3474 1082.5667 2163.1188
3475 1083.0741 2164.1336
3478 722.6893 2165.0461
3479 722.6893 2165.0461
3480 1083.5740 2165.1334
3481 542.5384 2166.1245
3482 1084.5552 2167.0958
3483 723.3893 2167.1461
3486 1086.1504 2170.2862
3487 724.4368 2170.2886
3490 543.7999 2171.1705
3491 725.0237 2172.0493
3492 544.2697 2173.0497
3493 725.3613 2173.0621
3494 1087.5404 2173.0662
3495 1087.5427 2173.0708
3496 725.3649 2173.0729
3497 725.3687 2173.0843
3498 1087.5520 2173.0894
3499 1087.5540 2173.0934
3500 725.3983 2173.1731
3501 726.3937 2176.1593
3502 726.4125 2176.2157
3503 1089.5245 2177.0344
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