MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3195 1012.5212 2023.0278
3196 1012.5238 2023.0330
3197 1012.5245 2023.0344
3199 1012.5250 2023.0354
3200 1012.5261 2023.0376
3201 1012.5270 2023.0394
3202 1012.5275 2023.0404
3203 1012.5280 2023.0414
3204 1012.5283 2023.0420
3205 1012.5289 2023.0432
3206 1012.5338 2023.0530
3210 1016.5350 2031.0554
3212 678.3381 2031.9925
3213 678.3390 2031.9952
3216 678.6965 2033.0677
3217 679.0269 2034.0589
3218 1018.0381 2034.0616
3219 679.3580 2035.0522
3220 1018.5435 2035.0724
3221 679.3667 2035.0783
3222 679.3931 2035.1575
3223 1019.0373 2036.0600
3224 1019.5245 2037.0344
3226 680.3354 2037.9844
3227 680.3360 2037.9862
3229 680.3438 2038.0096
3232 680.6634 2038.9684
3233 1020.4936 2038.9726
3234 1020.4957 2038.9768
3235 1020.4979 2038.9812
3236 680.6786 2039.0140
3237 510.7723 2039.0601
3238 681.0325 2040.0757
3243 682.3720 2044.0942
3244 512.0315 2044.0969
3245 682.3758 2044.1056
3246 682.6606 2044.9600
3248 682.7134 2045.1184
3250 684.0004 2048.9794
3251 513.2529 2048.9825
3252 1026.5431 2051.0716
3253 685.3850 2053.1332
3254 1028.4977 2054.9808
3255 514.7542 2054.9877
3256 686.0111 2055.0115
3257 686.3355 2055.9847
3258 686.3360 2055.9862
3259 686.3741 2056.1005
3260 686.7150 2057.1232
3261 687.3449 2059.0129
3266 1030.5315 2059.0484
3268 1030.5336 2059.0526
3273 1031.0287 2060.0428
3274 687.7054 2060.0944
3275 1031.0939 2060.1732
3277 688.0388 2061.0946
3278 688.0389 2061.0949
3279 1037.1151 2072.2156
3280 1037.1250 2072.2354
3285 692.3809 2074.1209
3286 1038.5250 2075.0354
3287 692.6864 2075.0374
3288 1038.5287 2075.0428
3291 1038.5674 2075.1202
3293 693.3727 2077.0963
3294 520.2916 2077.1373
3295 520.2919 2077.1385
3296 693.4114 2077.2124
3297 520.3114 2077.2165
3298 693.7033 2078.0881
3299 1040.0530 2078.0914
3300 693.7076 2078.1010
3301 693.7206 2078.1400
3302 520.5427 2078.1417
3303 1041.0509 2080.0872
3304 1041.5090 2081.0034
3307 695.6880 2084.0422
3308 696.0094 2085.0064
3309 696.0140 2085.0202
3310 1043.5287 2085.0428
3312 1044.5095 2087.0044
3313 696.6758 2087.0056
3314 696.6760 2087.0062
3316 1044.5513 2087.0880
3317 696.7037 2087.0893
3318 696.7037 2087.0893
3321 1047.0332 2092.0518
3322 1047.0513 2092.0880
3323 698.3766 2092.1080
3324 1047.5403 2093.0660
3325 1047.5509 2093.0872
3326 699.3410 2095.0012
3330 699.6890 2096.0452
3332 700.0080 2097.0022
3333 1050.0485 2098.0824
3334 700.7034 2099.0884
3336 701.0439 2100.1099
3338 1051.5105 2101.0064
3340 1051.5236 2101.0326
3341 702.0527 2103.1363
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