MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3002 947.4944 1892.9742
3003 947.4961 1892.9776
3005 947.5014 1892.9882
3007 947.9993 1893.9840
3010 948.0170 1894.0194
3012 633.6646 1897.9720
3013 475.9765 1899.8769
3014 634.3531 1900.0375
3015 952.0333 1902.0520
3016 952.4910 1902.9674
3017 636.2797 1905.8173
3018 953.9199 1905.8252
3019 477.4638 1905.8261
3020 636.2828 1905.8266
3022 954.0017 1905.9888
3023 636.3428 1906.0066
3024 954.0720 1906.1294
3025 636.6166 1906.8280
3027 636.7188 1907.1346
3029 637.6671 1909.9795
3030 478.7525 1910.9809
3034 957.4987 1912.9828
3035 957.9791 1913.9436
3038 638.9912 1913.9518
3039 639.0262 1914.0568
3040 639.3276 1914.9610
3049 962.4586 1922.9026
3051 642.3169 1923.9289
3052 642.3206 1923.9400
3054 963.4803 1924.9460
3056 483.2450 1928.9509
3057 644.3259 1929.9559
3064 645.6590 1933.9552
3065 645.6933 1934.0581
3067 969.0457 1936.0768
3073 649.7079 1946.1019
3074 650.3768 1948.1086
3076 650.9945 1949.9617
3077 650.9963 1949.9671
3078 488.5084 1950.0045
3080 488.7493 1950.9681
3081 651.6855 1952.0347
3084 652.6967 1955.0683
3093 980.0229 1958.0312
3094 655.9913 1964.9521
3098 656.3791 1966.1155
3109 986.5027 1970.9908
3110 986.5309 1971.0472
3111 658.0397 1971.0973
3112 659.0160 1974.0262
3113 990.5023 1978.9900
3114 990.5306 1979.0466
3115 660.9783 1979.9131
3116 990.9639 1979.9132
3118 495.9885 1979.9249
3119 661.3265 1980.9577
3121 991.9875 1981.9604
3122 661.6613 1981.9621
3123 991.9895 1981.9644
3125 662.0543 1983.1411
3126 992.5800 1983.1454
3129 663.3448 1987.0126
3130 994.9758 1987.9370
3131 663.6540 1987.9402
3134 996.0161 1990.0176
3135 996.0193 1990.0240
3138 665.0341 1992.0805
3140 499.7463 1994.9561
3141 998.5534 1995.0922
3142 666.0635 1995.1687
3143 666.3253 1995.9541
3144 666.3771 1996.1095
3147 667.0024 1997.9854
3148 667.0031 1997.9875
3149 1000.0304 1998.0462
3150 667.0233 1998.0481
3151 1000.4853 1998.9560
3152 667.3284 1998.9634
3154 1001.5340 2001.0534
3155 668.0444 2001.1114
3157 1002.5440 2003.0734
3158 502.2603 2005.0121
3160 669.7263 2006.1571
3161 502.7669 2007.0385
3162 1004.5801 2007.1456
3163 1004.5831 2007.1516
3164 670.0583 2007.1531
3165 670.0615 2007.1627
3166 1005.5526 2009.0906
3169 1005.5583 2009.1020
3172 503.5081 2010.0033
3174 671.0348 2010.0826
3181 671.6833 2012.0281
3182 1008.5306 2015.0466
3184 1010.0072 2017.9998
3185 673.6747 2018.0023
3187 673.7211 2018.1415
3190 1010.5549 2019.0952
3193 674.6950 2021.0632
3194 1012.0228 2022.0310
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