MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2823 598.3003 1791.8791
2831 600.3364 1797.9874
2832 900.5039 1798.9932
2833 601.0164 1800.0274
2834 451.2072 1800.7997
2835 601.2740 1800.8002
2836 451.2079 1800.8025
2837 601.2758 1800.8056
2841 601.3297 1800.9673
2842 601.3509 1801.0309
2847 901.9920 1801.9694
2848 902.4133 1802.8120
2854 602.3160 1803.9262
2855 602.6391 1804.8955
2856 602.6407 1804.9003
2863 603.6454 1807.9144
2868 604.3557 1810.0453
2869 906.0317 1810.0488
2872 604.6699 1810.9879
2873 906.9393 1811.8640
2874 604.9629 1811.8669
2875 604.9995 1811.9767
2876 907.0084 1812.0022
2877 907.4578 1812.9010
2883 455.4702 1817.8517
2884 606.9581 1817.8525
2885 909.9903 1817.9660
2886 606.9970 1817.9692
2887 456.4897 1821.9297
2888 912.4656 1822.9166
2889 365.5972 1822.9496
2890 608.6575 1822.9507
2891 456.7450 1822.9509
2894 456.9959 1823.9545
2896 457.2394 1824.9285
2897 457.2449 1824.9505
2898 913.9448 1825.8750
2899 913.9562 1825.8978
2904 915.9256 1829.8366
2905 612.6594 1834.9564
2908 613.3459 1837.0159
2909 919.9460 1837.8774
2912 920.0046 1837.9946
2916 369.1916 1840.9216
2917 461.2399 1840.9305
2918 614.6522 1840.9348
2920 614.6919 1841.0539
2921 615.0159 1842.0259
2923 922.9487 1843.8828
2924 615.6369 1843.8889
2926 616.3235 1845.9487
2932 925.4485 1848.8824
2933 617.6552 1849.9438
2935 618.9712 1853.8918
2936 928.4478 1854.8810
2937 928.4523 1854.8900
2938 928.4540 1854.8934
2939 928.4562 1854.8978
2940 619.3267 1854.9583
2941 928.9542 1855.8938
2942 619.6395 1855.8967
2944 929.4973 1856.9800
2945 465.4628 1857.8221
2946 620.3201 1857.9385
2947 929.9774 1857.9402
2948 620.6554 1858.9444
2951 931.4745 1860.9344
2953 932.9931 1863.9716
2954 622.3400 1863.9982
2956 933.4648 1864.9150
2957 622.6657 1864.9753
2958 622.6663 1864.9771
2959 933.4964 1864.9782
2960 622.6768 1865.0086
2961 933.9272 1865.8398
2964 623.3414 1867.0024
2965 934.5584 1867.1022
2966 623.3757 1867.1053
2968 624.3480 1870.0222
2970 468.7636 1871.0253
2971 936.5234 1871.0322
2972 936.9677 1871.9208
2973 624.9843 1871.9311
2974 625.6856 1874.0350
2975 469.5162 1874.0357
2976 625.6912 1874.0518
2977 469.5205 1874.0529
2978 625.9709 1874.8909
2982 627.3192 1878.9358
2985 940.5048 1878.9950
2986 940.5060 1878.9974
2989 628.9685 1883.8837
2991 629.0074 1884.0004
2994 944.5060 1886.9974
2995 944.9387 1887.8628
2996 630.3397 1887.9973
2997 630.9890 1889.9452
2998 946.4820 1890.9494
3000 947.4865 1892.9584
3001 947.4927 1892.9708
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