MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2628 849.4337 1696.8528
2632 850.9760 1699.9374
2633 567.6549 1699.9429
2634 851.4214 1700.8282
2635 567.9522 1700.8348
2636 567.9526 1700.8360
2637 568.3321 1701.9745
2641 852.4709 1702.9272
2645 854.9288 1707.8430
2646 570.2884 1707.8434
2647 570.2894 1707.8464
2649 855.4301 1708.8456
2652 855.9615 1709.9084
2659 856.9965 1711.9784
2661 429.2143 1712.8281
2662 571.9909 1712.9509
2663 857.4882 1712.9618
2664 572.2838 1713.8296
2668 572.3165 1713.9277
2678 574.3007 1719.8803
2680 861.4655 1720.9164
2682 862.4720 1722.9294
2685 576.0095 1725.0067
2686 863.5115 1725.0084
2688 576.3416 1726.0030
2689 864.0107 1726.0068
2691 864.4684 1726.9222
2692 864.4730 1726.9314
2693 576.6642 1726.9708
2694 576.9563 1727.8471
2695 864.9667 1727.9188
2696 576.9871 1727.9395
2699 866.0107 1730.0068
2701 866.8888 1731.7630
2703 578.2625 1731.7657
2704 578.2627 1731.7663
2708 867.0291 1732.0436
2709 578.3572 1732.0498
2710 867.0338 1732.0530
2711 578.5963 1732.7671
2712 867.3918 1732.7690
2713 867.4758 1732.9370
2714 578.6542 1732.9408
2715 578.6557 1732.9453
2717 578.6650 1732.9732
2718 578.9669 1733.8789
2719 578.9873 1733.9401
2721 869.9695 1737.9244
2723 870.5100 1739.0054
2724 871.4103 1740.8060
2725 581.2983 1740.8731
2726 871.9930 1741.9714
2728 871.9961 1741.9776
2729 581.6686 1741.9840
2730 872.4312 1742.8478
2732 582.0013 1742.9821
2733 582.2904 1743.8494
2735 873.4523 1744.8900
2737 583.3145 1746.9217
2738 875.4686 1748.9226
2739 876.0055 1749.9964
2740 876.4324 1750.8502
2741 584.9785 1751.9137
2744 877.9588 1753.9030
2746 586.2486 1755.7240
2747 586.6010 1756.7812
2748 879.8996 1757.7846
2749 879.9918 1757.9690
2751 586.9986 1757.9740
2758 881.0338 1760.0530
2759 587.9512 1760.8318
2761 882.4688 1762.9230
2765 882.4899 1762.9652
2767 588.9937 1763.9593
2768 589.0004 1763.9794
2769 589.2958 1764.8656
2770 883.9402 1765.8658
2784 590.2921 1767.8545
2785 884.9376 1767.8606
2786 885.0067 1767.9988
2787 885.4474 1768.8802
2788 590.9116 1769.7130
2789 885.8638 1769.7130
2790 590.9610 1769.8612
2791 443.6861 1770.7153
2794 887.4426 1772.8706
2796 887.4528 1772.8910
2797 887.4550 1772.8954
2799 592.6525 1774.9357
2802 889.4603 1776.9060
2805 593.3193 1776.9361
2806 593.6108 1777.8106
2809 596.3239 1785.9499
2811 596.3297 1785.9673
2816 597.0279 1788.0619
2817 895.3993 1788.7840
2818 597.3112 1788.9118
2819 597.3120 1788.9142
2821 895.5427 1789.0708
2822 895.9604 1789.9062
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