MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2348 789.4216 1576.8286
2361 527.9578 1580.8516
2362 791.9148 1581.8150
2364 792.3981 1582.7816
2367 793.9031 1585.7916
2368 529.6275 1585.8607
2370 529.6300 1585.8682
2374 397.9667 1587.8377
2378 531.2798 1590.8176
2379 796.4164 1590.8182
2382 532.2674 1593.7804
2384 798.4603 1594.9060
2393 799.9545 1597.8944
2394 801.4277 1600.8408
2400 803.9254 1605.8362
2401 803.9275 1605.8404
2404 402.4806 1605.8933
2405 804.4260 1606.8374
2407 804.8945 1607.7744
2408 536.9327 1607.7763
2412 805.5066 1608.9986
2413 537.3407 1609.0003
2414 537.5947 1609.7623
2417 806.4319 1610.8492
2418 806.4360 1610.8574
2419 806.4450 1610.8754
2423 806.9328 1611.8510
2430 806.9443 1611.8740
2432 807.4431 1612.8716
2434 807.9266 1613.8386
2435 538.9540 1613.8402
2437 538.9556 1613.8450
2445 808.9418 1615.8690
2446 539.6306 1615.8700
2449 540.6334 1618.8784
2457 406.9666 1623.8373
2461 815.4659 1628.9172
2468 408.7238 1630.8661
2469 544.6305 1630.8697
2470 816.4521 1630.8896
2471 408.7465 1630.9569
2472 544.6597 1630.9573
2474 327.3990 1631.9586
2479 818.4020 1634.7894
2482 409.7281 1634.8833
2483 545.9688 1634.8846
2484 546.2481 1635.7225
2485 546.3295 1635.9667
2487 546.9587 1637.8543
2496 820.9165 1639.8184
2497 547.6324 1639.8754
2498 820.9457 1639.8768
2505 549.6499 1645.9279
2522 550.9617 1649.8633
2523 826.4331 1650.8516
2524 826.4353 1650.8560
2526 826.4458 1650.8770
2527 413.9629 1651.8225
2528 551.9501 1652.8285
2530 827.4528 1652.8910
2531 551.9776 1652.9110
2542 830.9286 1659.8426
2543 554.9668 1661.8786
2544 831.9503 1661.8860
2545 832.4410 1662.8674
2546 555.2968 1662.8686
2554 834.4680 1666.9214
2557 834.9461 1667.8776
2568 835.0080 1668.0014
2569 835.0170 1668.0194
2573 557.3345 1668.9817
2574 557.3348 1668.9826
2575 557.3361 1668.9865
2576 557.3365 1668.9877
2577 557.3368 1668.9886
2578 557.3387 1668.9943
2580 836.4341 1670.8536
2581 558.2838 1671.8296
2586 419.0092 1672.0077
2591 419.2599 1673.0105
2592 335.6094 1673.0106
2593 558.9841 1673.9305
2595 839.9553 1677.8960
2596 560.3179 1677.9319
2598 560.9648 1679.8726
2599 841.4560 1680.8974
2600 842.4494 1682.8842
2601 562.2921 1683.8545
2602 562.6334 1684.8784
2603 843.4778 1684.9410
2604 843.9453 1685.8760
2605 843.9462 1685.8778
2606 563.3008 1686.8806
2616 565.2864 1692.8374
2622 848.4898 1694.9650
2623 566.2882 1695.8428
2624 848.9288 1695.8430
2625 566.2904 1695.8494
2626 566.2963 1695.8671
2627 566.3093 1695.9061
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