MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 2947)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1997 703.9102 1405.8058
1998 704.3603 1406.7060
1999 704.3668 1406.7190
2008 706.9087 1411.8028
2009 707.3121 1412.6096
2019 474.2579 1419.7519
2020 710.8995 1419.7844
2022 711.8265 1421.6384
2023 474.8921 1421.6545
2026 711.9091 1421.8036
2028 712.3592 1422.7038
2029 712.3599 1422.7052
2030 712.3609 1422.7072
2035 475.5767 1423.7083
2037 475.5775 1423.7107
2039 712.8637 1423.7128
2040 713.1464 1424.2782
2042 714.4226 1426.8306
2056 717.8661 1433.7176
2062 481.2476 1440.7210
2069 722.3756 1442.7366
2070 722.4033 1442.7920
2072 481.9954 1442.9644
2076 483.6030 1447.7872
2081 726.8560 1451.6974
2084 728.9306 1455.8466
2086 729.9289 1457.8432
2092 730.4321 1458.8496
2093 730.4326 1458.8506
2094 487.6195 1459.8367
2096 366.1926 1460.7413
2097 732.3901 1462.7656
2100 733.3914 1464.7682
2101 489.6330 1465.8772
2110 735.4107 1468.8068
2111 490.9283 1469.7631
2131 492.9634 1475.8684
2132 492.9634 1475.8684
2133 739.3698 1476.7250
2134 493.2906 1476.8500
2136 493.6070 1477.7992
2143 495.0081 1482.0025
2145 742.4144 1482.8142
2157 746.4113 1490.8080
2158 746.8885 1491.7624
2160 498.6290 1492.8652
2168 748.8994 1495.7842
2172 499.9331 1496.7775
2174 749.9318 1497.8490
2175 500.2917 1497.8533
2176 749.9348 1497.8550
2177 749.9380 1497.8614
2180 500.6306 1498.8700
2181 750.4451 1498.8756
2182 750.4489 1498.8832
2183 500.6693 1498.9861
2184 750.9417 1499.8688
2185 751.4274 1500.8402
2203 752.4056 1502.7966
2204 752.4058 1502.7970
2205 752.4081 1502.8016
2212 754.9338 1507.8530
2214 755.3883 1508.7620
2215 755.3884 1508.7622
2218 378.7173 1510.8401
2219 756.6498 1511.2850
2221 504.9160 1511.7262
2225 379.2094 1512.8085
2227 505.2783 1512.8131
2232 758.9332 1515.8518
2239 760.4282 1518.8418
2243 763.4210 1524.8274
2258 511.6338 1531.8796
2259 766.9502 1531.8858
2260 383.9890 1531.9269
2262 512.5845 1534.7317
2267 769.3880 1536.7614
2273 514.6288 1540.8646
2278 772.4261 1542.8376
2283 774.8882 1547.7618
2287 387.9579 1547.8025
2290 775.9102 1549.8058
2295 777.4547 1552.8948
2299 519.2978 1554.8716
2300 778.8528 1555.6910
2301 519.6237 1555.8493
2303 779.9257 1557.8368
2306 780.8951 1559.7756
2308 520.9580 1559.8522
2318 783.9034 1565.7922
2319 783.9034 1565.7922
2320 392.4601 1565.8113
2321 523.2726 1566.7960
2322 523.2767 1566.8083
2326 784.9388 1567.8630
2330 524.2900 1569.8482
2334 525.3208 1572.9406
2335 787.4779 1572.9412
2336 787.4786 1572.9426
2337 787.9202 1573.8258
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