MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1554 591.3655 1180.7164
1555 591.3656 1180.7166
1557 394.9008 1181.6806
1566 594.3203 1186.6260
1569 595.2989 1188.5832
1572 595.8287 1189.6428
1573 596.3004 1190.5862
1575 596.7973 1191.5800
1577 597.8194 1193.6242
1591 600.0679 1198.1212
1596 601.3397 1200.6648
1610 405.2047 1212.5923
1612 607.3323 1212.6500
1614 607.3342 1212.6538
1617 405.8919 1214.6539
1618 405.8957 1214.6653
1629 611.3808 1220.7470
1630 611.3815 1220.7484
1631 611.3817 1220.7488
1638 408.5609 1222.6609
1650 410.5760 1228.7062
1651 410.5762 1228.7068
1652 410.5764 1228.7074
1654 410.8675 1229.5807
1656 615.9116 1229.8086
1670 618.3499 1234.6852
1672 619.3148 1236.6150
1673 619.7977 1237.5808
1684 620.7993 1239.5840
1685 620.8168 1239.6190
1688 414.2372 1239.6898
1691 415.5487 1243.6243
1701 627.8573 1253.7000
1712 421.5730 1261.6972
1713 421.8969 1262.6689
1734 638.3214 1274.6282
1737 426.2263 1275.6571
1743 320.1862 1276.7157
1744 426.5797 1276.7173
1747 639.8778 1277.7410
1748 640.3138 1278.6130
1751 427.5927 1279.7563
1752 640.8864 1279.7582
1754 427.8983 1280.6731
1767 430.8857 1289.6353
1769 430.9359 1289.7859
1774 431.9079 1292.7019
1780 647.9133 1293.8120
1782 649.3104 1296.6062
1785 434.2072 1299.5998
1788 434.5594 1300.6564
1804 657.2753 1312.5360
1814 439.8837 1316.6293
1818 440.5688 1318.6846
1823 660.8468 1319.6790
1826 331.1997 1320.7697
1834 443.2664 1326.7774
1844 334.4386 1333.7253
1857 672.3625 1342.7104
1858 448.8992 1343.6758
1861 673.3631 1344.7116
1862 449.8711 1346.5915
1864 450.5665 1348.6777
1868 675.8434 1349.6722
1872 451.9027 1352.6863
1878 678.3401 1354.6656
1886 454.2667 1359.7783
1890 455.2480 1362.7222
1892 455.2503 1362.7291
1894 682.8373 1363.6600
1899 683.8409 1365.6672
1900 456.2300 1365.6682
1901 456.2306 1365.6700
1914 684.3613 1366.7080
1921 457.9152 1370.7238
1925 458.8864 1373.6374
1926 458.8899 1373.6479
1936 460.6004 1378.7794
1939 461.9231 1382.7475
1944 693.3956 1384.7766
1945 693.8544 1385.6942
1948 693.8919 1385.7692
1953 694.3918 1386.7690
1954 694.8250 1387.6354
1958 694.8970 1387.7794
1960 695.3799 1388.7452
1961 463.9343 1388.7811
1963 463.9588 1388.8546
1970 465.8961 1394.6665
1973 467.2581 1398.7525
1974 467.2732 1398.7978
1978 700.4217 1398.8288
1985 351.9373 1403.7201
1986 351.9373 1403.7201
1987 351.9377 1403.7217
1988 468.9225 1403.7457
1989 468.9308 1403.7706
1990 703.3412 1404.6678
1991 703.8739 1405.7332
1993 703.8943 1405.7740
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