MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
862 880.9544 879.9471
872 443.2939 884.5732
886 446.7254 891.4362
907 450.2837 898.5528
910 450.2888 898.5630
920 903.4756 902.4683
921 903.4798 902.4725
926 906.4974 905.4901
928 906.5020 905.4947
930 907.4694 906.4621
932 455.3016 908.5886
933 912.4757 911.4684
934 912.4783 911.4710
938 912.8011 911.7938
939 457.2431 912.4716
965 923.1498 922.1425
967 924.4796 923.4723
969 463.2642 924.5138
972 927.4951 926.4878
987 466.2477 930.4808
1001 313.8354 938.4844
1003 939.9734 938.9661
1012 941.5213 940.5140
1016 314.8367 941.4883
1018 942.5053 941.4980
1033 947.4995 946.4922
1034 947.5005 946.4932
1040 316.8561 947.5465
1072 480.3279 958.6412
1078 481.7651 961.5156
1121 985.5085 984.5012
1139 332.8548 995.5426
1141 498.7800 995.5454
1146 333.1951 996.5635
1151 501.2494 1000.4842
1155 501.2961 1000.5776
1182 1015.6092 1014.6019
1183 339.4989 1015.4749
1190 509.7801 1017.5456
1196 341.5391 1021.5955
1197 342.8571 1025.5495
1198 343.1928 1026.5566
1199 343.1935 1026.5587
1200 343.1937 1026.5593
1203 514.8106 1027.6066
1206 344.5038 1030.4896
1215 1033.2139 1032.2066
1233 346.8705 1037.5897
1234 1040.5527 1039.5454
1252 1053.5184 1052.5111
1253 527.7217 1053.4288
1262 353.5281 1057.5625
1267 530.7456 1059.4766
1268 1060.5538 1059.5465
1278 356.2059 1065.5959
1281 356.5375 1066.5907
1283 357.1589 1068.4549
1284 535.2349 1068.4552
1298 1074.5527 1073.5454
1308 1078.5751 1077.5678
1315 541.7524 1081.4902
1322 362.5459 1084.6159
1323 362.5460 1084.6162
1327 543.8004 1085.5862
1334 546.8076 1091.6006
1350 550.3111 1098.6076
1357 551.3006 1100.5866
1369 369.8670 1106.5792
1378 557.2607 1112.5068
1404 1124.1279 1123.1206
1406 1125.1339 1124.1266
1407 1125.5730 1124.5657
1409 376.2018 1125.5836
1415 377.2006 1128.5800
1419 377.5097 1129.5073
1429 378.1978 1131.5716
1430 566.7999 1131.5852
1436 378.8709 1133.5909
1437 378.8712 1133.5918
1443 569.2581 1136.5016
1444 379.8904 1136.6494
1445 569.3566 1136.6986
1446 380.2363 1137.6871
1454 571.8588 1141.7030
1455 571.8614 1141.7082
1456 571.8615 1141.7084
1458 572.8588 1143.7030
1463 573.8303 1145.6460
1464 382.9259 1145.7559
1470 575.8069 1149.5992
1475 576.8051 1151.5956
1491 386.5325 1156.5757
1493 579.3239 1156.6332
1494 579.7747 1157.5348
1505 580.8223 1159.6300
1524 391.2044 1170.5914
1540 1176.6200 1175.6127
1543 589.2989 1176.5832
1544 589.3009 1176.5872
1553 591.3651 1180.7156
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