MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 2947)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
201 473.3104 472.3031 472.3009 4.64 0 23 0.069 +1Score > 24 indicates identity AGVVK
442 687.3805 686.3732 686.3711 3.08 0 22 0.073 +1Score > 24 indicates identity GQAALAR + Deamidated (NQ)
242 531.3263 530.3190 530.3177 2.58 0 22 0.14 +1Score > 26 indicates identity VVTGR
1032 474.2393 946.4640 946.4616 2.61 1 22 0.054 +1Score > 22 indicates identity AMPEVAMAK
304 603.3364 602.3291 602.3275 2.65 1 21 0.14 +1Score > 25 indicates identity QVEVK + Deamidated (NQ)
616 388.2144 774.4142 774.4236 -12.0 1 21 0.074 +1Score > 23 indicates identity AISVTER
585 380.2203 758.4260 758.4287 -3.45 0 21 0.11 +1Score > 24 indicates identity VGAAVGTGK
740 415.7420 829.4694 829.4657 4.47 1 21 0.11 +1Score > 24 indicates identity QLIEATR
237 529.3471 528.3398 528.3384 2.76 0 20 0.11 +1Score > 23 indicates identity AVIAR
303 301.6863 601.3580 601.3548 5.48 0 20 0.13 +1Score > 24 indicates identity LSGAVR
1648 614.8381 1227.6616 1227.6710 -7.65 1 20 0.069 +1Score > 21 indicates identity DLLGQGAEAILK + Deamidated (NQ)
300 602.3524 601.3451 601.3435 2.72 1 20 0.15 +1Score > 24 indicates identity AEALAK
1247 524.2585 1046.5024 1046.4992 3.08 1 20 0.079 +1Score > 21 indicates identity LAQESGTANR + Deamidated (NQ)
410 336.7115 671.4084 671.3966 17.7 0 20 0.16 +1Score > 24 indicates identity AALQLR + Deamidated (NQ)
411 336.7121 671.4096 671.3966 19.4 0 19 0.17 +1Score > 24 indicates identity AALQLR + Deamidated (NQ)
322 308.6876 615.3606 615.3592 2.42 1 19 0.17 +1Score > 24 indicates identity AGELVK
1647 410.2278 1227.6616 1227.6758 -11.5 0 18 0.086 +1Score > 21 indicates identity
Score > 20 indicates homology		 MNVQNAILGLR
367 645.3320 644.3247 644.3242 0.88 1 18 0.11 +1Score > 21 indicates identity AQEAAR
276 585.3362 584.3289 584.3282 1.26 0 18 0.063 +1Score > 19 indicates identity QAIPR + Deamidated (NQ)
1564 593.8225 1185.6304 1185.6354 -4.14 0 18 0.14 +1Score > 22 indicates identity GVDVLIADTAGR
279 586.3684 585.3611 585.3598 2.21 0 18 0.14 +1Score > 22 indicates identity AVAGIR
2328 523.9535 1568.8387 1568.8344 2.71 1 17 0.1 +1Score > 20 indicates identity ASKLMHITQPALSR + Deamidated (NQ); Oxidation (M)
387 330.2089 658.4032 658.4014 2.86 0 17 0.081 +1Score > 19 indicates identity QLTVAK
704 407.7516 813.4886 813.4960 -9.00 1 17 0.094 +1Score > 20 indicates identity LIEGLAAK
1965 695.8746 1389.7346 1389.7220 9.07 1 17 0.1 +1Score > 20 indicates identity QMVAMRLLAQGR + Deamidated (NQ); Oxidation (M)
1919 685.8911 1369.7676 1369.7565 8.12 1 17 0.087 +1Score > 19 indicates identity AGVLEQGTVDLLR
640 392.7326 783.4506 783.4491 2.02 0 17 0.071 +1Score > 18 indicates identity GDVPGLVK
445 688.3691 687.3618 687.3551 9.71 0 16 0.28 +1Score > 23 indicates identity DGAQGLK
633 779.3958 778.3885 778.3861 3.10 1 16 0.18 +1Score > 21 indicates identity QFDELK
591 761.4062 760.3989 760.3967 2.99 0 16 0.23 +1Score > 22 indicates identity SDNLALK + Deamidated (NQ)
415 337.2037 672.3928 672.3919 1.47 1 16 0.28 +1Score > 23 indicates identity GLSIRGA
1027 472.7719 943.5292 943.5199 9.89 1 16 0.19 +1Score > 21 indicates identity TLTPSNRR
227 516.2921 515.2848 515.2816 6.31 0 16 0.22 +1Score > 22 indicates identity QAAAR
565 745.4592 744.4519 744.4494 3.43 1 16 0.17 +1Score > 20 indicates identity KQVLTR + Deamidated (NQ)
874 443.7667 885.5188 885.5032 17.7 1 15 0.17 +1Score > 20 indicates identity AQQLRLR + 2 Deamidated (NQ)
2305 780.4211 1558.8276 1558.8575 -19.1 1 15 0.19 +1Score > 21 indicates identity LKMLADGINIMLAR + Deamidated (NQ)
617 775.4332 774.4259 774.4123 17.6 0 15 0.25 +1Score > 22 indicates identity AIDQVTK + Deamidated (NQ)
1280 534.2745 1066.5344 1066.5481 -12.8 1 15 0.079 +1Score > 20 indicates identity
Score > 17 indicates homology		 EAYTALMLR
316 307.7089 613.4032 613.3911 19.8 0 15 0.2 +1Score > 21 indicates identity AALLAR
1168 503.2983 1004.5820 1004.5767 5.33 1 15 0.09 +1Score > 17 indicates identity TLARQFLR + Deamidated (NQ)
1875 452.2384 1353.6934 1353.6677 18.9 0 15 0.06 +1Score > 20 indicates identity
Score > 16 indicates homology		 QVQGLASFDFSR
1583 399.8902 1196.6488 1196.6401 7.28 1 15 0.14 +1Score > 19 indicates identity LLLNQQNPEK + Deamidated (NQ)
1584 599.3324 1196.6502 1196.6401 8.51 1 15 0.14 +1Score > 19 indicates identity LLLNQQNPEK + Deamidated (NQ)
1154 501.2867 1000.5588 1000.5665 -7.66 1 15 0.23 +1Score > 21 indicates identity KLADSQALR
1643 614.8373 1227.6600 1227.6710 -8.95 1 15 0.23 +1Score > 21 indicates identity DLLGQGAEAILK + Deamidated (NQ)
350 632.3749 631.3676 631.3765 -14.1 1 15 0.17 +1Score > 20 indicates identity SRITR
405 336.2198 670.4250 670.4126 18.6 0 15 0.25 +1Score > 21 indicates identity AGLAIAR
592 381.2077 760.4008 760.4079 -9.25 1 14 0.12 +1Score > 23 indicates identity
Score > 18 indicates homology		 AQSKAQK + Deamidated (NQ)
1379 557.8100 1113.6054 1113.6030 2.24 1 14 0.17 +1Score > 21 indicates identity
Score > 19 indicates homology		 LAEAQVLNQK + Deamidated (NQ)
649 394.7357 787.4568 787.4552 2.13 1 14 0.21 +1Score > 20 indicates identity ATAQRLK + Deamidated (NQ)
741 415.7469 829.4792 829.4770 2.74 1 14 0.43 +1Score > 23 indicates identity RLSDAIR
1425 1131.5677 1130.5604 1130.5819 -19.0 0 14 0.36 +1Score > 22 indicates identity TPLQSAGQLSK + 2 Deamidated (NQ)
775 423.2289 844.4432 844.4290 16.9 1 14 0.4 +1Score > 23 indicates identity
Score > 23 indicates homology		 QIIAEDR + Deamidated (NQ)
2810 893.9837 1785.9528 1785.9658 -7.27 1 14 0.17 +1Score > 19 indicates identity QLALICRDLQLTVNK + 2 Deamidated (NQ)
296 601.3311 600.3238 600.3344 -17.5 0 14 0.3 +1Score > 21 indicates identity AGGQLR
1218 345.5024 1033.4854 1033.4829 2.43 0 14 0.3 +1Score > 21 indicates identity GSFDAPNGLR + Deamidated (NQ)
236 529.3123 528.3050 528.3020 5.75 0 14 0.11 +1Score > 17 indicates identity APVSR
1348 549.3141 1096.6136 1096.6029 9.78 0 14 0.16 +1Score > 18 indicates identity IDIPSFIHR
1473 576.7715 1151.5284 1151.5095 16.5 0 14 0.06 +1Score > 20 indicates identity
Score > 14 indicates homology		 TNDTFGALNAE
1347 549.3129 1096.6112 1096.6029 7.59 0 14 0.15 +1Score > 18 indicates identity IDIPSFIHR
381 656.4137 655.4064 655.4017 7.23 0 14 0.067 +1Score > 14 indicates identity SIAPLR
743 416.2467 830.4788 830.4749 4.74 1 14 0.23 +1Score > 20 indicates identity QTLEVLK + Deamidated (NQ)
1565 593.8251 1185.6356 1185.6354 0.24 0 13 0.34 +1Score > 21 indicates identity
Score > 21 indicates homology		 GVDVLIADTAGR
3823 799.4138 2395.2196 2395.2019 7.36 0 13 0.22 +1Score > 19 indicates identity TYQGYAGDSLAAALLANGVDIVGR + Deamidated (NQ)
2509 413.4571 1649.7993 1649.7872 7.35 0 13 0.31 +1Score > 21 indicates identity FSMLLGYTQASFNR + Oxidation (M)
2309 780.9390 1559.8634 1559.8559 4.84 0 13 0.12 +1Score > 17 indicates identity SGYLLPLDVSLDIR
763 421.7237 841.4328 841.4294 4.15 1 13 0.23 +1Score > 19 indicates identity GQEAPALR + Deamidated (NQ)
788 425.7198 849.4250 849.4232 2.17 1 13 0.22 +1Score > 22 indicates identity
Score > 19 indicates homology		 LFAEQNK + Deamidated (NQ)
1888 682.3266 1362.6386 1362.6415 -2.12 0 13 0.11 +1Score > 21 indicates identity
Score > 16 indicates homology		 WSGSLVSQNLDR + 2 Deamidated (NQ)
1340 365.5255 1093.5547 1093.5404 13.1 1 13 0.1 +1Score > 20 indicates identity
Score > 16 indicates homology		 DYASLAELGR
264 569.3057 568.2984 568.2969 2.70 0 13 0.13 +1Score > 17 indicates identity NHVAK + Deamidated (NQ)
1542 589.2950 1176.5754 1176.5557 16.8 1 13 0.082 +1Score > 21 indicates identity
Score > 15 indicates homology		 AIAGMQRNATE + Oxidation (M)
1756 642.8351 1283.6556 1283.6357 15.5 1 13 0.28 +1Score > 20 indicates identity AELGADLEPVDR
3609 562.0563 2244.1961 2244.1579 17.0 1 13 0.14 +1Score > 17 indicates identity AFELPPLPYAHDALQPHISK + Deamidated (NQ)
747 417.2495 832.4844 832.4807 4.52 1 13 0.14 +1Score > 17 indicates identity LFGEVLR
1058 319.5149 955.5229 955.5273 -4.65 0 13 0.15 +1Score > 17 indicates identity ALAAPCVVR
1140 498.7792 995.5438 995.5512 -7.39 1 13 0.14 +1Score > 17 indicates identity LSALKHGDR
582 379.2332 756.4518 756.4494 3.25 1 13 0.43 +1Score > 22 indicates identity
Score > 21 indicates homology		 KGGPGITK
746 417.2328 832.4510 832.4477 4.07 0 12 0.31 +1Score > 20 indicates identity LVQGMLR + Deamidated (NQ); Oxidation (M)
2476 545.6111 1633.8115 1633.8233 -7.22 0 12 0.41 -1Score > 21 indicates identity LVNVTMTDVQNLIR + 3 Deamidated (NQ); Oxidation (M)
348 315.7138 629.4130 629.4112 2.97 1 12 0.13 +1Score > 16 indicates identity IKQLK + Deamidated (NQ)
601 383.6913 765.3680 765.3769 -11.6 0 12 0.17 +1Score > 21 indicates identity
Score > 17 indicates homology		 LDHPQR + Deamidated (NQ)
1123 494.7581 987.5016 987.5171 -15.7 0 12 0.53 +1Score > 22 indicates identity AGMLTNPLR + Oxidation (M)
1918 457.5961 1369.7665 1369.7453 15.5 1 12 0.26 +1Score > 19 indicates identity GEATLAPSLDLVGK
1995 469.5993 1405.7761 1405.7565 13.9 1 12 0.13 +1Score > 16 indicates identity LEPALHASVAAKAE
1979 467.6174 1399.8304 1399.8075 16.3 0 12 0.059 +1Score > 13 indicates identity IAIGVVQGLAFALE
2264 768.8712 1535.7278 1535.7548 -17.6 1 12 0.078 +1Score > 21 indicates identity
Score > 14 indicates homology		 LGMSQPMASASLRR + 2 Oxidation (M)
226 515.3321 514.3248 514.3227 4.08 0 12 0.77 +1Score > 26 indicates identity
Score > 23 indicates homology		 AGLVR
1083 482.7522 963.4898 963.5025 -13.1 1 12 0.32 +1Score > 20 indicates identity
Score > 20 indicates homology		 ELANVVYR + Deamidated (NQ)
1113 327.1916 978.5530 978.5433 9.90 1 12 0.22 +1Score > 18 indicates identity AFRAIMVR + Oxidation (M)
1714 632.8160 1263.6174 1263.6208 -2.62 0 12 0.48 +1Score > 21 indicates identity NTIGGAGADYVAR
299 602.3522 601.3449 601.3547 -16.3 0 12 0.88 +1Score > 24 indicates identity
Score > 24 indicates homology		 ALTAAR
887 892.4795 891.4722 891.4814 -10.3 1 12 0.32 +1Score > 20 indicates identity
Score > 20 indicates homology		 SVAEAFLR
1563 593.8217 1185.6288 1185.6241 3.99 0 12 0.53 +1Score > 22 indicates identity GVDVILNSLAGE
1261 353.5280 1057.5622 1057.5556 6.20 0 12 0.26 +1Score > 22 indicates identity
Score > 18 indicates homology		 QWILNDIR + Deamidated (NQ)
200 473.3098 472.3025 472.3009 3.43 0 12 0.19 +1Score > 17 indicates identity AIAAK
2387 799.4078 1596.8010 1596.8246 -14.8 0 12 0.27 +1Score > 21 indicates identity
Score > 19 indicates homology		 ADVSLLLNGDPLLQE + Deamidated (NQ)
991 932.4822 931.4749 931.4610 14.9 1 12 0.099 +1Score > 22 indicates identity
Score > 14 indicates homology		 DLESQAIR + Deamidated (NQ)
679 400.7429 799.4712 799.4664 6.06 1 12 0.39 +1Score > 20 indicates identity
Score > 20 indicates homology		 RQNLLR + Deamidated (NQ)
238 529.3472 528.3399 528.3384 2.95 0 12 0.79 +1Score > 23 indicates identity IIAGR
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