MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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91| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DD6_PSEPK | 155 | Protein HflC OS=Pseudomonas putida (strain KT2440) GN=hflC PE=3 SV=1 |
92| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPK | 149 | Alanine--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1 |
93| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NM0_PSEPK | 148 | Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=phoQ PE=4 SV=1 |
94| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL10_PSEPK | 146 | 50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1 |
95| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KZ0_PSEPK | 143 | Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=gap-2 PE=3 SV=1 |
96| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEPK | 142 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 96.1 | STHA_PSEPK |
142 | 51159 | 10 (6) | 7 (5) | 0.30 | |
| Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3 | |||||||
10 peptide matches (9 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 307 |
604.3674 | 603.3601 | 603.3592 | 1.60 | 0 | 22 | 0.016 | 2Score > 16 indicates identity |
U | E.ISSIGK.N | |
| 308 |
302.6877 | 603.3608 | 603.3592 | 2.80 | 0 | 21 | 0.022 | 2Score > 16 indicates identity |
U | E.ISSIGK.N | |
| 1062 |
478.7829 | 955.5512 | 955.5702 | -19.8 | 1 | 15 | 0.17 | 2Score > 20 indicates identity |
U | K.LGLENIGIK.V | |
| 1996 |
469.6075 | 1405.8007 | 1405.7929 | 5.52 | 1 | 38 | 0.00015 | 1Score > 13 indicates identity |
U | R.VEGLDNGVILHLK.S | |
| 2638 |
852.4312 | 1702.8478 | 1702.8526 | -2.80 | 0 | 27 | 0.0038 | 1Score > 21 indicates identity Score > 15 indicates homology |
U | R.VYDSDTILSLSHTPR.K | |
| 2640 |
568.6240 | 1702.8502 | 1702.8526 | -1.43 | 0 | 17 | 0.13 | 1Score > 21 indicates identity |
U | R.VYDSDTILSLSHTPR.K | |
| 2677 |
574.2905 | 1719.8497 | 1719.8284 | 12.4 | 1 | 5 | 0.92 | 1Score > 21 indicates identity Score > 17 indicates homology |
U | K.SMARAQIAGEPQGMLK.I + Deamidated (NQ); 2 Oxidation (M) | |
| 3450 |
1 |
1076.0746 | 2150.1346 | 2150.1147 | 9.28 | 1 | 27 | 0.0058 | 1Score > 17 indicates identity |
U | R.FVNDVPTGIYTIPEISSIGK.N + Deamidated (NQ) |
| 4135 |
918.8009 | 2753.3809 | 2753.3409 | 14.5 | 0 | 86 | 8.4e-009 | 1Score > 18 indicates identity |
U | R.TTVPNIYGAGDVIGWPSLASAAHDQGR.S + Deamidated (NQ) |
97| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS18_PSEPK | 141 | 30S ribosomal protein S18 OS=Pseudomonas putida (strain KT2440) GN=rpsR PE=3 SV=1 |
98| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88C30_PSEPK | 140 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5353 PE=4 SV=1 |
99| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L55_PSEPK | 138 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2080 PE=4 SV=1 |
100| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N36_PSEPK | 138 | 3-oxoadipate enol-lactone hydrolase OS=Pseudomonas putida (strain KT2440) GN=pcaD PE=4 SV=1 |
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