MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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91| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DD6_PSEPK | 155 | Protein HflC OS=Pseudomonas putida (strain KT2440) GN=hflC PE=3 SV=1 |
92| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPK | 149 | Alanine--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1 |
93| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NM0_PSEPK | 148 | Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=phoQ PE=4 SV=1 |
94| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL10_PSEPK | 146 | 50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1 |
95| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KZ0_PSEPK | 143 | Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=gap-2 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 95.1 | Q88KZ0_PSEPK |
143 | 53210 | 8 (5) | 6 (5) | 0.23 | |
| Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=gap-2 PE=3 SV=1 | |||||||
8 peptide matches (8 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 589 |
380.2542 | 758.4938 | 758.4902 | 4.85 | 0 | 13 | 0.045 | 1Score > 13 indicates identity |
U | R.SVISILK.A | |
| 789 |
425.7253 | 849.4360 | 849.4345 | 1.86 | 1 | 3 | 4.3 | 5Score > 22 indicates identity |
U | R.TAEQFVR.E | |
| 792 |
851.4180 | 850.4107 | 850.4185 | -9.11 | 1 | 15 | 0.18 | 1Score > 21 indicates identity Score > 20 indicates homology |
U | R.TAEQFVR.E + Deamidated (NQ) | |
| 793 |
426.2128 | 850.4110 | 850.4185 | -8.73 | 1 | 1 | 1.4 | 2Score > 21 indicates identity Score > 15 indicates homology |
U | R.TAEQFVR.E + Deamidated (NQ) | |
| 985 |
465.7681 | 929.5216 | 929.5182 | 3.72 | 0 | 49 | 8.2e-005 | 1Score > 21 indicates identity Score > 20 indicates homology |
U | K.LGAASVDLGK.L | |
| 3335 |
1051.0123 | 2100.0100 | 2100.0011 | 4.24 | 1 | 31 | 0.0037 | 5Score > 19 indicates identity |
U | K.QIDYVASQEVVSTDFVGSR.H + Deamidated (NQ) | |
| 4189 |
938.4608 | 2812.3606 | 2812.3192 | 14.7 | 1 | 72 | 2.6e-007 | 1Score > 19 indicates identity |
U | K.SPSEVDYTQYGIDNALIVDNTGVWR.D + Deamidated (NQ) | |
| 4634 |
1168.5923 | 3502.7551 | 3502.7104 | 12.8 | 1 | 47 | 4.5e-005 | 1Score > 16 indicates identity |
U | R.DSVHGPFDGTITIDEANNTITANGNLIQVIYAK.S + 2 Deamidated (NQ) |
96| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEPK | 142 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3 |
97| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS18_PSEPK | 141 | 30S ribosomal protein S18 OS=Pseudomonas putida (strain KT2440) GN=rpsR PE=3 SV=1 |
98| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88C30_PSEPK | 140 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5353 PE=4 SV=1 |
99| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L55_PSEPK | 138 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2080 PE=4 SV=1 |
100| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N36_PSEPK | 138 | 3-oxoadipate enol-lactone hydrolase OS=Pseudomonas putida (strain KT2440) GN=pcaD PE=4 SV=1 |
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