MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 313)

Page: Previous 1 …5 6 7 8 9 10 11 12 13 14 15 … 32 Next 

+91

Accession Score Description
1 Q88DD6_PSEPK 155 Protein HflC OS=Pseudomonas putida (strain KT2440) GN=hflC PE=3 SV=1

+92

Accession Score Description
1 SYA_PSEPK 149 Alanine--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1

+93

Accession Score Description
1 Q88NM0_PSEPK 148 Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=phoQ PE=4 SV=1

-94

Accession Score Description
1 RL10_PSEPK 146 50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1
Score Mass Matches Sequences emPAI
94.1 RL10_PSEPK 146 17676 8 (6) 6 (5) 0.87
50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1

-8 peptide matches (6 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
309   303.1460 604.2774 604.2704 11.6 1 5 1.8 +4Score > 23 indicates identity
Score > 20 indicates homology		 U R.AVEGTE.F
366   322.7097 643.4048 643.4017 4.91 0 26 0.02 +1Score > 22 indicates identity U R.TLAAIR.D
1292 +2 536.3140 1070.6134 1070.6084 4.72 0 63 3.1e-006 +1Score > 21 indicates identity U K.VALSAVVADAR.G
1438   567.8111 1133.6076 1133.6114 -3.34 0 32 0.0036 +1Score > 20 indicates identity U R.LMSVIQGATSK.L
2648   570.2997 1707.8773 1707.8692 4.70 1 67 1.2e-006 +1Score > 20 indicates identity U K.GPTLIAFSNEHPGAAAR.L
3819   797.4323 2389.2751 2389.2278 19.8 0 26 0.0062 +1Score > 16 indicates identity U K.AAAFDGNFIAANQIDVLATLPTR.D + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q88CJ4_PSEPK 26 52386 94.1
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5187 PE=4 SV=1

+95

Accession Score Description
1 Q88KZ0_PSEPK 143 Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=gap-2 PE=3 SV=1

+96

Accession Score Description
1 STHA_PSEPK 142 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3

+97

Accession Score Description
1 RS18_PSEPK 141 30S ribosomal protein S18 OS=Pseudomonas putida (strain KT2440) GN=rpsR PE=3 SV=1

+98

Accession Score Description
1 Q88C30_PSEPK 140 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5353 PE=4 SV=1

+99

Accession Score Description
1 Q88L55_PSEPK 138 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2080 PE=4 SV=1

+100

Accession Score Description
1 Q88N36_PSEPK 138 3-oxoadipate enol-lactone hydrolase OS=Pseudomonas putida (strain KT2440) GN=pcaD PE=4 SV=1
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