MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 91–100 (out of 313)


Page: Previous 1 5 6 7 8 9 10 11 12 13 14 15  32 Next 

-91

Accession Score Description
1 Q88DD6_PSEPK 155 Protein HflC OS=Pseudomonas putida (strain KT2440) GN=hflC PE=3 SV=1
Score Mass Matches Sequences emPAI
91.1 Q88DD6_PSEPK 155 32981 9 (6) 9 (6) 0.50
Protein HflC OS=Pseudomonas putida (strain KT2440) GN=hflC PE=3 SV=1

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
380   328.7031 655.3916 655.3905 1.82 0 31 0.0021 +1Score > 17 indicates identity U K.AIDLPK.E
493   356.6752 711.3358 711.3340 2.58 1 16 0.08 +1Score > 18 indicates identity K.NEFFR.Y
553   369.2024 736.3902 736.3868 4.73 0 12 0.33 +1Score > 19 indicates identity U R.SLQAYR.E
572   375.7247 749.4348 749.4323 3.36 1 25 0.0028 +1Score > 13 indicates identity U R.FLTLEK.K
1124   494.7852 987.5558 987.5502 5.76 0 60 5.2e-006 +1Score > 19 indicates identity U K.IPYVNQVR.R
1460   573.3052 1144.5958 1144.5910 4.21 0 50 8e-005 +1Score > 22 indicates identity U R.LMTLDAPTQR.F
1962   463.9352 1388.7838 1388.7776 4.43 0 19 0.027 +1Score > 16 indicates identity U K.VVQADVQPGLHVK.I
2282   774.3989 1546.7832 1546.7773 3.85 1 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology
U E.RDALMADITASLNR.M + Deamidated (NQ)
2376   530.9055 1589.6947 1589.6899 3.00 0 57 7.1e-006 +1Score > 18 indicates identity U K.AYSQDADFYAFHR.S

+92

Accession Score Description
1 SYA_PSEPK 149 Alanine--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1

+93

Accession Score Description
1 Q88NM0_PSEPK 148 Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=phoQ PE=4 SV=1

+94

Accession Score Description
1 RL10_PSEPK 146 50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1

+95

Accession Score Description
1 Q88KZ0_PSEPK 143 Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas putida (strain KT2440) GN=gap-2 PE=3 SV=1

+96

Accession Score Description
1 STHA_PSEPK 142 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3

+97

Accession Score Description
1 RS18_PSEPK 141 30S ribosomal protein S18 OS=Pseudomonas putida (strain KT2440) GN=rpsR PE=3 SV=1

+98

Accession Score Description
1 Q88C30_PSEPK 140 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5353 PE=4 SV=1

+99

Accession Score Description
1 Q88L55_PSEPK 138 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2080 PE=4 SV=1

+100

Accession Score Description
1 Q88N36_PSEPK 138 3-oxoadipate enol-lactone hydrolase OS=Pseudomonas putida (strain KT2440) GN=pcaD PE=4 SV=1
Page: Previous 1 5 6 7 8 9 10 11 12 13 14 15  32 Next 

Not what you expected? Try the select summary.