MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 313)

Page: 1 2 3 4 5 6 … 32 Next 

+1

Accession Score Description
1 Q88NM2_PSEPK 4307 Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 2609 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
2 EFTU1_PSEPK 2412 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1

+3

Accession Score Description
1 ATPB_PSEPK 2344 ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1

+4

Accession Score Description
1 Q88ES5_PSEPK 2322 Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1

+5

Accession Score Description
1 Q88L46_PSEPK 1745 Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1

+6

Accession Score Description
1 ATPA_PSEPK 1711 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1

+7

Accession Score Description
1 Q88FB0_PSEPK 1295 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1

+8

Accession Score Description
1 Q88FA7_PSEPK 1233 Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1

-9

Accession Score Description
1 RPOB_PSEPK 843 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1
Score Mass Matches Sequences emPAI
9.1 RPOB_PSEPK 843 151468 37 (29) 32 (27) 0.54
DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

-37 peptide matches (34 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
228 +1 516.3041 515.2968 515.2955 2.60 0 10 0.5 +3Score > 28 indicates identity
Score > 20 indicates homology		 R.ALAIE.K
364   322.7032 643.3918 643.3904 2.19 1 22 0.07 +3Score > 22 indicates identity U R.ALAIEK.M
424   339.6830 677.3514 677.3497 2.62 1 32 0.0053 +1Score > 22 indicates identity U R.AGFEVR.D
504   358.7194 715.4242 715.4228 1.97 0 37 0.0014 +1Score > 21 indicates identity U K.TLVDIR.N
515   361.7141 721.4136 721.4122 1.94 0 21 0.02 +1Score > 17 indicates identity U K.LLYSAR.I
544   367.7330 733.4514 733.4486 3.83 0 29 0.0013 +1Score > 13 indicates identity U K.VYLAIR.R
642 +1 392.7388 783.4630 783.4603 3.53 0 33 0.0014 +1Score > 16 indicates identity U R.QAVPTLR.A
678   400.7366 799.4586 799.4552 4.31 1 16 0.062 +1Score > 23 indicates identity
Score > 17 indicates homology		 U K.VIVEQGR.R
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 18 indicates identity
863   441.2482 880.4818 880.4766 5.92 1 22 0.021 -1Score > 18 indicates identity U R.HINQLEK.A
No other peptide matches in query
1048   475.7526 949.4906 949.4869 3.97 1 35 0.0014 +1Score > 21 indicates identity
Score > 18 indicates homology		 U R.EFLQAGASK.D
1054   317.8635 950.5687 950.5661 2.66 0 33 0.00073 +1Score > 14 indicates identity U R.VIVSQLHR.S
1055   318.5075 952.5007 952.4978 3.03 0 21 0.034 +1Score > 19 indicates identity U K.LNHLVDDK.M
1071   480.2906 958.5666 958.5600 6.92 0 35 0.00076 +1Score > 16 indicates identity U R.GVTFAVPLR.V
1130   496.7619 991.5092 991.5087 0.57 0 25 0.024 +1Score > 21 indicates identity U K.AQQYIVDR.R
1202   514.3008 1026.5870 1026.5822 4.72 0 53 2.1e-005 +1Score > 19 indicates identity U R.VSALGPGGLTR.E
1255   527.8199 1053.6252 1053.6182 6.67 1 47 5.8e-005 +1Score > 17 indicates identity U K.LSLELVPQR.L
1314   361.1786 1080.5140 1080.5101 3.58 0 28 0.011 +1Score > 21 indicates identity U R.DVHPTHYGR.V
1390   560.8047 1119.5948 1119.6036 -7.85 1 3 2.6 +2Score > 21 indicates identity
Score > 20 indicates homology		 U E.KAQQYIVDR.R
1478   578.3442 1154.6738 1154.6659 6.88 1 51 2.3e-005 +1Score > 17 indicates identity U K.QLIDELVAVR.H
1485 +1 578.8210 1155.6274 1155.6499 -19.4 1 50 6.1e-005 +1Score > 21 indicates identity U K.QLIDELVAVR.H + Deamidated (NQ)
1539   392.8537 1175.5393 1175.5360 2.79 0 49 7.5e-005 +1Score > 20 indicates identity U R.SPGVFFDHDR.G
1563   593.8217 1185.6288 1185.6064 19.0 1 0 8.1 +5Score > 22 indicates identity U K.AGVKQLDVPME.Y
1667   617.8517 1233.6888 1233.6718 13.8 0 51 2.5e-005 +1Score > 17 indicates identity U K.GTVIDVQVFTR.D
1828   441.8957 1322.6653 1322.6579 5.59 0 55 1.9e-005 +1Score > 20 indicates identity U K.GIVDDIDHLGNR.R
1873   677.3732 1352.7318 1352.7300 1.38 0 61 3.4e-006 +1Score > 19 indicates identity U K.LQQGDDLAPGVLK.I
1943   693.3758 1384.7370 1384.7310 4.33 0 61 5.3e-006 +1Score > 20 indicates identity
Score > 20 indicates homology		 U R.SALNGQVVDGGAGLK.K
2116   737.8554 1473.6962 1473.6888 5.03 1 63 2.1e-006 +1Score > 21 indicates identity
Score > 19 indicates homology		 U R.TNQYGFLESPYR.V
2455   811.9255 1621.8364 1621.8312 3.26 0 94 1.9e-009 +1Score > 20 indicates identity U R.STGSYSLVTQQPLGGK.A
2619   565.6517 1693.9333 1693.9185 8.72 1 27 0.0062 +1Score > 17 indicates identity U R.MNVGQILETHLGLAAK.G
2736   874.4435 1746.8724 1746.8611 6.51 1 90 8.8e-009 +1Score > 22 indicates identity U K.LADLPESGQMVLFDGR.T
3506   728.0535 2181.1387 2181.1066 14.7 1 87 8.4e-009 +1Score > 19 indicates identity U K.QVVSVAASLIPFLEHDDANR.A + Deamidated (NQ)
3552   1103.5690 2205.1234 2205.1106 5.83 1 96 1.4e-009 +1Score > 19 indicates identity U K.SVFPIISYSGNAALEYVGYR.L
3554   736.3828 2206.1266 2206.0946 14.5 1 36 0.0013 +1Score > 20 indicates identity U K.SVFPIISYSGNAALEYVGYR.L + Deamidated (NQ)
3573   741.3990 2221.1752 2221.1340 18.5 0 42 0.00018 +1Score > 17 indicates identity U K.LPDVMDVPYLLAIQLDSYR.E + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q88EK8_PSEPK 21 31236 9.1
Opine ABC transporter, permease protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4454 PE=3 SV=1

+10

Accession Score Description
1 Q88FA8_PSEPK 775 Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1
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