MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 313)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 32 Next 

+81

Accession Score Description
1 FTSZ_PSEPK 185 Cell division protein FtsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

+82

Accession Score Description
1 AMPA_PSEPK 178 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1

+83

Accession Score Description
1 Q88CY3_PSEPK 172 Glutamine synthetase OS=Pseudomonas putida (strain KT2440) GN=glnA PE=3 SV=1

+84

Accession Score Description
1 RL21_PSEPK 171 50S ribosomal protein L21 OS=Pseudomonas putida (strain KT2440) GN=rplU PE=3 SV=2

+85

Accession Score Description
1 Q88QZ6_PSEPK 171 Acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=aceF PE=3 SV=1

+86

Accession Score Description
1 Q88QD0_PSEPK 164 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0564 PE=4 SV=1

-87

Accession Score Description
1 Q88PL6_PSEPK 162 Preprotein translocase, YajC subunit OS=Pseudomonas putida (strain KT2440) GN=yajC PE=4 SV=1
Score Mass Matches Sequences emPAI
87.1 Q88PL6_PSEPK 162 11894 8 (5) 3 (3) 1.51
Preprotein translocase, YajC subunit OS=Pseudomonas putida (strain KT2440) GN=yajC PE=4 SV=1

-8 peptide matches (6 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
760   841.5166 840.5093 840.5069 2.91 0 61 1.8e-006 +1Score > 16 indicates identity U K.GAIAATLPK.G
761 +1 421.2623 840.5100 840.5069 3.77 0 42 0.00013 +1Score > 16 indicates identity U K.GAIAATLPK.G
981   929.5444 928.5371 928.5341 3.20 0 34 0.0015 +1Score > 21 indicates identity
Score > 18 indicates homology		 U K.NLLSNLQK.G
983 +1 465.2774 928.5402 928.5341 6.57 0 40 0.00055 +1Score > 20 indicates identity U K.NLLSNLQK.G
1622   608.8138 1215.6130 1215.6095 2.88 1 58 1.3e-005 +1Score > 22 indicates identity
Score > 21 indicates homology		 U K.GDEVVTNGGIAGK.I
1623   609.3114 1216.6082 1216.5936 12.1 1 2 6 +6Score > 22 indicates identity U K.GDEVVTNGGIAGK.I + Deamidated (NQ)

+88

Accession Score Description
1 Q88FB3_PSEPK 161 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas putida (strain KT2440) GN=sucD PE=3 SV=1

+89

Accession Score Description
1 RL6_PSEPK 156 50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1

+90

Accession Score Description
1 Q88FM4_PSEPK 156 3-methylcrotonyl-CoA carboxylase, beta subunit, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4065 PE=4 SV=1
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