MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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81| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FTSZ_PSEPK | 185 | Cell division protein FtsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 |
82| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPK | 178 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1 |
83| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CY3_PSEPK | 172 | Glutamine synthetase OS=Pseudomonas putida (strain KT2440) GN=glnA PE=3 SV=1 |
84| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEPK | 171 | 50S ribosomal protein L21 OS=Pseudomonas putida (strain KT2440) GN=rplU PE=3 SV=2 |
85| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QZ6_PSEPK | 171 | Acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=aceF PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 85.1 | Q88QZ6_PSEPK |
171 | 55791 | 13 (5) | 10 (5) | 0.22 | |
| Acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=aceF PE=3 SV=1 | |||||||
13 peptide matches (10 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 212 |
487.3255 | 486.3182 | 486.3166 | 3.39 | 0 | 20 | 0.17 | 3Score > 24 indicates identity |
K.AGVIK.E | ||
| 283 |
3 |
586.3860 | 585.3787 | 585.3737 | 8.50 | 0 | 6 | 1.4 | 6Score > 20 indicates identity |
U | E.LLVLE.A |
| 353 |
318.1801 | 634.3456 | 634.3438 | 2.85 | 1 | 16 | 0.14 | 2Score > 21 indicates identity |
E.LEAFR.V | ||
| 702 |
407.2778 | 812.5410 | 812.5371 | 4.83 | 1 | 43 | 5.5e-005 | 1Score > 13 indicates identity |
U | K.IIEVLVK.V | |
| 1171 |
505.3573 | 1008.7000 | 1008.6947 | 5.31 | 0 | 41 | 7.3e-005 | 1Score > 13 indicates identity |
U | K.LTVLPLLLK.A | |
| 1431 |
567.2758 | 1132.5370 | 1132.5223 | 13.0 | 0 | 1 | 5.1 | 3Score > 21 indicates identity |
U | K.ATMQPVWDGK.A + Deamidated (NQ) | |
| 2095 |
731.3697 | 1460.7248 | 1460.7147 | 6.94 | 1 | 55 | 2.4e-005 | 1Score > 21 indicates identity |
U | K.ELPDFNSSLAPSGK.A | |
| 2499 |
820.9615 | 1639.9084 | 1639.9045 | 2.38 | 0 | 72 | 1.7e-007 | 1Score > 17 indicates identity |
U | K.VAGAAPAAAPAPAAAPAPAK.A | |
| 3457 |
718.4102 | 2152.2088 | 2152.1933 | 7.21 | 0 | 22 | 0.008 | 1Score > 14 indicates identity |
U | K.YVHIGFAVDTPDGLLVPVIK.N | |
| 4036 |
880.4657 | 2638.3753 | 2638.3350 | 15.3 | 1 | 8 | 0.45 | 1Score > 17 indicates identity |
U | K.AEAAPAAAPAAAAPAAAPAPAATAPAAGSNAK.V + Deamidated (NQ) |
86| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QD0_PSEPK | 164 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0564 PE=4 SV=1 |
87| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PL6_PSEPK | 162 | Preprotein translocase, YajC subunit OS=Pseudomonas putida (strain KT2440) GN=yajC PE=4 SV=1 |
88| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FB3_PSEPK | 161 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas putida (strain KT2440) GN=sucD PE=3 SV=1 |
89| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL6_PSEPK | 156 | 50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1 |
90| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FM4_PSEPK | 156 | 3-methylcrotonyl-CoA carboxylase, beta subunit, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4065 PE=4 SV=1 |
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