MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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81| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FTSZ_PSEPK | 185 | Cell division protein FtsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 |
82| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_PSEPK | 178 | Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1 |
83| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88CY3_PSEPK | 172 | Glutamine synthetase OS=Pseudomonas putida (strain KT2440) GN=glnA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 83.1 | Q88CY3_PSEPK |
172 | 51936 | 10 (6) | 9 (6) | 0.30 | |
| Glutamine synthetase OS=Pseudomonas putida (strain KT2440) GN=glnA PE=3 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 443 |
344.2242 | 686.4338 | 686.4327 | 1.73 | 0 | 20 | 0.03 | 1Score > 18 indicates identity |
U | K.SVQLIK.D | |
| 474 |
351.7013 | 701.3880 | 701.3860 | 2.85 | 0 | 16 | 0.19 | 1Score > 21 indicates identity |
U | K.WIDLR.F | |
| 511 |
361.2166 | 720.4186 | 720.4170 | 2.26 | 0 | 9 | 0.33 | 1Score > 17 indicates identity |
U | K.FNTLVK.K | |
| 513 |
361.7017 | 721.3888 | 721.4010 | -16.9 | 0 | 1 | 3 | 7Score > 21 indicates identity Score > 18 indicates homology |
U | K.FNTLVK.K + Deamidated (NQ) | |
| 829 |
430.7487 | 859.4828 | 859.4803 | 2.91 | 0 | 35 | 0.0021 | 1Score > 21 indicates identity |
U | R.IPYVGSPK.A | |
| 1889 |
455.2305 | 1362.6697 | 1362.6680 | 1.19 | 0 | 43 | 0.00049 | 1Score > 22 indicates identity |
U | K.YVVHNVADAYGR.T | |
| 2222 |
757.3701 | 1512.7256 | 1512.7209 | 3.17 | 0 | 70 | 4.8e-007 | 1Score > 20 indicates identity |
U | K.ALNGFTNPSTNSYK.R | |
| 2792 |
591.6389 | 1771.8949 | 1771.8669 | 15.8 | 1 | 19 | 0.079 | 1Score > 20 indicates identity |
U | K.GGVFSDDFIDAFIELK.S | |
| 3917 |
832.4284 | 2494.2634 | 2494.2268 | 14.7 | 1 | 58 | 5.5e-006 | 1Score > 18 indicates identity |
U | K.NTFSGEGYAGLSDTALYFIGGIIK.H + Deamidated (NQ) | |
| 4118 |
912.4805 | 2734.4197 | 2734.3676 | 19.0 | 0 | 43 | 0.00016 | 1Score > 18 indicates identity |
U | R.FPDPSANPYLAFAALLMAGLDGIQNK.I + Deamidated (NQ) |
84| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEPK | 171 | 50S ribosomal protein L21 OS=Pseudomonas putida (strain KT2440) GN=rplU PE=3 SV=2 |
85| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QZ6_PSEPK | 171 | Acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=aceF PE=3 SV=1 |
86| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QD0_PSEPK | 164 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0564 PE=4 SV=1 |
87| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PL6_PSEPK | 162 | Preprotein translocase, YajC subunit OS=Pseudomonas putida (strain KT2440) GN=yajC PE=4 SV=1 |
88| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FB3_PSEPK | 161 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas putida (strain KT2440) GN=sucD PE=3 SV=1 |
89| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL6_PSEPK | 156 | 50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1 |
90| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FM4_PSEPK | 156 | 3-methylcrotonyl-CoA carboxylase, beta subunit, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4065 PE=4 SV=1 |
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