MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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71| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P92_PSEPK | 201 | Toluene tolerance ABC transporter, periplasmic substrate-binding protein OS=Pseudomonas putida (strain KT2440) GN=ttg2C PE=4 SV=1 |
72| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KI7_PSEPK | 201 | DNA-binding protein HU-beta OS=Pseudomonas putida (strain KT2440) GN=hupB PE=3 SV=1 |
73| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NH1_PSEPK | 199 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1238 PE=4 SV=1 |
74| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DAVT_PSEPK | 197 | 5-aminovalerate aminotransferase DavT OS=Pseudomonas putida (strain KT2440) GN=davT PE=1 SV=1 |
75| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NM8_PSEPK | 193 | Ribonucleoside-diphosphate reductase OS=Pseudomonas putida (strain KT2440) GN=nrdA PE=3 SV=1 |
76| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88M60_PSEPK | 192 | Peptidyl-prolyl cis-trans isomerase OS=Pseudomonas putida (strain KT2440) GN=fklB-2 PE=4 SV=1 |
77| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N72_PSEPK | 191 | Cell division protein ftsA OS=Pseudomonas putida (strain KT2440) GN=ftsA PE=3 SV=1 |
78| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEPK | 189 | Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1 |
79| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88K44_PSEPK | 186 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2448 PE=4 SV=1 |
80| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KI6_PSEPK | 186 | Peptidylprolyl isomerase OS=Pseudomonas putida (strain KT2440) GN=PP_2304 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 80.1 | Q88KI6_PSEPK |
186 | 68538 | 12 (7) | 11 (6) | 0.26 | |
| Peptidylprolyl isomerase OS=Pseudomonas putida (strain KT2440) GN=PP_2304 PE=4 SV=1 | |||||||
12 peptide matches (12 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 343 |
314.7116 | 627.4086 | 627.4068 | 2.97 | 0 | 9 | 0.7 | 6Score > 20 indicates identity |
U | K.LIGGLR.D | |
| 350 |
632.3749 | 631.3676 | 631.3653 | 3.66 | 1 | 14 | 0.24 | 3Score > 20 indicates identity |
U | K.DKLTR.D | |
| 417 |
673.4011 | 672.3938 | 672.3918 | 2.94 | 1 | 12 | 0.75 | 6Score > 23 indicates identity |
U | E.KQQIR.R + Deamidated (NQ) | |
| 419 |
337.2169 | 672.4192 | 672.4282 | -13.4 | 1 | 4 | 1.4 | 7Score > 18 indicates identity |
U | K.GLISRK.L | |
| 1293 |
536.3285 | 1070.6424 | 1070.6336 | 8.30 | 0 | 13 | 0.13 | 1Score > 17 indicates identity |
U | R.DGSLVVLQLK.G | |
| 1518 |
585.3210 | 1168.6274 | 1168.6200 | 6.34 | 0 | 52 | 2.4e-005 | 1Score > 19 indicates identity |
U | K.LQDGQVSAPVR.T | |
| 1659 |
411.5733 | 1231.6981 | 1231.6925 | 4.54 | 0 | 28 | 0.0061 | 1Score > 19 indicates identity |
U | K.DKPVYGSVVLR.D | |
| 1796 |
436.9241 | 1307.7505 | 1307.7449 | 4.28 | 1 | 23 | 0.0094 | 1Score > 15 indicates identity |
U | R.KPEQLPLDAVAK.N | |
| 2533 |
828.4783 | 1654.9420 | 1654.9294 | 7.64 | 1 | 31 | 0.0016 | 1Score > 15 indicates identity |
U | K.LLGVQAPEVPSFASLK.D | |
| 3103 |
985.9932 | 1969.9718 | 1969.9382 | 17.1 | 0 | 104 | 2.5e-010 | 1Score > 21 indicates identity |
U | R.TGLAGSSFVTDQQVDAFAR.L + Deamidated (NQ) | |
| 3104 |
657.6657 | 1969.9753 | 1969.9382 | 18.8 | 0 | 18 | 0.05 | 1Score > 21 indicates identity Score > 18 indicates homology |
U | R.TGLAGSSFVTDQQVDAFAR.L + Deamidated (NQ) | |
| 3834 |
801.7399 | 2402.1979 | 2402.1601 | 15.7 | 1 | 37 | 0.00087 | 1Score > 19 indicates identity |
U | K.QLQDAAYEASDLAQPAQDLNLK.V + Deamidated (NQ) |
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