MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 313)

Page: 1 2 3 4 5 6 … 32 Next 

+1

Accession Score Description
1 Q88NM2_PSEPK 4307 Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 2609 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
2 EFTU1_PSEPK 2412 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1

+3

Accession Score Description
1 ATPB_PSEPK 2344 ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1

+4

Accession Score Description
1 Q88ES5_PSEPK 2322 Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1

+5

Accession Score Description
1 Q88L46_PSEPK 1745 Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1

+6

Accession Score Description
1 ATPA_PSEPK 1711 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1

+7

Accession Score Description
1 Q88FB0_PSEPK 1295 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1

-8

Accession Score Description
1 Q88FA7_PSEPK 1233 Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1
Score Mass Matches Sequences emPAI
8.1 Q88FA7_PSEPK 1233 63979 42 (35) 21 (18) 1.97
Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1

-42 peptide matches (36 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
250   544.3481 543.3408 543.3380 5.15 0 30 0.0098 +1Score > 23 indicates identity U R.IANVK.I
261   559.3227 558.3154 558.3126 5.13 0 26 0.07 +1Score > 26 indicates identity R.DVVAR.S
325   309.6958 617.3770 617.3748 3.59 0 29 0.0021 +1Score > 14 indicates identity U K.TAVVTK.V
326   618.3850 617.3777 617.3748 4.68 0 51 1.3e-005 +1Score > 15 indicates identity U K.TAVVTK.V
519   362.7220 723.4294 723.4279 2.12 0 16 0.025 +1Score > 13 indicates identity U K.DHVLLK.L
540   732.4055 731.3982 731.3966 2.19 0 34 0.0022 +1Score > 20 indicates identity U R.GVNFAPK.T
541   366.7067 731.3988 731.3966 3.04 0 26 0.0064 +1Score > 20 indicates identity
Score > 17 indicates homology		 U R.GVNFAPK.T
759   419.7445 837.4744 837.4708 4.33 1 42 0.00016 +1Score > 16 indicates identity U R.AAGLHLEK.A
880   888.4854 887.4781 887.4753 3.22 0 40 0.00029 +1Score > 21 indicates identity
Score > 17 indicates homology		 U K.TVPAFEPK.V
881   444.7464 887.4782 887.4753 3.36 0 19 0.058 +1Score > 21 indicates identity
Score > 19 indicates homology		 U K.TVPAFEPK.V
882   444.7578 887.5010 887.4977 3.74 1 17 0.12 +1Score > 20 indicates identity U K.RGVNFAPK.T
889   894.4421 893.4348 893.4355 -0.77 0 22 0.039 +1Score > 21 indicates identity U K.SQAFNTAR.I
890   447.7255 893.4364 893.4355 1.04 0 45 0.00023 +1Score > 21 indicates identity U K.SQAFNTAR.I
1059   956.5555 955.5482 955.5451 3.31 1 62 2.7e-006 +1Score > 19 indicates identity U K.GIEQLAGLR.D
1061 +2 478.7827 955.5508 955.5451 6.06 1 53 2.4e-005 +1Score > 19 indicates identity U K.GIEQLAGLR.D
1100   487.2756 972.5366 972.5352 1.44 0 38 0.0012 +1Score > 22 indicates identity
Score > 22 indicates homology		 U K.ATVLATGGAGR.I
1101   973.5499 972.5426 972.5352 7.58 0 50 7.8e-005 +1Score > 22 indicates identity U K.ATVLATGGAGR.I
1169   503.7581 1005.5016 1005.4978 3.78 1 68 1.1e-006 +1Score > 21 indicates identity U R.TSGEDVASLK.R
1336   547.3093 1092.6040 1092.6040 0.069 0 58 5.3e-006 +1Score > 17 indicates identity U R.AALQLAQGGHK.T
1338 +1 365.2096 1092.6070 1092.6040 2.74 0 18 0.043 +1Score > 17 indicates identity U R.AALQLAQGGHK.T
1517   584.8218 1167.6290 1167.6248 3.66 1 77 9.6e-008 +1Score > 19 indicates identity U K.EIIAGNGVGPNK.D
1749   427.5641 1279.6705 1279.6673 2.47 0 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 U R.IYQRPFGGQSK.D
1750   640.8430 1279.6714 1279.6673 3.23 0 57 1.2e-005 +1Score > 20 indicates identity U R.IYQRPFGGQSK.D
1964   695.8277 1389.6408 1389.6372 2.62 0 71 4.4e-007 +1Score > 20 indicates identity U R.DASDTDVDVALNR.L
1967   696.3300 1390.6454 1390.6212 17.4 0 52 3.9e-005 +1Score > 20 indicates identity U R.DASDTDVDVALNR.L + Deamidated (NQ)
2089   487.2839 1458.8299 1458.8195 7.13 0 74 1e-007 +1Score > 17 indicates identity U R.LGGNSLLDLVVFGR.A
2090 +2 730.4236 1458.8326 1458.8195 9.03 0 114 9.5e-012 +1Score > 17 indicates identity U R.LGGNSLLDLVVFGR.A
2547   833.4595 1664.9044 1664.8998 2.78 0 85 1.3e-008 +1Score > 19 indicates identity U R.TGHALLHTLYQGNLK.A
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 19 indicates identity
2548   417.2343 1664.9081 1664.8998 4.97 0 36 0.00096 -1Score > 19 indicates identity U R.TGHALLHTLYQGNLK.A
No other peptide matches in query
2549   417.4842 1665.9077 1665.8838 14.3 0 25 0.011 +1Score > 18 indicates identity U R.TGHALLHTLYQGNLK.A + Deamidated (NQ)
2550   556.3102 1665.9088 1665.8838 15.0 0 24 0.016 +1Score > 18 indicates identity U R.TGHALLHTLYQGNLK.A + Deamidated (NQ)
2849   601.9822 1802.9248 1802.9091 8.72 1 24 0.021 +1Score > 20 indicates identity U K.AGTTFLNEYYAVDLVK.N
2850 +1 902.4697 1802.9248 1802.9091 8.76 1 58 9.1e-006 +1Score > 20 indicates identity U K.AGTTFLNEYYAVDLVK.N
2853   902.9692 1803.9238 1803.8931 17.1 1 60 6.4e-006 +1Score > 21 indicates identity U K.AGTTFLNEYYAVDLVK.N + Deamidated (NQ)
3156   1002.5248 2003.0350 2003.0510 -7.96 0 8 1 +1Score > 20 indicates identity
Score > 20 indicates homology		 U M.ASIPTISFDAIIIGGGGAGMR.A
3454   1076.5631 2151.1116 2151.0994 5.70 0 141 3.6e-014 +1Score > 19 indicates identity U R.IYASTTNALINTGDGIGMALR.A

+9

Accession Score Description
1 RPOB_PSEPK 843 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

+10

Accession Score Description
1 Q88FA8_PSEPK 775 Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1
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