MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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71| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88P92_PSEPK | 201 | Toluene tolerance ABC transporter, periplasmic substrate-binding protein OS=Pseudomonas putida (strain KT2440) GN=ttg2C PE=4 SV=1 |
72| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KI7_PSEPK | 201 | DNA-binding protein HU-beta OS=Pseudomonas putida (strain KT2440) GN=hupB PE=3 SV=1 |
73| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NH1_PSEPK | 199 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1238 PE=4 SV=1 |
74| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DAVT_PSEPK | 197 | 5-aminovalerate aminotransferase DavT OS=Pseudomonas putida (strain KT2440) GN=davT PE=1 SV=1 |
75| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NM8_PSEPK | 193 | Ribonucleoside-diphosphate reductase OS=Pseudomonas putida (strain KT2440) GN=nrdA PE=3 SV=1 |
76| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88M60_PSEPK | 192 | Peptidyl-prolyl cis-trans isomerase OS=Pseudomonas putida (strain KT2440) GN=fklB-2 PE=4 SV=1 |
77| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88N72_PSEPK | 191 | Cell division protein ftsA OS=Pseudomonas putida (strain KT2440) GN=ftsA PE=3 SV=1 |
78| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEPK | 189 | Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1 |
79| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88K44_PSEPK | 186 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2448 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 79.1 | Q88K44_PSEPK |
186 | 46959 | 8 (5) | 7 (4) | 0.27 | |
| Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2448 PE=4 SV=1 | |||||||
8 peptide matches (8 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 403 |
336.1896 | 670.3646 | 670.3762 | -17.3 | 0 | 5 | 1.3 | 8Score > 18 indicates identity |
U | E.QTPAVR.I | |
| 480 |
703.4034 | 702.3961 | 702.4064 | -14.7 | 0 | 18 | 0.15 | 5Score > 22 indicates identity |
U | K.AFPQLK.N | |
| 610 |
386.2402 | 770.4658 | 770.4650 | 1.09 | 1 | 37 | 0.0019 | 1Score > 22 indicates identity |
U | K.VLAEALR.G | |
| 1599 |
402.2202 | 1203.6388 | 1203.6360 | 2.28 | 0 | 3 | 1 | 2Score > 20 indicates identity Score > 15 indicates homology |
U | R.GANPVVHTPQGK.V | |
| 1604 |
604.8496 | 1207.6846 | 1207.6714 | 11.0 | 0 | 62 | 1.1e-006 | 1Score > 15 indicates identity |
U | R.LIFGGGVVYGAR.D | |
| 1847 |
446.2599 | 1335.7579 | 1335.7510 | 5.14 | 1 | 54 | 6e-006 | 1Score > 14 indicates identity |
U | R.DPANIEAIIRPK.M | |
| 3282 |
692.0687 | 2073.1843 | 2073.1510 | 16.1 | 1 | 17 | 0.022 | 1Score > 13 indicates identity |
U | K.FIIVAGNAYLGNLVPELAAK.S + Deamidated (NQ) | |
| 3283 |
1037.6008 | 2073.1870 | 2073.1510 | 17.4 | 1 | 79 | 1.4e-008 | 1Score > 13 indicates identity |
U | K.FIIVAGNAYLGNLVPELAAK.S + Deamidated (NQ) |
80| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KI6_PSEPK | 186 | Peptidylprolyl isomerase OS=Pseudomonas putida (strain KT2440) GN=PP_2304 PE=4 SV=1 |
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