MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 313)


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+71

Accession Score Description
1 Q88P92_PSEPK 201 Toluene tolerance ABC transporter, periplasmic substrate-binding protein OS=Pseudomonas putida (strain KT2440) GN=ttg2C PE=4 SV=1

+72

Accession Score Description
1 Q88KI7_PSEPK 201 DNA-binding protein HU-beta OS=Pseudomonas putida (strain KT2440) GN=hupB PE=3 SV=1

+73

Accession Score Description
1 Q88NH1_PSEPK 199 Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1238 PE=4 SV=1

+74

Accession Score Description
1 DAVT_PSEPK 197 5-aminovalerate aminotransferase DavT OS=Pseudomonas putida (strain KT2440) GN=davT PE=1 SV=1

+75

Accession Score Description
1 Q88NM8_PSEPK 193 Ribonucleoside-diphosphate reductase OS=Pseudomonas putida (strain KT2440) GN=nrdA PE=3 SV=1

+76

Accession Score Description
1 Q88M60_PSEPK 192 Peptidyl-prolyl cis-trans isomerase OS=Pseudomonas putida (strain KT2440) GN=fklB-2 PE=4 SV=1

+77

Accession Score Description
1 Q88N72_PSEPK 191 Cell division protein ftsA OS=Pseudomonas putida (strain KT2440) GN=ftsA PE=3 SV=1

-78

Accession Score Description
1 IF2_PSEPK 189 Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1
Score Mass Matches Sequences emPAI
78.1 IF2_PSEPK 189 91576 11 (5) 11 (5) 0.13
Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1

-11 peptide matches (11 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
202   474.2695 473.2622 473.2710 -18.6 1 4 1.4 +3Score > 29 indicates identity
Score > 18 indicates homology
R.GRGGK.S
423   338.2064 674.3982 674.3963 2.89 0 15 0.091 +1Score > 17 indicates identity U M.TQVTVK.E
445   688.3691 687.3618 687.3664 -6.60 1 0 11 +7Score > 23 indicates identity U R.ADAGARK.I
543   367.7147 733.4148 733.4123 3.53 1 5 1.5 +6Score > 19 indicates identity U R.EVALFR.Q
558   372.2193 742.4240 742.4225 2.11 0 3 3.1 +8Score > 22 indicates identity
Score > 20 indicates homology
U E.AVLLQAE.I
682   400.7674 799.5202 799.5167 4.41 0 20 0.011 +1Score > 13 indicates identity U K.TLNIVLK.T
984   465.3068 928.5990 928.5957 3.62 1 4 0.42 +2Score > 13 indicates identity U K.KTLNIVLK.T + Deamidated (NQ)
1114   490.7776 979.5406 979.5338 6.96 0 39 0.00037 +1Score > 17 indicates identity U K.TSLLDYIR.R
2060   720.4216 1438.8286 1438.8144 9.91 0 73 1.4e-007 +1Score > 17 indicates identity U R.GPVATILVQDGTLR.Q
2317   522.2715 1563.7927 1563.7906 1.30 0 23 0.024 +1Score > 20 indicates identity
Score > 19 indicates homology
U R.NQHGFQNPTGPVIR.D
4272   973.8608 2918.5606 2918.5138 16.0 1 98 2.5e-010 +1Score > 14 indicates identity U R.VIGGGVGGITESDANLALASNAVLFGFNVR.A + Deamidated (NQ)

+79

Accession Score Description
1 Q88K44_PSEPK 186 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2448 PE=4 SV=1

+80

Accession Score Description
1 Q88KI6_PSEPK 186 Peptidylprolyl isomerase OS=Pseudomonas putida (strain KT2440) GN=PP_2304 PE=4 SV=1
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