MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 313)

Page: Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 32 Next 

+61

Accession Score Description
1 Q88DD5_PSEPK 231 HflK protein OS=Pseudomonas putida (strain KT2440) GN=hflK PE=4 SV=1

+62

Accession Score Description
Family member distances as a dendrogram 1 Q88H57_PSEPK 217 Cobalamin biosynthesis protein CobW OS=Pseudomonas putida (strain KT2440) GN=cobW PE=4 SV=1
3 Q88JM1_PSEPK 47 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2628 PE=3 SV=1
4 Q88FE7_PSEPK 30 description
2 Q88IR3_PSEPK 94 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2936 PE=4 SV=1

+63

Accession Score Description
1 RISB_PSEPK 216 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain KT2440) GN=ribH PE=3 SV=1

+64

Accession Score Description
1 Q88MT1_PSEPK 213 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1487 PE=4 SV=1

+65

Accession Score Description
1 Q88FS1_PSEPK 212 Isocitrate dehydrogenase, NADP-dependent, monomeric-type OS=Pseudomonas putida (strain KT2440) GN=PP_4012 PE=4 SV=1

+66

Accession Score Description
1 Q88KG8_PSEPK 209 Outer membrane lipoprotein OprI OS=Pseudomonas putida (strain KT2440) GN=oprI PE=4 SV=1

+67

Accession Score Description
1 Q88PE1_PSEPK 208 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1

+68

Accession Score Description
1 YIDC_PSEPK 208 Membrane protein insertase YidC OS=Pseudomonas putida (strain KT2440) GN=yidC PE=3 SV=1

+69

Accession Score Description
1 RL28_PSEPK 206 50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1

-70

Accession Score Description
1 RS7_PSEPK 202 30S ribosomal protein S7 OS=Pseudomonas putida (strain KT2440) GN=rpsG PE=3 SV=1
Score Mass Matches Sequences emPAI
70.1 RS7_PSEPK 202 17567 11 (11) 6 (6) 1.42
30S ribosomal protein S7 OS=Pseudomonas putida (strain KT2440) GN=rpsG PE=3 SV=1

-11 peptide matches (7 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
736   415.2195 828.4244 828.4229 1.89 1 35 0.0017 +1Score > 20 indicates identity U R.EILDDPK.Y
858   879.4956 878.4883 878.4861 2.48 0 27 0.0058 +1Score > 17 indicates identity U K.YGSQILAK.F
961   461.7454 921.4762 921.4708 5.87 0 24 0.029 +1Score > 21 indicates identity U R.WLVDYAR.K
1311 +1 539.8158 1077.6170 1077.6070 9.32 0 46 4.2e-005 +1Score > 15 indicates identity U R.IVYGALDTVK.A
1681 +2 619.8787 1237.7428 1237.7282 11.9 1 42 5.7e-005 +1Score > 13 indicates identity U K.ALDAIAPLVEVK.S
2666 +1 572.3148 1713.9226 1713.9162 3.70 1 41 0.00034 +1Score > 19 indicates identity U R.VGGATYQVPVEVRPSR.R
2667   857.9695 1713.9244 1713.9162 4.80 1 44 0.00019 +1Score > 19 indicates identity U R.VGGATYQVPVEVRPSR.R
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