MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 313)

Page: 1 2 3 4 5 6 … 32 Next 

+1

Accession Score Description
1 Q88NM2_PSEPK 4307 Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 2609 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
2 EFTU1_PSEPK 2412 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1

+3

Accession Score Description
1 ATPB_PSEPK 2344 ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1

+4

Accession Score Description
1 Q88ES5_PSEPK 2322 Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1

+5

Accession Score Description
1 Q88L46_PSEPK 1745 Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1

+6

Accession Score Description
1 ATPA_PSEPK 1711 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1

-7

Accession Score Description
1 Q88FB0_PSEPK 1295 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1
Score Mass Matches Sequences emPAI
7.1 Q88FB0_PSEPK 1295 42408 48 (39) 15 (12) 1.58
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1

-48 peptide matches (24 non-duplicate, 24 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
252   545.3285 544.3212 544.3221 -1.52 0 20 0.079 +1Score > 22 indicates identity R.LIDGK.E
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 22 indicates identity
269   573.3618 572.3545 572.3533 2.08 1 34 0.0041 -1Score > 22 indicates identity U M.AIEIK.A
2.08 1 34 0.0041 1 ALELK  
2.08 0 30 0.0087 3 ALQIK   + Deamidated (NQ)
2.08 1 20 0.095 4 IAELK  
-17.6 1 18 0.16 5 AGKGLK  
2.08 0 18 0.17 6 IAQIK   + Deamidated (NQ)
2.08 0 18 0.17 6 IAQLK   + Deamidated (NQ)
2.08 0 18 0.17 6 LAQIK   + Deamidated (NQ)
2.08 1 16 0.24 9 AILEK  
2.08 1 16 0.24 9 ALLEK  
283 +4 586.3860 585.3787 585.3737 8.50 0 25 0.02 +1Score > 20 indicates identity U R.LLLDI.-
375   326.1716 650.3286 650.3275 1.72 1 21 0.064 +1Score > 21 indicates identity U K.DLFEK.T
376   651.3369 650.3296 650.3275 3.22 1 25 0.023 +1Score > 21 indicates identity U K.DLFEK.T
478   352.2034 702.3922 702.3912 1.53 1 18 0.14 +1Score > 22 indicates identity U K.AATEALK.R
480   703.4034 702.3961 702.3912 7.05 1 41 0.0007 +1Score > 22 indicates identity U K.AATEALK.R
585   380.2203 758.4260 758.4221 5.18 1 12 0.81 +2Score > 24 indicates identity U K.RVPMTR.L
798 +3 426.7901 851.5656 851.5593 7.48 0 41 7.9e-005 +1Score > 13 indicates identity U R.GLVVPVLR.N
799 +1 852.5732 851.5659 851.5593 7.80 0 41 8.1e-005 +1Score > 13 indicates identity U R.GLVVPVLR.N
916   901.5017 900.4944 900.4916 3.09 1 62 7.5e-006 +1Score > 23 indicates identity U K.EDVVAAVAK.K
917   451.2555 900.4964 900.4916 5.34 1 53 5.4e-005 +1Score > 23 indicates identity U K.EDVVAAVAK.K
1024   943.4783 942.4710 942.4770 -6.38 1 19 0.053 +1Score > 19 indicates identity U K.NLLEDPSR.L
1025   472.2466 942.4786 942.4770 1.71 1 25 0.015 +1Score > 19 indicates identity U K.NLLEDPSR.L
1029   945.4904 944.4831 944.4702 13.7 1 12 0.7 +3Score > 23 indicates identity U R.DELIVDIE.T
1300 +3 538.2888 1074.5630 1074.5572 5.41 0 59 9.4e-006 +1Score > 21 indicates identity U R.LGFMSFFVK.A
1304 +1 1075.5811 1074.5738 1074.5572 15.4 0 49 7.4e-005 +1Score > 20 indicates identity U R.LGFMSFFVK.A
1397 +3 560.8389 1119.6632 1119.6540 8.29 1 68 1.6e-007 +1Score > 13 indicates identity U K.EAVTFLVTIK.N
3032   957.4798 1912.9450 1912.9319 6.85 1 101 4.1e-010 +1Score > 20 indicates identity U K.APTFPESVADGTVATWHK.K
4326 +1 998.4952 2992.4638 2992.4203 14.5 0 88 5.1e-009 +1Score > 17 indicates identity U R.FPAVNASIDGNDIVYHGFADVGVAVSSDR.G + Deamidated (NQ)
4327 +7 998.4958 2992.4656 2992.4203 15.1 0 79 3.8e-008 +1Score > 17 indicates identity U R.FPAVNASIDGNDIVYHGFADVGVAVSSDR.G + Deamidated (NQ)
4334   998.8274 2993.4604 2993.4043 18.7 0 57 6.3e-006 +1Score > 17 indicates identity U R.FPAVNASIDGNDIVYHGFADVGVAVSSDR.G + 2 Deamidated (NQ)
4433   788.1466 3148.5573 3148.5214 11.4 1 29 0.0038 +1Score > 17 indicates identity U K.RFPAVNASIDGNDIVYHGFADVGVAVSSDR.G + Deamidated (NQ)
4438 +1 788.3969 3149.5585 3149.5054 16.9 1 41 0.00033 +1Score > 19 indicates identity U K.RFPAVNASIDGNDIVYHGFADVGVAVSSDR.G + 2 Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
SYE_PSEPK 34 56789 7.1
Glutamate--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=gltX PE=3 SV=1

+8

Accession Score Description
1 Q88FA7_PSEPK 1233 Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1

+9

Accession Score Description
1 RPOB_PSEPK 843 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

+10

Accession Score Description
1 Q88FA8_PSEPK 775 Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1
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