MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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61| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DD5_PSEPK | 231 | HflK protein OS=Pseudomonas putida (strain KT2440) GN=hflK PE=4 SV=1 |
62| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88H57_PSEPK | 217 | Cobalamin biosynthesis protein CobW OS=Pseudomonas putida (strain KT2440) GN=cobW PE=4 SV=1 | |
| 3 | Q88JM1_PSEPK | 47 | ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2628 PE=3 SV=1 | |
| 4 | Q88FE7_PSEPK | 30 | description | |
| 2 | Q88IR3_PSEPK | 94 | ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2936 PE=4 SV=1 | |
63| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPK | 216 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain KT2440) GN=ribH PE=3 SV=1 |
64| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88MT1_PSEPK | 213 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1487 PE=4 SV=1 |
65| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FS1_PSEPK | 212 | Isocitrate dehydrogenase, NADP-dependent, monomeric-type OS=Pseudomonas putida (strain KT2440) GN=PP_4012 PE=4 SV=1 |
66| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KG8_PSEPK | 209 | Outer membrane lipoprotein OprI OS=Pseudomonas putida (strain KT2440) GN=oprI PE=4 SV=1 |
67| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PE1_PSEPK | 208 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1 |
68| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YIDC_PSEPK | 208 | Membrane protein insertase YidC OS=Pseudomonas putida (strain KT2440) GN=yidC PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 68.1 | YIDC_PSEPK |
208 | 61994 | 6 (4) | 6 (4) | 0.16 | |
| Membrane protein insertase YidC OS=Pseudomonas putida (strain KT2440) GN=yidC PE=3 SV=1 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 364 |
322.7032 | 643.3918 | 643.3904 | 2.19 | 1 | 21 | 0.071 | 5Score > 22 indicates identity |
U | K.LAALKE.R | |
| 1051 |
951.4968 | 950.4895 | 950.4743 | 16.0 | 1 | 4 | 2.2 | 5Score > 20 indicates identity |
U | K.VSMKDIDK.G + Oxidation (M) | |
| 1693 |
624.8474 | 1247.6802 | 1247.6761 | 3.30 | 1 | 82 | 3.2e-008 | 1Score > 19 indicates identity |
U | K.VETSALLYAGPK.I | |
| 2676 |
860.9312 | 1719.8478 | 1719.8428 | 2.94 | 0 | 103 | 2.3e-010 | 1Score > 21 indicates identity Score > 19 indicates homology |
U | R.VYLAQSGLTGTDGPDAR.A | |
| 2857 |
903.4798 | 1804.9450 | 1804.9207 | 13.5 | 1 | 40 | 0.00049 | 1Score > 19 indicates identity |
U | K.SYQLADGQEQLVVDLK.F | |
| 3739 |
774.4151 | 2320.2235 | 2320.2063 | 7.40 | 1 | 39 | 0.00047 | 1Score > 18 indicates identity |
U | R.KDSQGNYIIGYTGPVISVPAGGK.V |
69| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL28_PSEPK | 206 | 50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1 |
70| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS7_PSEPK | 202 | 30S ribosomal protein S7 OS=Pseudomonas putida (strain KT2440) GN=rpsG PE=3 SV=1 |
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