MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 313)

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+61

Accession Score Description
1 Q88DD5_PSEPK 231 HflK protein OS=Pseudomonas putida (strain KT2440) GN=hflK PE=4 SV=1

+62

Accession Score Description
Family member distances as a dendrogram 1 Q88H57_PSEPK 217 Cobalamin biosynthesis protein CobW OS=Pseudomonas putida (strain KT2440) GN=cobW PE=4 SV=1
3 Q88JM1_PSEPK 47 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2628 PE=3 SV=1
4 Q88FE7_PSEPK 30 description
2 Q88IR3_PSEPK 94 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2936 PE=4 SV=1

+63

Accession Score Description
1 RISB_PSEPK 216 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain KT2440) GN=ribH PE=3 SV=1

+64

Accession Score Description
1 Q88MT1_PSEPK 213 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1487 PE=4 SV=1

-65

Accession Score Description
1 Q88FS1_PSEPK 212 Isocitrate dehydrogenase, NADP-dependent, monomeric-type OS=Pseudomonas putida (strain KT2440) GN=PP_4012 PE=4 SV=1
Score Mass Matches Sequences emPAI
65.1 Q88FS1_PSEPK 212 80078 12 (8) 12 (8) 0.25
Isocitrate dehydrogenase, NADP-dependent, monomeric-type OS=Pseudomonas putida (strain KT2440) GN=PP_4012 PE=4 SV=1

-12 peptide matches (12 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
369   323.7005 645.3864 645.3850 2.28 0 19 0.037 +1Score > 18 indicates identity U R.FAPLAK.T
382   329.7105 657.4064 657.4061 0.51 1 31 0.0058 +1Score > 21 indicates identity U R.IELVGK.D
424   339.6830 677.3514 677.3497 2.62 0 5 3 +7Score > 22 indicates identity U E.AGDIFR.M
552   369.1961 736.3776 736.3755 2.85 0 15 0.17 +1Score > 20 indicates identity U K.FLDTNK.S
818   429.7447 857.4748 857.4719 3.43 1 3 5.4 +9Score > 23 indicates identity U R.KVGGIDNR.G
937   456.7671 911.5196 911.5189 0.86 0 21 0.029 +1Score > 18 indicates identity U K.GSAVNPVLR.E
1002   470.3005 938.5864 938.5800 6.82 0 32 0.00058 +1Score > 13 indicates identity U R.ILAPVDAIK.F
1537   587.3129 1172.6112 1172.6037 6.45 1 64 3.2e-006 +1Score > 22 indicates identity U K.AALIEADDSLR.I
1718   633.8787 1265.7428 1265.7343 6.74 0 52 1e-005 +1Score > 15 indicates identity U K.LPNISASVPQLK.A
2015   472.5766 1414.7080 1414.7052 1.97 0 3 1.1 +2Score > 21 indicates identity
Score > 16 indicates homology		 U E.ALAAQTDDAALQAR.F + Deamidated (NQ)
3033   957.4962 1912.9778 1912.9492 15.0 1 46 0.00013 +1Score > 20 indicates identity U R.DYLTDLFPIMELGTSAK.M
3608   749.0474 2244.1204 2244.0923 12.5 1 75 1.9e-007 +1Score > 20 indicates identity U K.HAAALAEVGFNANNGIGDLYAR.I + Deamidated (NQ)

+66

Accession Score Description
1 Q88KG8_PSEPK 209 Outer membrane lipoprotein OprI OS=Pseudomonas putida (strain KT2440) GN=oprI PE=4 SV=1

+67

Accession Score Description
1 Q88PE1_PSEPK 208 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1

+68

Accession Score Description
1 YIDC_PSEPK 208 Membrane protein insertase YidC OS=Pseudomonas putida (strain KT2440) GN=yidC PE=3 SV=1

+69

Accession Score Description
1 RL28_PSEPK 206 50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1

+70

Accession Score Description
1 RS7_PSEPK 202 30S ribosomal protein S7 OS=Pseudomonas putida (strain KT2440) GN=rpsG PE=3 SV=1
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