MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 313)

Page: Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 32 Next 

+61

Accession Score Description
1 Q88DD5_PSEPK 231 HflK protein OS=Pseudomonas putida (strain KT2440) GN=hflK PE=4 SV=1

-62

Accession Score Description
Family member distances as a dendrogram 1 Q88H57_PSEPK 217 Cobalamin biosynthesis protein CobW OS=Pseudomonas putida (strain KT2440) GN=cobW PE=4 SV=1
3 Q88JM1_PSEPK 47 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2628 PE=3 SV=1
4 Q88FE7_PSEPK 30 description
2 Q88IR3_PSEPK 94 ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2936 PE=4 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
Q88H57_PSEPK 217 38898 8 (5) 6 (4) 0.33
Cobalamin biosynthesis protein CobW OS=Pseudomonas putida (strain KT2440) GN=cobW PE=4 SV=1
Q88IR3_PSEPK 94 58936 2 (2) 2 (2) 0.08
ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2936 PE=4 SV=1
Q88JM1_PSEPK 47 71876 6 (2) 6 (2) 0.06
ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2628 PE=3 SV=1
Q88FE7_PSEPK 30 0 7 (2) 7 (2) 0.08
description

-21 peptide matches (20 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 4 Peptide
194   459.2560 458.2487 458.2489 -0.35 0 1 0.78 +1Score > 13 indicates identity U X R.QALGA.-
286   587.3518 586.3445 586.3438 1.16 1 8 0.76 +1Score > 25 indicates identity
Score > 20 indicates homology		 X R.ELVAR.R
289   589.3196 588.3123 588.3119 0.74 0 25 0.051 +3Score > 25 indicates identity U X E.VDDLK.V
306   302.1956 602.3766 602.3752 2.48 0 35 0.00046 +1Score > 14 indicates identity U X X K.TTLLR  
368   323.6927 645.3708 645.3810 -15.7 1 21 0.06 +1Score > 21 indicates identity U X E.KTLQR.I + Deamidated (NQ)
377   326.6575 651.3004 651.2976 4.31 0 1 3.7 +4Score > 20 indicates identity X E.FSGGQR.Q + Deamidated (NQ)
378   652.3913 651.3840 651.3929 -13.6 1 5 0.74 +10Score > 16 indicates identity U X R.RIAHR.V
728   827.5371 826.5298 826.5137 19.5 1 26 0.0092 +2Score > 19 indicates identity U X X R.QRIAIAR  
774   845.4495 844.4422 844.4290 15.6 0 15 0.4 +5Score > 23 indicates identity U X R.IDGQNVAK.V + Deamidated (NQ)
827   430.7229 859.4312 859.4300 1.41 0 5 1.1 +2Score > 23 indicates identity
Score > 17 indicates homology		 U X K.LFSHSNR.E
988   466.2597 930.5048 930.5134 -9.22 0 14 0.35 +2Score > 22 indicates identity U X R.LISSQGGIR.F + Deamidated (NQ)
1023   471.7919 941.5692 941.5658 3.63 0 45 0.00011 +1Score > 18 indicates identity U X R.LLVQGVGTR.F
1023   471.7919 941.5692 941.5698 -0.60 0 12 0.25 +3Score > 18 indicates identity U X R.LLPYPLAR.H
1238   521.8379 1041.6612 1041.6546 6.38 0 37 0.00021 +1Score > 13 indicates identity U X K.STLINLLLR.F
1382 +1 558.3036 1114.5926 1114.5870 5.09 1 41 0.00018 +1Score > 22 indicates identity
Score > 16 indicates homology		 U X K.ADLIDAEGLAK.V
2159   498.5883 1492.7431 1492.7192 16.0 0 4 0.44 +1Score > 21 indicates identity
Score > 13 indicates homology		 U X E.NIGTVQDGMATLTR.G + Deamidated (NQ); Oxidation (M)
2503   823.4295 1644.8444 1644.8319 7.65 1 7 1.5 +2Score > 21 indicates identity U X R.IDGQNVAKVTQDSLR.S + 2 Deamidated (NQ)
2683   862.9851 1723.9556 1723.9468 5.12 1 104 1.2e-010 +1Score > 18 indicates identity U X R.LVLIGQDLDAAQLEAR.L
2684   575.6609 1723.9609 1723.9468 8.15 1 57 4.6e-006 +1Score > 17 indicates identity U X R.LVLIGQDLDAAQLEAR.L
4525   1082.9309 3245.7709 3245.7183 16.2 0 73 6.5e-008 +1Score > 14 indicates identity U X R.VLLAQALFSDPDVLLLDEPTNHLDINTIR.W + Deamidated (NQ)

4 subsets and intersections (18 subset proteins in total)

Score Mass Subset of
CYSA_PSEPK 45 0 62.1, 62.2, 62.3
description
Q88C24_PSEPK 35 0 62.1
description
6 samesets of Q88C24_PSEPK
Q88KQ0_PSEPK 35 70282
ABC efflux transporter, permease/ATP-binding protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2240 PE=3 SV=1
Q88P94_PSEPK 35 0
description
Q88CP3_PSEPK 35 0
description
Q88DI0_PSEPK 35 0
description
Q88GS6_PSEPK 35 0
description
Q88LI4_PSEPK 35 0
description
MSBA_PSEPK 26 0 62.3
description
7 samesets of MSBA_PSEPK
Q88M15_PSEPK 26 0
description
Q88DA0_PSEPK 26 0
description
Q88PH2_PSEPK 26 36017
Dipeptide ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=dppF PE=3 SV=1
Q88L18_PSEPK 26 0
description
Q88EC4_PSEPK 26 67706
ABC transporter, ATP-binding protein/permease protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4542 PE=3 SV=1
Q88H94_PSEPK 26 0
description
Q88JT7_PSEPK 26 0
description
Q88DQ0_PSEPK 25 0 62.4
description
1 sameset of Q88DQ0_PSEPK
Q88LU8_PSEPK 25 0
description

+63

Accession Score Description
1 RISB_PSEPK 216 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain KT2440) GN=ribH PE=3 SV=1

+64

Accession Score Description
1 Q88MT1_PSEPK 213 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1487 PE=4 SV=1

+65

Accession Score Description
1 Q88FS1_PSEPK 212 Isocitrate dehydrogenase, NADP-dependent, monomeric-type OS=Pseudomonas putida (strain KT2440) GN=PP_4012 PE=4 SV=1

+66

Accession Score Description
1 Q88KG8_PSEPK 209 Outer membrane lipoprotein OprI OS=Pseudomonas putida (strain KT2440) GN=oprI PE=4 SV=1

+67

Accession Score Description
1 Q88PE1_PSEPK 208 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1

+68

Accession Score Description
1 YIDC_PSEPK 208 Membrane protein insertase YidC OS=Pseudomonas putida (strain KT2440) GN=yidC PE=3 SV=1

+69

Accession Score Description
1 RL28_PSEPK 206 50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1

+70

Accession Score Description
1 RS7_PSEPK 202 30S ribosomal protein S7 OS=Pseudomonas putida (strain KT2440) GN=rpsG PE=3 SV=1
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