MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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61| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DD5_PSEPK | 231 | HflK protein OS=Pseudomonas putida (strain KT2440) GN=hflK PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 61.1 | Q88DD5_PSEPK |
231 | 44734 | 8 (5) | 7 (5) | 0.28 | |
| HflK protein OS=Pseudomonas putida (strain KT2440) GN=hflK PE=4 SV=1 | |||||||
8 peptide matches (8 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 599 |
382.7204 | 763.4262 | 763.4228 | 4.51 | 1 | 26 | 0.014 | 1Score > 20 indicates identity |
U | K.LLAEYR.K | |
| 727 |
414.2070 | 826.3994 | 826.3973 | 2.55 | 0 | 12 | 0.29 | 1Score > 19 indicates identity |
U | R.FLDNYR.T | |
| 732 |
828.4015 | 827.3942 | 827.3813 | 15.6 | 0 | 7 | 0.51 | 3Score > 17 indicates identity |
U | R.FLDNYR.T + Deamidated (NQ) | |
| 1385 |
558.7720 | 1115.5294 | 1115.5247 | 4.23 | 1 | 10 | 0.67 | 1Score > 21 indicates identity |
U | K.GPPDLDEAFR.K | |
| 2118 |
738.3949 | 1474.7752 | 1474.7667 | 5.77 | 0 | 35 | 0.0016 | 1Score > 20 indicates identity Score > 19 indicates homology |
U | K.DGQSNLLYLPLDK.M | |
| 2658 |
856.9705 | 1711.9264 | 1711.9217 | 2.77 | 0 | 117 | 8.7e-012 | 1Score > 19 indicates identity |
U | R.TGITVTQVNVQSAAAPR.E | |
| 2901 |
609.9793 | 1826.9161 | 1826.9122 | 2.10 | 0 | 82 | 3.8e-008 | 1Score > 20 indicates identity |
U | R.KPSVPTTSASPSANDAAAR.A | |
| 4342 |
1004.1912 | 3009.5518 | 3009.5043 | 15.8 | 1 | 48 | 3.9e-005 | 1Score > 17 indicates identity |
U | K.ISNLQDFVLNVDQPEVSLQHATDSALR.H + Deamidated (NQ) |
62| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88H57_PSEPK | 217 | Cobalamin biosynthesis protein CobW OS=Pseudomonas putida (strain KT2440) GN=cobW PE=4 SV=1 | |
| 3 | Q88JM1_PSEPK | 47 | ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2628 PE=3 SV=1 | |
| 4 | Q88FE7_PSEPK | 30 | description | |
| 2 | Q88IR3_PSEPK | 94 | ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_2936 PE=4 SV=1 | |
63| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPK | 216 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain KT2440) GN=ribH PE=3 SV=1 |
64| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88MT1_PSEPK | 213 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1487 PE=4 SV=1 |
65| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FS1_PSEPK | 212 | Isocitrate dehydrogenase, NADP-dependent, monomeric-type OS=Pseudomonas putida (strain KT2440) GN=PP_4012 PE=4 SV=1 |
66| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88KG8_PSEPK | 209 | Outer membrane lipoprotein OprI OS=Pseudomonas putida (strain KT2440) GN=oprI PE=4 SV=1 |
67| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PE1_PSEPK | 208 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1 |
68| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YIDC_PSEPK | 208 | Membrane protein insertase YidC OS=Pseudomonas putida (strain KT2440) GN=yidC PE=3 SV=1 |
69| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL28_PSEPK | 206 | 50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1 |
70| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS7_PSEPK | 202 | 30S ribosomal protein S7 OS=Pseudomonas putida (strain KT2440) GN=rpsG PE=3 SV=1 |
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