MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
Not what you expected? Try 
the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88LM4_PSEPK | 257 | Ribonuclease E OS=Pseudomonas putida (strain KT2440) GN=rne PE=3 SV=1 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NS3_PSEPK | 251 | Outer membrane lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1131 PE=4 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88HA5_PSEPK | 251 | Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_3455 PE=4 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PN7_PSEPK | 248 | Cytochrome o ubiquinol oxidase, subunit II OS=Pseudomonas putida (strain KT2440) GN=cyoA PE=4 SV=1 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL13_PSEPK | 243 | 50S ribosomal protein L13 OS=Pseudomonas putida (strain KT2440) GN=rplM PE=3 SV=1 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PU5_PSEPK | 243 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0753 PE=4 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS11_PSEPK | 238 | 30S ribosomal protein S11 OS=Pseudomonas putida (strain KT2440) GN=rpsK PE=3 SV=1 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEPK | 237 | 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSEPK | 234 | 30S ribosomal protein S20 OS=Pseudomonas putida (strain KT2440) GN=rpsT PE=3 SV=1 |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DM2_PSEPK | 233 | D-alanyl-D-alanine carboxypeptidase OS=Pseudomonas putida (strain KT2440) GN=dacA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 60.1 | Q88DM2_PSEPK |
233 | 42375 | 11 (8) | 10 (8) | 0.53 | |
| D-alanyl-D-alanine carboxypeptidase OS=Pseudomonas putida (strain KT2440) GN=dacA PE=3 SV=1 | |||||||
11 peptide matches (11 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 493 |
356.6752 | 711.3358 | 711.3340 | 2.56 | 1 | 6 | 0.76 | 2Score > 18 indicates identity |
U | E.EGGFFR.R | |
| 705 |
407.7529 | 813.4912 | 813.4861 | 6.35 | 0 | 34 | 0.0028 | 1Score > 21 indicates identity |
U | R.NLLLWR.D | |
| 726 |
413.7564 | 825.4982 | 825.4960 | 2.74 | 0 | 37 | 0.00047 | 1Score > 16 indicates identity |
U | R.LPPASLTK.L | |
| 839 |
435.2490 | 868.4834 | 868.4807 | 3.18 | 0 | 39 | 0.00038 | 1Score > 17 indicates identity |
U | K.LLTYGFR.F | |
| 981 |
929.5444 | 928.5371 | 928.5454 | -8.89 | 1 | 4 | 1.7 | 4Score > 21 indicates identity Score > 18 indicates homology |
U | M.NITNLAKR.L | |
| 989 |
311.1790 | 930.5152 | 930.5134 | 1.91 | 1 | 4 | 3.2 | 5Score > 21 indicates identity |
U | M.NITNLAKR.L + 2 Deamidated (NQ) | |
| 1346 |
549.2781 | 1096.5416 | 1096.5342 | 6.83 | 1 | 27 | 0.0091 | 1Score > 19 indicates identity |
U | K.EFFWNNIK.Q | |
| 1649 |
615.3323 | 1228.6500 | 1228.6452 | 3.96 | 1 | 51 | 6.1e-005 | 1Score > 21 indicates identity |
U | K.GTELTQAPVWK.G | |
| 1850 |
669.8250 | 1337.6354 | 1337.6292 | 4.66 | 1 | 58 | 1e-005 | 1Score > 21 indicates identity |
U | R.FFETQTFYQK.G | |
| 2241 |
760.9114 | 1519.8082 | 1519.7995 | 5.79 | 1 | 76 | 8.9e-008 | 1Score > 20 indicates identity Score > 18 indicates homology |
U | R.LIAVVFGTNSEQSR.A | |
| 2852 |
601.9913 | 1802.9521 | 1802.9203 | 17.6 | 0 | 43 | 0.00021 | 1Score > 19 indicates identity |
U | R.AIINVDPAHYAIYSQK.E + Deamidated (NQ) |
Not what you expected? Try the select summary.
Mascot: http://www.matrixscience.com/