MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 313)

Page: 1 2 3 4 5 6 … 32 Next 

+1

Accession Score Description
1 Q88NM2_PSEPK 4307 Outer membrane protein H1 OS=Pseudomonas putida (strain KT2440) GN=oprH PE=4 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 2609 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
2 EFTU1_PSEPK 2412 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=1 SV=1

+3

Accession Score Description
1 ATPB_PSEPK 2344 ATP synthase subunit beta OS=Pseudomonas putida (strain KT2440) GN=atpD PE=3 SV=1

+4

Accession Score Description
1 Q88ES5_PSEPK 2322 Flagellin OS=Pseudomonas putida (strain KT2440) GN=fliC PE=3 SV=1

+5

Accession Score Description
1 Q88L46_PSEPK 1745 Outer membrane protein OprF OS=Pseudomonas putida (strain KT2440) GN=oprF PE=3 SV=1

-6

Accession Score Description
1 ATPA_PSEPK 1711 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
Score Mass Matches Sequences emPAI
6.1 ATPA_PSEPK 1711 55489 64 (55) 29 (24) 3.11
ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1

-64 peptide matches (42 non-duplicate, 22 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
286   587.3518 586.3445 586.3438 1.16 1 8 0.76 +1Score > 25 indicates identity
Score > 20 indicates homology		 K.ELLGR.V
301 +1 301.6854 601.3562 601.3548 2.49 0 22 0.081 +2Score > 23 indicates identity K.LSGGIR.T
302   602.3646 601.3573 601.3548 4.28 0 22 0.076 +1Score > 23 indicates identity K.LSGGIR.T
310   606.2899 605.2826 605.2809 2.80 0 10 0.45 +1Score > 19 indicates identity U K.ATQTW.-
489   354.1963 706.3780 706.3762 2.61 0 32 0.0033 +1Score > 20 indicates identity U K.QAVAYR.Q
490   707.3864 706.3791 706.3762 4.13 0 25 0.016 +1Score > 19 indicates identity U K.QAVAYR.Q
509   360.7031 719.3916 719.3887 4.03 1 26 0.016 +1Score > 20 indicates identity U R.VTELMK.Q
534   365.7331 729.4516 729.4497 2.66 1 15 0.15 +1Score > 19 indicates identity U K.KLSGGIR.T
707   408.2351 814.4556 814.4548 0.98 1 22 0.078 +1Score > 23 indicates identity U R.ELIIGDR.Q
708   815.4647 814.4574 814.4548 3.16 1 45 0.00039 +1Score > 23 indicates identity U R.ELIIGDR.Q
717   822.4489 821.4416 821.4395 2.55 0 36 0.0011 +1Score > 19 indicates identity U R.TALAQYR.E
718   411.7283 821.4420 821.4395 3.07 0 39 0.00056 +1Score > 19 indicates identity U R.TALAQYR.E
767   842.5493 841.5420 841.5385 4.17 0 43 8.5e-005 +1Score > 15 indicates identity U R.QISLLLR.R
770 +2 421.7799 841.5452 841.5385 8.00 0 41 7.6e-005 +1Score > 13 indicates identity U R.QISLLLR.R
806 +2 427.7725 853.5304 853.5273 3.69 1 43 6.1e-005 +1Score > 13 indicates identity U R.ILEVPVGK.E
808   854.5380 853.5307 853.5273 4.01 1 45 3.4e-005 +1Score > 13 indicates identity U R.ILEVPVGK.E
824   859.5016 858.4943 858.4923 2.35 0 39 0.001 +1Score > 21 indicates identity U R.STVANIVR.K
825 +1 430.2549 858.4952 858.4923 3.42 0 39 0.00085 +1Score > 21 indicates identity U R.STVANIVR.K
898   447.7406 893.4666 893.4607 6.67 0 36 0.0013 +1Score > 20 indicates identity U K.FTNGAVTGK.T
899   448.2292 894.4438 894.4447 -0.95 0 39 0.00048 +1Score > 20 indicates identity
Score > 18 indicates homology		 U K.FTNGAVTGK.T + Deamidated (NQ)
900   897.5327 896.5254 896.5232 2.45 0 21 0.027 +1Score > 18 indicates identity U K.VAPGVIWR.K
902 +2 449.2711 896.5276 896.5232 4.93 0 46 5.9e-005 +1Score > 17 indicates identity U K.VAPGVIWR.K
940   457.7245 913.4344 913.4327 1.89 1 24 0.018 +1Score > 19 indicates identity U R.DHAELMAK.I
1241   522.7797 1043.5448 1043.5434 1.42 0 37 0.0021 +1Score > 23 indicates identity U K.SVDAMIPVGR.G
1365 +3 553.8141 1105.6136 1105.6019 10.6 1 43 0.00021 +1Score > 19 indicates identity U R.GFLIDVEVSK.I
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_1_20250714114344.raw

Score > 22 indicates identity

Score > 18 indicates homology
-0.57 0 9 0.4 1 VGDIITITLNE   + Deamidated (NQ)
4.39 0 5 1.1 2 LAAFEPLWIE  
-4.51 1 3 1.7 3 QNKLDIMIGR   + Deamidated (NQ)
-11.1 0 2 2.1 4 IAANNYLRPR   + Deamidated (NQ)
1568   594.8212 1187.6278 1187.6510 -19.5 0 1 2.4 -5Score > 22 indicates identity
Score > 18 indicates homology		 U E.GTVVSVSDGIVR.I
-4.51 1 0 3.2 6 QNKLDIMIGR   + Deamidated (NQ)
1581 +2 599.3290 1196.6434 1196.6401 2.79 0 64 1.9e-006 +1Score > 19 indicates identity U R.VVDALGNPIDGK.G
1623 +1 609.3114 1216.6082 1216.6048 2.86 0 70 8.4e-007 +1Score > 22 indicates identity U R.IDNLDVSSQAR.N
1764 +1 644.8574 1287.7002 1287.6856 11.3 0 53 2.6e-005 +1Score > 19 indicates identity U K.TAMAIDAIINQK.D
1771 +1 647.3045 1292.5944 1292.5885 4.64 1 95 1.8e-009 +1Score > 20 indicates identity U K.GDFNDEIDAGLK.A
1837   665.3377 1328.6608 1328.6572 2.74 1 94 3e-009 +1Score > 21 indicates identity U K.GPLGNTQTDAVEK.V
1865   675.3582 1348.7018 1348.6987 2.35 1 95 2.5e-009 +1Score > 21 indicates identity U R.KSVDQPVQTGYK.S
1866   450.5753 1348.7041 1348.6987 3.99 1 33 0.0032 +1Score > 21 indicates identity U R.KSVDQPVQTGYK.S
2048   477.5852 1429.7338 1429.7273 4.50 1 24 0.029 +1Score > 22 indicates identity
Score > 21 indicates homology		 U K.GRIDNLDVSSQAR.N
2049   715.8743 1429.7340 1429.7273 4.69 1 74 3.7e-007 +1Score > 22 indicates identity U K.GRIDNLDVSSQAR.N
2050 +1 716.3784 1430.7422 1430.7365 3.99 1 78 1e-007 +1Score > 21 indicates identity U R.NEGTVVSVSDGIVR.I
2058   719.3671 1436.7196 1436.7035 11.3 1 48 0.0001 +1Score > 21 indicates identity U R.GEDALIVYDDLSK.Q
2291 +2 518.5873 1552.7401 1552.7310 5.82 1 52 3.6e-005 +1Score > 20 indicates identity U R.EAYPGDVFYLHSR.L
2292   777.3774 1552.7402 1552.7310 5.93 1 86 1.3e-008 +1Score > 20 indicates identity U R.EAYPGDVFYLHSR.L
2425 +1 806.9344 1611.8542 1611.8409 8.27 1 98 9.5e-010 +1Score > 20 indicates identity U K.IGSFEQALIAFFNR.D
2426 +2 538.2935 1611.8587 1611.8409 11.0 1 78 5.2e-008 +1Score > 20 indicates identity
Score > 18 indicates homology		 U K.IGSFEQALIAFFNR.D
3339   701.3486 2101.0240 2100.9859 18.1 1 35 0.00061 +1Score > 20 indicates identity
Score > 15 indicates homology		 U K.QYAPMSIADMALSLYAAER.G + Deamidated (NQ)

+7

Accession Score Description
1 Q88FB0_PSEPK 1295 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas putida (strain KT2440) GN=kgdB PE=3 SV=1

+8

Accession Score Description
1 Q88FA7_PSEPK 1233 Succinate dehydrogenase flavoprotein subunit OS=Pseudomonas putida (strain KT2440) GN=sdhA PE=3 SV=1

+9

Accession Score Description
1 RPOB_PSEPK 843 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

+10

Accession Score Description
1 Q88FA8_PSEPK 775 Succinate dehydrogenase, iron-sulfur protein OS=Pseudomonas putida (strain KT2440) GN=sdhB PE=4 SV=1
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