MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88LM4_PSEPK | 257 | Ribonuclease E OS=Pseudomonas putida (strain KT2440) GN=rne PE=3 SV=1 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NS3_PSEPK | 251 | Outer membrane lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1131 PE=4 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88HA5_PSEPK | 251 | Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_3455 PE=4 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PN7_PSEPK | 248 | Cytochrome o ubiquinol oxidase, subunit II OS=Pseudomonas putida (strain KT2440) GN=cyoA PE=4 SV=1 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL13_PSEPK | 243 | 50S ribosomal protein L13 OS=Pseudomonas putida (strain KT2440) GN=rplM PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 55.1 | RL13_PSEPK |
243 | 15852 | 14 (10) | 9 (6) | 1.64 | |
| 50S ribosomal protein L13 OS=Pseudomonas putida (strain KT2440) GN=rplM PE=3 SV=1 | |||||||
14 peptide matches (11 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 257 |
556.3223 | 555.3150 | 555.3129 | 3.86 | 0 | 24 | 0.0067 | 1Score > 15 indicates identity |
U | K.NPLGR.D | |
| 375 |
326.1716 | 650.3286 | 650.3275 | 1.74 | 1 | 9 | 0.83 | 2Score > 21 indicates identity |
U | E.INFEK.L + Deamidated (NQ) | |
| 376 |
651.3369 | 650.3296 | 650.3275 | 3.24 | 1 | 16 | 0.18 | 2Score > 21 indicates identity |
U | E.INFEK.L + Deamidated (NQ) | |
| 533 |
365.2357 | 728.4568 | 728.4657 | -12.1 | 1 | 8 | 0.93 | 4Score > 21 indicates identity |
U | E.IATRLR.G | |
| 586 |
759.4636 | 758.4563 | 758.4538 | 3.33 | 1 | 44 | 0.00015 | 1Score > 18 indicates identity |
U | R.VIETAVK.G | |
| 664 |
397.2092 | 792.4038 | 792.4018 | 2.63 | 0 | 3 | 3.3 | 8Score > 21 indicates identity |
U | K.TFTAKPE.T | |
| 850 |
874.5008 | 873.4935 | 873.4920 | 1.79 | 1 | 53 | 3e-005 | 1Score > 20 indicates identity |
U | R.LATEIATR.L | |
| 852 |
1 |
437.7545 | 873.4944 | 873.4920 | 2.85 | 1 | 42 | 0.00038 | 1Score > 20 indicates identity |
U | R.LATEIATR.L |
| 1400 |
374.5459 | 1120.6159 | 1120.6128 | 2.72 | 1 | 22 | 0.022 | 1Score > 18 indicates identity |
U | K.TFTAKPETVK.R | |
| 2284 |
2 |
774.8990 | 1547.7834 | 1547.7733 | 6.59 | 1 | 64 | 1.3e-006 | 1Score > 21 indicates identity Score > 18 indicates homology |
U | R.EWFVVDAAGQTLGR.L |
| 3072 |
487.5048 | 1945.9901 | 1945.9646 | 13.1 | 1 | 28 | 0.0087 | 1Score > 19 indicates identity |
U | K.VYAGAAHPHTAQQPQELK.I + Deamidated (NQ) |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PU5_PSEPK | 243 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0753 PE=4 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS11_PSEPK | 238 | 30S ribosomal protein S11 OS=Pseudomonas putida (strain KT2440) GN=rpsK PE=3 SV=1 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEPK | 237 | 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSEPK | 234 | 30S ribosomal protein S20 OS=Pseudomonas putida (strain KT2440) GN=rpsT PE=3 SV=1 |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DM2_PSEPK | 233 | D-alanyl-D-alanine carboxypeptidase OS=Pseudomonas putida (strain KT2440) GN=dacA PE=3 SV=1 |
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