MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita1
MS data file : PRT1270_T-BRSC_1_20250714114344.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 12 Aug 2025 at 22:47:09 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,938

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 313)

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+51

Accession Score Description
1 Q88LM4_PSEPK 257 Ribonuclease E OS=Pseudomonas putida (strain KT2440) GN=rne PE=3 SV=1

+52

Accession Score Description
1 Q88NS3_PSEPK 251 Outer membrane lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1131 PE=4 SV=1

-53

Accession Score Description
1 Q88HA5_PSEPK 251 Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_3455 PE=4 SV=1
Score Mass Matches Sequences emPAI
53.1 Q88HA5_PSEPK 251 42149 9 (5) 8 (5) 0.30
Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_3455 PE=4 SV=1

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
404   671.3745 670.3672 670.3650 3.35 0 7 0.65 +8Score > 18 indicates identity U E.QAVQPK.G + Deamidated (NQ)
1297   358.5161 1072.5265 1072.5149 10.8 0 0 1.1 +2Score > 22 indicates identity
Score > 13 indicates homology		 U E.LQQVGDGQAR.A + 2 Deamidated (NQ)
1370   369.8900 1106.6482 1106.6448 3.04 1 36 0.00039 +1Score > 15 indicates identity U R.VVVEGLQHVK.A
1424   566.2872 1130.5598 1130.5568 2.73 0 6 1.1 +2Score > 22 indicates identity
Score > 19 indicates homology		 U R.QVVLGSAQGDR.W + 2 Deamidated (NQ)
1425   1131.5677 1130.5604 1130.5568 3.24 0 5 2.6 +5Score > 22 indicates identity U R.QVVLGSAQGDR.W + 2 Deamidated (NQ)
2256   766.9337 1531.8528 1531.8358 11.1 0 79 3.4e-008 +1Score > 17 indicates identity U R.LNLGYATVTAPISGR.I
2503   823.4295 1644.8444 1644.8359 5.20 0 91 5.9e-009 +1Score > 21 indicates identity U R.ATLIQDDGSAYPLPGK.L
3046   960.4902 1918.9658 1918.9312 18.0 1 48 8.8e-005 +1Score > 20 indicates identity
Score > 20 indicates homology		 U R.SEFPNPDLDLLPGSYVR.V + Deamidated (NQ)
3186   673.7033 2018.0881 2018.0684 9.74 0 70 3.5e-007 +1Score > 18 indicates identity U K.LLFSDISVDPSTNQITLR.S

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q88J32_PSEPK 79 39677 53.1
Multidrug efflux RND membrane fusion protein MexC OS=Pseudomonas putida (strain KT2440) GN=mexC PE=4 SV=1

+54

Accession Score Description
1 Q88PN7_PSEPK 248 Cytochrome o ubiquinol oxidase, subunit II OS=Pseudomonas putida (strain KT2440) GN=cyoA PE=4 SV=1

+55

Accession Score Description
1 RL13_PSEPK 243 50S ribosomal protein L13 OS=Pseudomonas putida (strain KT2440) GN=rplM PE=3 SV=1

+56

Accession Score Description
1 Q88PU5_PSEPK 243 Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0753 PE=4 SV=1

+57

Accession Score Description
1 RS11_PSEPK 238 30S ribosomal protein S11 OS=Pseudomonas putida (strain KT2440) GN=rpsK PE=3 SV=1

+58

Accession Score Description
1 RL1_PSEPK 237 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1

+59

Accession Score Description
1 RS20_PSEPK 234 30S ribosomal protein S20 OS=Pseudomonas putida (strain KT2440) GN=rpsT PE=3 SV=1

+60

Accession Score Description
1 Q88DM2_PSEPK 233 D-alanyl-D-alanine carboxypeptidase OS=Pseudomonas putida (strain KT2440) GN=dacA PE=3 SV=1
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