MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita1 |
| MS data file | : | PRT1270_T-BRSC_1_20250714114344.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 12 Aug 2025 at 22:47:09 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,938 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88LM4_PSEPK | 257 | Ribonuclease E OS=Pseudomonas putida (strain KT2440) GN=rne PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 51.1 | Q88LM4_PSEPK |
257 | 121004 | 10 (7) | 9 (6) | 0.14 | |
| Ribonuclease E OS=Pseudomonas putida (strain KT2440) GN=rne PE=3 SV=1 | |||||||
10 peptide matches (10 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 412 |
336.7136 | 671.4126 | 671.4078 | 7.17 | 1 | 7 | 1.5 | 9Score > 21 indicates identity |
E.VQRLR.D + Deamidated (NQ) | ||
| 1279 |
534.2614 | 1066.5082 | 1066.5117 | -3.25 | 0 | 1 | 4.4 | 3Score > 20 indicates identity |
U | R.QVMPQYASK.V + Oxidation (M) | |
| 1620 |
608.3609 | 1214.7072 | 1214.6870 | 16.6 | 1 | 60 | 1.8e-006 | 1Score > 15 indicates identity |
U | R.DVESLSLAILR.L | |
| 1745 |
639.3710 | 1276.7274 | 1276.7139 | 10.6 | 0 | 74 | 6.7e-008 | 1Score > 15 indicates identity |
U | K.GAALTTFISLAGR.Y | |
| 1871 |
676.8508 | 1351.6870 | 1351.6772 | 7.29 | 1 | 42 | 0.0004 | 1Score > 20 indicates identity |
U | K.LYEDSVPLFNR.F | |
| 2411 |
537.3190 | 1608.9352 | 1608.9239 | 7.00 | 1 | 32 | 0.00079 | 1Score > 13 indicates identity |
U | R.AQVPIPVAAFLLNEK.R | |
| 2416 |
805.9745 | 1609.9344 | 1609.9079 | 16.5 | 1 | 54 | 5.2e-006 | 1Score > 14 indicates identity |
U | R.AQVPIPVAAFLLNEK.R + Deamidated (NQ) | |
| 2716 |
867.4931 | 1732.9716 | 1732.9512 | 11.8 | 1 | 30 | 0.0019 | 1Score > 15 indicates identity |
U | R.AAPFLIYQESNVIIR.A | |
| 2882 |
909.4986 | 1816.9826 | 1816.9645 | 10.0 | 0 | 7 | 0.7 | 1Score > 18 indicates identity |
U | R.DIGGLIVIDFIDMTPAK.N | |
| 4122 |
913.5025 | 2737.4857 | 2737.4386 | 17.2 | 1 | 58 | 3.8e-006 | 1Score > 16 indicates identity |
U | R.VVDLPSGGSIVIDPTEALVSIDINSAR.A + Deamidated (NQ) |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NS3_PSEPK | 251 | Outer membrane lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1131 PE=4 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88HA5_PSEPK | 251 | Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_3455 PE=4 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PN7_PSEPK | 248 | Cytochrome o ubiquinol oxidase, subunit II OS=Pseudomonas putida (strain KT2440) GN=cyoA PE=4 SV=1 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL13_PSEPK | 243 | 50S ribosomal protein L13 OS=Pseudomonas putida (strain KT2440) GN=rplM PE=3 SV=1 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PU5_PSEPK | 243 | Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0753 PE=4 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS11_PSEPK | 238 | 30S ribosomal protein S11 OS=Pseudomonas putida (strain KT2440) GN=rpsK PE=3 SV=1 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEPK | 237 | 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSEPK | 234 | 30S ribosomal protein S20 OS=Pseudomonas putida (strain KT2440) GN=rpsT PE=3 SV=1 |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DM2_PSEPK | 233 | D-alanyl-D-alanine carboxypeptidase OS=Pseudomonas putida (strain KT2440) GN=dacA PE=3 SV=1 |
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